Browsing by Author "Anurag Mishra"

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An excellent stable fluorescent probe: Selective and sensitive detection of trace amounts of Hg+2 ions in natural source of water
(Elsevier B.V., 2017) Ranjana Singh; Sunil Kumar Rai; Manish Kumar Tiwari; Anurag Mishra; Ashish K. Tewari; Phool Chand Mishra; Ranjan K. Singh
The fluorescent and stable aqueous solution of 3-Cyano-4,6-dimethyl-2-pyridone (CDPy) has been used as a probe for selective detection of Hg+2 ions in natural river water. The fluorescence intensity of the probe was reduced on adding Hg+2 ions. The DFT and TD-DFT confirmed that the reduction in fluorescence intensity occurred due to excited state charge transfer between the CDPy molecule and Hg+2 ions. This procedure can be used to detect Hg+2 ions up to a detection limit ∼0.41 μM. It is reproducible with excellent stability (∼10 weeks) and applicable in a wide linear range (3–231 μM). © 2017
Characterisation of Two-Microlocal Spaces and Wavefront Sets by Wavelet Transforms and Their Application to Solutions of Schrödinger Equations
(Springer, 2025) Ashish Pathak; Anurag Mishra
In this paper, we characterise Hölder continuity, two-microlocal spaces, and the Sobolev wavefront set, and propose a novel representation of the solutions of Schrödinger equations for a free particle using the wavelet transform introduced by Moritoh in Moritoh (Tohoku Math J 47:555–565, 1995). Additionally, we discuss its application in determining the microlocally regular solutions to the Schrödinger equations of a free particle, providing a refined understanding of their microlocal regularity properties. © The Author(s), under exclusive licence to Springer Nature India Private Limited 2025.
Comprehensive analysis of wheat starch synthase III revealed existence of two copies differentially expressed under heat stress
(Akademiai Kiado ZRt., 2024) Vishnu Kumar; Bipramani Nameirakpam; Tamilarasi Murugesh; Pragya Pragya; Anurag Mishra; Sourav Panigrahi; Yaswant Kumar Pankaj; Rajeev Kumar
Starch is the most critical source of energy and constitutes 65–80% of the wheat grain. Starch synthase (SS) is a key regulator of starch metabolism. Among its five isoforms, SSIII plays a pivotal role in phytoglycogen accumulation. In wheat, it is associated with starch accumulation but is sensitive to heat stress. In silico characterization indicated two homologous copies of the TaSSIII genes (Triticum aestivum starch synthase III genes), TaSSIIIa and TaSSIIIb. TaSSIIIa shares maximum similarity with HvSSIIIa, whereas TaSSIIIb exhibits maximum similarity with OsSSIIIb. It encodes proteins with an N-terminal transit peptide, an SSIII specific domain, a C-terminal catalytic domain, and a conserved glycosyltransferase domain with variations among the homoeologs. The catalytic domain has N-glycosylation motifs, with different numbers in the two homologs. ADP glucose-binding motifs are present in the SS-CD and GT-1 domains. ADP-binding pockets are present at the C-terminus of the catalytic domains. Maximum heterogen ligands are found in TaSSIIIa1B and minimum in TaSSIIIb2B. TaSSIIIb is expressed in leaf, stem, root, spike, and grain, and in much higher amounts than TaSSIIIa. Pot-grown wheat genotypes showed heat stress-induced upregulation of the genes TaSSIIIa1D and TaSSIIIb2D in the flag leaf. The tolerant genotype showed a significantly higher fold increase in the transcript levels under heat stress. Under the stress in the field, transcript level change for TaSSIIIa1D was higher in peduncle as compared to flag leaf, and that of TaSSIIIb2D was not significantly different between tissues. Homoeologous copies of the gene have tissue and genotype-specific expression, are influenced by heat stress, and thus may play a role in the homeostasis of starch metabolism under stress. © Akadémiai Kiadó Zrt. 2024.
