Browsing by Author "Kumari Nishi"

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Genetic homogeneity appraisal of in-vitro plants regenerated from leaf explants of sapindus mukorossi using RAPD and ISSR molecular markers
(World Research Association, 2019) Singh Reetika; Akhtar Sabina; Kumari Nishi
Genetic fidelity is the main concern of the plant tissue culture. Two different DNA-based molecular markers, Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) were used for DNA fingerprinting of Sapindus mukorossi Gaertn. genotypes and to evaluate the genetic homogeneity between mother plant and in vitro raised plants. Total 32 primers (16 of RAPD and 16 of ISSR) were used for the generation of reproducible and scorable bands. The bands generated from all primers were clear, scorable and reproducible and bands range from 350-1250 bp in RAPD. Total 84 bands were produced with an average of 5.25 bands per primer. In ISSR, total 61 scorable bands were recorded with an average 3.81 bands per primer and bands range from 250-1250 base pairs. Results showed that in vitro raised plants were genetically similar to the mother plants. © 2019, World Research Association. All rights reserved.
Sapindus mukprossi Gaertn.: Rich source of antioxidants and reducing agents
(World Research Association, 2020) Singh Reetika; Kumari Nishi
Sapindus mukorossi Gaertn. is an important medicinal and economical tree. Our aim is to evaluate the antioxidant activity from various extracts (ethanolic, methanolic and aqueous) of callus and in vitro leaf extracts. Antioxidant activity and polyphenolic compound was evaluated through different standard methods. Correlation was calculated through linear regression analysis. Methanolic extract of in vitro leaf showed maximum antioxidant activity (602.28 μg ml-1), total flavonoid content (540.11±0.89 mg g-1 of rutin equivalent) and lipid peroxidation inhibition activity (11.70 μg ml-1). But total phenolic content was found maximum (338.76±0.72 mg g-1 of gallic acid equivalent) in ethanolic extract of in vitro leaf. Similarly, reducing potential was maximum in methanolic extract of callus. Very close and significant correlation was seen between antioxidant and polyphenolic content of in vitro leaf and callus extracts. Positive and significant correlation was also observed between antioxidant activities and reducing potential and indicates that reducers present in the extracts play an important role in antioxidant activity. As both extracts showed phytochemical activities, so they can be used for the production of natural antioxidants, pharmaceuticals and nutraceuticals. © 2020 World Research Association. All rights reserved.