Browsing by Author "B. Geetha"

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Effect of cisplatin and other biological response modifiers on the activity of lysozyme, plasminogen activator and 5' nucleotidase by murine macrophages in vitro
(1990) B. Geetha; A. Sodhi
In vitro effect of cisplatin and other biological response modifiers has been studied. It is observed that in vitro treatment of macrophage monolayers with cisplatin, rIFN Y, LPS or MDP either alone or in combination showed significantly increased activity of lysozyme, plasminogen activator and decreased activity of 5' nucleotidase. Priming of macrophage with rIFN Y had a significant effect in enhancing the activity of lysozyme and plasminogen activator when subsequently treated with cisplatin, MDP or LPS.
Effect of cisplatin, lipopolysaccharide, muramyl dipeptide and recombinant interferon-gamma on murine macrophages in vitro. I. Macrophage-mediated tumor cell lysis
(1989) B. Geetha; A. Sodhi
Murine macrophage monolayers treated with cisplatin, lipopolysaccharide (LPS), muramyl dipeptide (MDP) or recombinant interferon-γ (rIFNγ) were observed to have significantly increased tumoricidal activity. rIFNγ had synergistic effects with cisplatin, LPS or MDP in activating macrophages. However, MDP showed much more pronounced synergism with cisplatin and LPS than with rIFNγ. Supernatants collected from these activated macrophage monolayers also showed increased tumoricidal activity. Tumor cell lysis mediated by cisplatin-treated macrophages did not require priming with rIFNγ though it may be necessary as a first signal for the increased macrophage activation with LPS and MDP.
Effect of cisplatin, lipopolysaccharide, muramyl dipeptide and recombinant interferon-gamma on murine macrophages in vitro. II. Production of H2O2, O2̄ and interleukin-1
(1989) A. Sodhi; B. Geetha
Murine macrophage monolayers treated with cisplatin, lipopolysaccharide (LPS), muramyl dipeptide (MDP) or recombinant interferon-γ (rIFNγ) for 2-48 h showed significant increases in the release of H2O2, O2̄ and interleukin-1 (IL-1) as compared to unrelated macrophages. The treatment of macrophages with different combinations of the above agents did not induce synergistic or additive effects on the production of H2O2, O2̄ and IL-1. The priming of macrophages with rIFN-γ had a significant effect in the additional increased production of H2Ō, O2- and IL-1 when subsequently treated with cisplatin, LPS or MDP.
Release of tumor cytolytic/cytostatic factor(s) by in vitro cisplatin treated macrophages: Enhanced tumoricidal activity by increase in osmotic fragility of target cells
(1991) S. Mahendra Singh; A. Sodhi; B. Geetha
[No abstract available]
Studies on chemiluminescence and protein kinase-c activity of cisplatin treated mouse peritoneal exudate cells
(Informa Healthcare, 1991) B. Geetha; Ajit Sodhi; Sukh Mahendra Singh
A single i.p. injection of cisplal-in (10 mg/kg body weight) into mice results in a significant increase in chemiluminescence and ATP contents of the peritoneal exudate cells (PEC) than that of PEC from untreated mice. It is also observed that in vitro treatment of macrophages with cisplatin, rIFN-γ and LPS show increased activity of the protein kinase-C.(PK-C). the activation of PK-C could result in stimulation of NADPH-oxidase resulting in increased levels of chemi luminescence. Increased contents of ATP in PEC after cisplatin treatment also suggests that this activation is energy dependent. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.