Comprehensive analysis of wheat starch synthase III revealed existence of two copies differentially expressed under heat stress
(Akademiai Kiado ZRt., 2025) Vishnu Kumar; Bipramani Nameirakpam; Tamilarasi Murugesh; Pragya Pragya; Anurag Mishra; Sourav Panigrahi; Yaswant Kumar Pankaj; Rajeev Kumar
Starch is the most critical source of energy and constitutes 65–80% of the wheat grain. Starch synthase (SS) is a key regulator of starch metabolism. Among its five isoforms, SSIII plays a pivotal role in phytoglycogen accumulation. In wheat, it is associated with starch accumulation but is sensitive to heat stress. In silico characterization indicated two homologous copies of the TaSSIII genes (Triticum aestivum starch synthase III genes), TaSSIIIa and TaSSIIIb. TaSSIIIa shares maximum similarity with HvSSIIIa, whereas TaSSIIIb exhibits maximum similarity with OsSSIIIb. It encodes proteins with an N-terminal transit peptide, an SSIII specific domain, a C-terminal catalytic domain, and a conserved glycosyltransferase domain with variations among the homoeologs. The catalytic domain has N-glycosylation motifs, with different numbers in the two homologs. ADP glucose-binding motifs are present in the SS-CD and GT-1 domains. ADP-binding pockets are present at the C-terminus of the catalytic domains. Maximum heterogen ligands are found in TaSSIIIa1B and minimum in TaSSIIIb2B. TaSSIIIb is expressed in leaf, stem, root, spike, and grain, and in much higher amounts than TaSSIIIa. Pot-grown wheat genotypes showed heat stress-induced upregulation of the genes TaSSIIIa1D and TaSSIIIb2D in the flag leaf. The tolerant genotype showed a significantly higher fold increase in the transcript levels under heat stress. Under the stress in the field, transcript level change for TaSSIIIa1D was higher in peduncle as compared to flag leaf, and that of TaSSIIIb2D was not significantly different between tissues. Homoeologous copies of the gene have tissue and genotype-specific expression, are influenced by heat stress, and thus may play a role in the homeostasis of starch metabolism under stress. © Akadémiai Kiadó Zrt. 2024.
Cytotoxic and apoptotic inducing activity of Amoora rohituka leaf extracts in human breast cancer cells
(Elsevier B.V., 2020) Rajesh Kumar Singh; Amit Ranjan; Akhileshwar Kumar Srivastava; Monika Singh; Anil Kumar Shukla; Neelam Atri; Anurag Mishra; Anil Kumar Singh; Santosh Kumar Singh
Background: Amoora rohituka is described in Ayurveda, an Indian traditional system of medicine for management of disorders of blood, diseases of eye, helminthiasis disease, ulcer, liver disorders and splenomegaly. However, the leaves were not reported to have anticancer properties till date. Objective: This study was carried out to evaluate the cytotoxic potential of leaf extracts of Amoora rohituka. Materials and methods: The leaves powder was macerated in petroleum ether, ethyl acetate and methanol and evaluated their anticancer activities in vitro. The phytochemical constituents of the active (ethyl acetate) extract were screened by FTIR analysis and phytochemical screening methods. Results: The ethyl acetate extract (RLEA) showed the presence of alkaloids, flavonoids, steroids, tannins, saponins and terpenoids. The RLEA exhibited high cytotoxic effect against human breast cancer cells, MCF-7 (IC50 = 9.81 μg/mL) and induced apoptosis by altering nuclear morphology and DNA laddering. Wound healing assays explained the potency of extract to decrease the cell migration. Conclusion: The extract of Amoora rohituka leaves exhibited anticancer activity with less toxicity and it could be used for development of alternative drugs in the treatment of human breast cancer. © 2019 Transdisciplinary University, Bangalore and World Ayurveda Foundation
Evidence-Based Therapeutic Potential of Natural Seed Oil of Desert Date/Ingudi (Balanites aegyptiaca Linn. Delile) in Chronic Diabetic Wound
(Springer, 2023) Sanjeev Kumar; Anurag Mishra; Kamal Nayan Dwivedi; Neelu Singh; Abhishek Kumar; Satyanam Kumar Bhartiya; S. Krishnamoorthi; Amit Kumar; Bharat Goel; Shreyans K. Jain
This study is to clarify the things said in Ayurveda and Sanskrit literature with evidence in today's context that Balanites aegyptiaca oil (BAO) was used on humans and animals to heal wounds without any side effects. BAO was tested for its wound-healing potential on a diabetic patient. The topical application of the BAO caused wound closure that was ascertained by histopathological changes that occurred during the treatment at the site of application. The phytochemical analysis of BAO resulted in the isolation of potential marker compounds such as diosgenin, caffeic acid, stigmasterol, and fatty acids that may be contributing factors to its wound healing potential. However, underlying mechanisms need to be explored to understand the mechanism behind their wound-healing property. © 2023, The Author(s), under exclusive licence to The National Academy of Sciences, India.
