Kumari, SnehalChaudhary, NehaPriya, KhushbuRai, GeetaGautam, Dev Nath SinghSingh, Narendra Kumar2025-01-282025-01-2820239725938https://dl.bhu.ac.in/ir/handle/123456789/23663Tephrosia purpurea belongs to the family Fabaceae, is used for the treatment of inflammation, diabetes, cancer, chronic fever, boils, gingivitis etc. In the present study, three compounds (TP-1, TP-3 and TP-5) were isolated from ethyl acetate extract of the seeds of T. purpurea (EETP). Quantity of TP-3 (6.05%) in EETP was determined by HPLC. In-vitro anti-cancer activity of EETP and isolated compound TP-3 on SiHa cells as well as PBMCs (peripheral blood mononuclear cells) were evaluated by MTT (3-(4,5-di methyl thiazol-2-yl)-2,5-di phenyl tetrazolium bromide) assay. On the basis of spectroscopic analysis and physical properties, structure of isolated compounds TP-1, TP-3 and TP-5 were characterized as teclenone, pongamol and ?-sitosterol respectively. Isolated compound TP-3 showed cytotoxicity against SiHa cells with the IC50 33.06 �gmL-1. TP-3 showed significant cytotoxicity at the concentration range from 20-50 �gmL-1 as compared to control. Maximum cytotoxicity (72.75%) was observed at the concentration of 50 �gmL-1. TP-3 showed non-significant cytotoxicity against PBMCs cells at all the concentrations except at 50 �gmL-1 (cytotoxicity 24.19%). EETP showed significant cytotoxicity (IC50 value 113.63 �gmL-1) against SiHa cells at the concentration range from 75-150 �gmL-1 as compared to control. Maximum cytotoxicity (75.34%) was observed at the concentration of 150 �gmL-1. � 2023, National Institute of Science Communication and Policy Research. All rights reserved.enMTT assayPBMCs cellsSiHa CellsTeclenone?-sitosterolBook chapterhttps://doi.org/10.56042/ijtk.v22i1.33137