Genetic homogeneity appraisal of in-vitro plants regenerated from leaf explants of sapindus mukorossi using RAPD and ISSR molecular markers

Abstract

Genetic fidelity is the main concern of the plant tissue culture. Two different DNA-based molecular markers, Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) were used for DNA fingerprinting of Sapindus mukorossi Gaertn. genotypes and to evaluate the genetic homogeneity between mother plant and in vitro raised plants. Total 32 primers (16 of RAPD and 16 of ISSR) were used for the generation of reproducible and scorable bands. The bands generated from all primers were clear, scorable and reproducible and bands range from 350-1250 bp in RAPD. Total 84 bands were produced with an average of 5.25 bands per primer. In ISSR, total 61 scorable bands were recorded with an average 3.81 bands per primer and bands range from 250-1250 base pairs. Results showed that in vitro raised plants were genetically similar to the mother plants. © 2019, World Research Association. All rights reserved.