Green single-step hydrothermal synthesis of fluorescent carbon dots from Lantana camara flower for the effective fluorescent detection of Cr(VI) and live cell imaging
(Springer Science and Business Media Deutschland GmbH, 2025) Vikky Kumar Mahto; Vikas Kumar Singh; Vipendra Kumar Singh; Avinash K. Singh; Savita S. Singh; Arjun Kumar Mehara; Naina Rajak; Anurag Mishra; Neha Garg; Akanksha Upadhyay; Abhishek Rai; Ankit Kumar Singh
Chromium is one of the heavy metal ions showing high toxicity and mutagenicity. Owing to this, selective sensing of Cr(VI) from sample metrics is a challenging and tedious process. In the present work, we have synthesized fluorescent carbon dots (FCDs) using the flower of Lantana camara through a one-step hydrothermal method. Various spectroscopic techniques such as transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), EDAX, and Raman analysis well supported the successful synthesis of FCDs. The obtained FCDs revealed a bright blue color under UV-light exposure (@ 365 nm), with excellent optical properties and a fluorescence quantum yield of 29%. Furthermore, FCDs showed excellent fluorescence stability, high ionic strength, good water solubility, low cytotoxicity, and well biocompatibility. Therefore, the proposed FCDs were employed for selective turn-off sensing of toxic Cr(VI) in an aqueous medium with a limit of detection (LOD) of 0.10 µM. Interestingly, the low cytotoxicity and excellent biocompatibility enable the FCDs as a good candidate for cell imaging agents as well as intracellular turn-off sensing of Cr(VI) in HEK-293 cells. Thus, the present work significantly converts biomass of weed plants into a fluorescent probe in a simple and cost-effective way for the detection of Cr(VI) in water samples as well as in living cells. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
Green single-step hydrothermal synthesis of fluorescent carbon dots from Lantana camara flower for the effective fluorescent detection of Cr(VI) and live cell imaging
(Springer Science and Business Media Deutschland GmbH, 2024) Vikky Kumar Mahto; Vikas Kumar Singh; Vipendra Kumar Singh; Avinash Singh; Savita Singh; Arjun Kumar Mehara; Naina Rajak; Anurag Mishra; Neha Garg; Akanksha Upadhyay; Abhishek Rai; Ankit Kumar Singh
Chromium is one of the heavy metal ions showing high toxicity and mutagenicity. Owing to this, selective sensing of Cr(VI) from sample metrics is a challenging and tedious process. In the present work, we have synthesized fluorescent carbon dots (FCDs) using the flower of Lantana camara through a one-step hydrothermal method. Various spectroscopic techniques such as transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), EDAX, and Raman analysis well supported the successful synthesis of FCDs. The obtained FCDs revealed a bright blue color under UV-light exposure (@ 365 nm), with excellent optical properties and a fluorescence quantum yield of 29%. Furthermore, FCDs showed excellent fluorescence stability, high ionic strength, good water solubility, low cytotoxicity, and well biocompatibility. Therefore, the proposed FCDs were employed for selective turn-off sensing of toxic Cr(VI) in an aqueous medium with a limit of detection (LOD) of 0.10 µM. Interestingly, the low cytotoxicity and excellent biocompatibility enable the FCDs as a good candidate for cell imaging agents as well as intracellular turn-off sensing of Cr(VI) in HEK-293 cells. Thus, the present work significantly converts biomass of weed plants into a fluorescent probe in a simple and cost-effective way for the detection of Cr(VI) in water samples as well as in living cells. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
Paradendryphiella arenariae (MW504999) as a Novel Fungal Source of Tenuazonic Acid in Tomato (Lycopersicon esculentum)
(Springer, 2025) Ankita Kumari; Karuna Singh; Neha Tiwari; Diksha Katiyar; Satyendra Pratap Singh; Anurag Mishra
