Browsing by Author "Chandra Kant Singh Tekam"

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Bilateral Common Carotid Artery Occlusion: Stroke Model
(Springer Singapore, 2021) Chandra Kant Singh Tekam; Saksha Shinde; Ranjana Patnaik; Sanjeev Kumar Mahto
Ischemic stroke is a prime spawn of death, physical disability and imparts an immense socioeconomic burden for society. Despite, rigorous experimental and clinical research work over the past few decades, still, therapeutic options are scarce for patients with acute ischemic stroke (AIS). It caused an increase in the percentage of ischemic patients in the ensuing years. One approach is to develop a better understanding of the brain’s cellular and molecular mechanisms to combat this harmful problem. One of the extensively used approaches for Brain-induced neuroprotection is ischemic preconditioning (IPC) or ischemic tolerance (IT). IPC is a non-harmful stimulus applied to the brain, which leads to interim resistance in the wake of ischemic insult. The IPC takes place in two different categories: initial IT, which lasts from a few minutes to a few hours after the IPC, and delayed IT, which takes a couple of hours to occur. Until now, the investigation has focused on delayed IT but the molecular mechanism of IT is largely unknown. This chapter aims to provide insight into Bilateral Common Carotid Aartery Occlusion (BCCAO) methodology and factors affecting the biological pathways in the course of neurodegeneration in rodents (mice and rats). © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021.
Effects of extremely low-frequency (50 Hz) electromagnetic fields on vital organs of adult Wistar rats and viability of mouse fibroblast cells
(Oxford University Press, 2025) Chandra Kant Singh Tekam; Shreyasi Majumdar; Pooja Kumari; Santosh Kumar Prajapati; Ajay Kumar Sahi; Richa S. Singh; Sairam Krishnamurthy; Sanjeev Kumar Mahto
In recent years, scientific communities have been concerned about the potential health effects of periodic electromagnetic field exposure (≤1 h/d). The objective of our study is to determine the impact of extremely low-frequency pulsed electromagnetic fields (ELF-PEMF) (1-3 mT, 50 Hz) on mouse fibroblast (red fluorescent protein (RFP)-L929) cells and adult Wistar rats to gain a comprehensive understanding of biological effects. We observed that RFP-L929 exhibits no significant changes in cell proliferation and morphology but mild elevation in aspartate aminotransferases, alanine aminotransferases, total bilirubin, serum creatinine, and creatine kinase-myocardial band levels in ELF-PEMF exposed groups under in vitro and in vivo conditions. However, the histological examination showed no significant alterations in tissue structure and morphologies. Our result suggests that 50-Hz ELF-PEMF exposure (1-3 mT, 50 Hz) with duration (<1 h/d) can trigger mild changes in biochemical parameters, but it is insufficient to induce any pathological alterations. © 2024 The Author(s). Published by Oxford University Press. All rights reserved.