Rai N.Gupta P.Verma A.Singh S.Tiwari H.Kumar R.Singh S.K.Gautam V.2025-01-132025-01-132024978-981996263-1; 978-981996262-4https://dl.bhu.ac.in/ir/handle/123456789/1976Endophytic microorganisms establish symbiotic relationship with their host plants and also have a unique ability to mimic bioactive metabolite produced from host plants. Bioactive compounds produced from fungal endophyte have tendency to reduce metallic ions such as silver, gold, platinum, and zinc into nanoparticles. Biosynthesis of fungal endophyte-mediated silver nanoparticles (AgNPs) has evolved as a frontier of nanotechnology. For decades, silver has been observed as a nontoxic and harmless inorganic agent which is utilized as antibacterial or antifungal agent. Silver exhibits an exceptional physical, chemical, and a wide range of potential biological activities, predominantly in the form of nanoparticles. Although the effective biosynthesis of fungal endophytemediated AgNPs and their antiapoptotic and antiproliferative property have been accounted as of now, the exact mechanism of synthesis of nanoparticles remains unclear. The current chapter gives a brief outline of the possible mechanism of fungal endophyte-mediated synthesis of AgNPs and its anticancer properties. Moreover, it also provides an insight for the mycogenic synthesis and characterization of AgNPs mediated by fungal endophyte using various highthroughput techniques including UV-Visible spectrophotometer, Fourier transform infrared spectroscopy (FT-IR), high-resolution transmission electron microscopy (HR-TEM), scanning electron microscopy (SEM) equipped with energy dispersive X-ray analysis (EDAX), and X-ray Powder Diffraction (XRD). Additionally, it also describes bottlenecks in the field of myconanotechnology including manufacturing at large scale, improved stability, and reduced time to acquire compatible size and shape of AgNPs and their potential uses in cancer research. � Springer Nature Singapore Pte Ltd. 2024. All rights reserved.enAnticancerFTIRFungal endophyteSEMSilver nanoparticlesTEMBook chapter10.1007/978-981-99-6263-1_70