Tenuazonic acid (TeA) is a mycotoxin usually produced by Alternaria species. Its toxicological potency is considered to be the highest among all Alternaria-mycotoxins. The present study for the first time reports Paradendryphiella arenariae isolated from tomato (Lycopersicon esculentum) as a source of TeA mycotoxin, thus adding a new genus to the array of TeA-producing fungi. The study involves optimizing culture conditions for maximum TeA production, and employing analytical techniques to characterize the compound. Thin-layer chromatography and high-pressure liquid chromatography (HPLC) were employed for the isolation and characterization of the mycotoxin produced by P. arenariae. Structural elucidation was achieved using Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. Quantitative determination of TeA was conducted using HPLC with a standard TeA reference. The presence of TeA was further confirmed through electronspray ionization-mass spectrometry and high-resolution liquid chromatography-mass spectrometry. In cytotoxicity assays, the isolated TeA exhibited significant toxicity to murine splenocytes, with an IC50 of 25 µg/mL. This study highlights the need for vigilance regarding TeA contamination in food products. The identification of P. arenariae as a new source of TeA underscores the importance of expanding monitoring efforts to include diverse fungal species. Ensuring food safety through stringent regulations and routine testing is essential to mitigate health risks associated with TeA exposure. © Association of Microbiologists of India 2025.
Potable water production through a low-cost single chamber solar still in north India
(Springer, 2024) Vinay Mishra; Dhananjay Singh; Ram Sharan Singh; Deepak Singh; Eldon R. Rene; Balendu Shekher Giri; Sarthik Sharma; Anurag Mishra; Harishchandra Shukla
The main aim of this study is to evaluate the performance of a single slope solar still and to assess the effect of nanofluid on its performance. A single basin single slope solar still was designed and fabricated at the Department of Chemical Engineering, IET Lucknow. Its performance was assessed in terms of the yield of potable water. The effect of various climatic parameters was also studied. Al2O3 nanofluid was used to enhance the yield of the solar still. In the presence of nanofluid, the total yield of the solar still improved by 16.6%. Its economic feasibility was analyzed and reported. The portability of the small size of solar stills, its better economics, easy fabrication, and good performance make them very useful for industrial as well as household purposes. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
Stacked Ensemble with Machine Learning Regressors on Optimal Features (SMOF) of hyperspectral sensor PRISMA for inland water turbidity prediction
(Springer, 2024) Rajarshi Bhattacharjee; Shishir Gaur; Shard Chander; Anurag Ohri; Prashant K. Srivastava; Anurag Mishra
Leveraging hyperspectral data across various domains yields substantial benefits, yet managing many spectral bands and identifying the essential ones poses a formidable challenge. This study identifies the most relevant bands within a hyperspectral data cube for turbidity prediction in inland water. Nine machine learning regressors Cat Boost, Decision Trees, Extra Trees, Gradient Boost, Light Gradient Boost (LightGBM), Recursive Feature Elimination (RFE), Random Forest, Support Vector Regressor (SVR), and Xtreme Gradient Boost (XGBoost) have been used to compute the feature importance of the hyperspectral bands for predicting turbidity. Random Forest has outperformed the other models with a mean absolute percentage error (MAPE) of 1.61%, and the R2 of the linear fit is 0.96. Band 77, with a central wavelength of 1067.61 nm, is the most dominating band regarding feature importance. We have also developed a novel framework for turbidity prediction: Stacked Ensemble with Machine Learning Regressors on Optimal Features (SMOF). It employs a stacking ensemble of the nine regressors mentioned above with Random Forest as both base and meta-model, leveraging feature selection outputs. With this framework, the MAPE (%) reached 1.21, while the R2 stood at 0.95. The present study also presents a simple statistical algorithm to detect noisy bands in the Hyperspectral Precursor of the Application Mission (PRISMA) data cube. The approach assesses quadrat-wise intra-band spatial coherence using Renyi’s entropy thresholding for noisy band segregation. Radiometric calibration error and absorption due to water vapour are the two primary sources of noise within the data cube. Moreover, this research implements the open-source Water Colour Simulator (WASI) to simulate inland water spectra with varied proportions of turbidity. Overall, the study presents an approach to identify noisy bands and integrates the potential wavelengths for turbidity prediction of inland waters. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
