Browsing by Author "M.N. Amin"

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In Vitro Propagation of Guava from Shoot Cultures of Mature Trees
(1987) V.S. Jaiswal; M.N. Amin
Studies on the in vitro propagation of Psidium guajava L. demonstrate that shoot tip explants from mature trees are capable of forming multiple shoots. Proliferation of shoot tip was obtained on MS basal medium containing different combinations and concentrations of auxins and cytokinins. However, highest number of shoots per explant was obtained from cultures grown on medium supplemented with 1 mg l-1 BAP only. Rooting was achieved by transferring the individual shoots to 1/2 strength MS medium having 0.1-0.5 mg 1-1 IBA and NAA. About 80% shoots rooted well when medium was supplemented with IBA and NAA (0.2 mg 1-1 each) together. Addition of activated charcoal showed beneficial effects on rooting percentages and plantlet growth. Plantlets were acclimated and successfully established in soil. © 1987, Gustav Fischer Verlag, Stuttgart. All rights reserved.
In vitro response of apical bud explants from mature trees of jackfruit (Artocarpus heterophyllus)
(Kluwer Academic Publishers, 1993) M.N. Amin; V.S. Jaiswal
A tissue culture technique for rapid vegetative propagation of mature jackfruit trees using apical bud cultures has been developed. Shoot-tip cultures were established on MS medium with 5-10 mm explants dissected from terminal buds of new growth from trunk. After initial culture of bud explants, one- to two-node pieces were taken from the microshoots formed and used to proliferate further axillary shoots for multiplying and maintaining shoot cultures. Benzyladenine and kinetin (4.5-9.0 μM), either separately or together, supported shoot proliferation; higher concentrations of the cytokinins inhibited bud breaking and favoured callus formation at the explant bases. Bud explants taken from emerging trunk sprouts invariably produced clumps of multiple shoots, whereas buds obtained from actively growing top branches generally elongated to form a solitary shoot. November to January was the best season for initiation of cultures from field-grown trees. Shoots proliferated at the initial subcultures had mature morphology and were difficult-to-root. Shoots assumed to be juvenile-like developed at the later passages and could be rooted with 60-80% success using 1/2-MS salts and 10 μM of indolebutyric acid or naphthaleneacetic acid. Regenerated plantlets were transferred to the soil and about 50% survived. © 1993 Kluwer Academic Publishers.