Synthesis and enhanced photoluminescence properties of red emitting divalent ion (Ca2+) doped Eu:Y2O3 nanophosphors for optoelectronic applications
(Editorial Office of Chinese Rare Earths, 2022) Arpita Dwivedi; Monika Srivastava; Ankur Dwivedi; Amit Srivastava; Anurag Mishra; S.K. Srivastava
This work presents the synthesis of Y2O3:Eu3+,xCa2+ (x = 0 mol%, 1 mol%, 3 mol%, 5 mol%, 7 mol%, 9 mol%, 11 mol%) nanophosphors with enhanced photoluminescence properties through a facile solution combustion method for optoelectronic, display, and lighting applications. The X-ray diffraction (XRD) patterns of the proposed nanophosphor reveal its structural properties and crystalline nature. The transmission electron microscope (TEM) results confirm the change in the shape of the particle and aggregation of particles after co-doping with Ca2+. Fourier transform infrared spectroscopy (FTIR) and Raman vibrations also confirm the presence of Y–O vibration and subsequently explain the crystalline nature, structural properties, and purity of the samples. All the synthesized nanophosphors samples emit intense red emission at 613 nm (5D0→7F2) under excitation with 235, 394 and 466 nm wavelengths of Eu3+ ions. The photoluminescence (PL) emission spectra excited with 235 nm illustrate the highest emission peak with two other emission peaks excited with 466 and 394 nm that is 1.4 times higher than 466 nm and 1.9 times enhanced by 394 nm wavelength, respectively. The emission intensity of Y2O3:Eu3+,xCa2+ (5 mol%) is increased 8-fold as compared to Eu:Y2O3. Doping with Ca2+ ions enhances the emission intensity of Eu:Y2O3 nanophosphors due to an increase in energy transfer in Ca2+→Eu3+ through asymmetry in the crystal field and by introduction of radiative defect centers through oxygen vacancies in the yttria matrix. It is also observed that the optical band gap and the lifetime of the 5D0 level of Eu3+ ions in Y2O3:Eu3+,xCa2+ nanophosphor sample gets changed with a doping concentration of Ca2+ ions. Nanophosphor also reveals high thermal stability and quantum yield as estimating activation energy of 0.25 eV and 81%, respectively. CIE, CCT, and color purity values (>98%) show an improved red-emitting nanophosphor in the warm region of light, which makes this material superior with a specific potential application for UV-based white LEDs with security ink, display devices, and various other optoelectronics devices. © 2021 Chinese Society of Rare Earths
Unveiling the Influence of Glutathione in Suppressing the Conversion of Aspirin to Salicylic Acid: A Fluorescence and DFT Study
(Springer, 2024) Monalisha Nayak; Chandan Bhai Patel; Anurag Mishra; Ranjana Singh; Ranjan K. Singh
Aspirin is a commonly used nonsteroidal anti-inflammatory drug, associated with many adverse effects. The adverse effects of aspirin such as tinnitus, Reye’s syndrome and gastrointestinal bleeding are caused due to conversion of aspirin into its active metabolite salicylic acid after oral intake. Glutathione is a naturally occurring antioxidant produced by the liver and nerve cells in the central nervous system. It helps to metabolize toxins, break down free radicles, and support immune function. This study aims to investigate and explore the possibility of inhibiting aspirin to salicylic acid conversion in presence of glutathione at a molecular level using spectroscopic techniques such as UV–Visible absorption, time-Resolved and time-dependent fluorescence and theoretical DFT/ TD-DFT calculations. The results of steady state fluorescence spectroscopy and time-dependent fluorescence indicated that the aspirin to salicylic acid conversion is considerably inhibited in presence of glutathione. Further, the results presented here might have significant clinical implications for individuals with variations in glutathione level. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.