Browsing by Author "R. Maurya"

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A New Cucurbitacin Glucoside from Luffa graveolense
(Springer Science and Business Media, LLC, 2019) S. Kumar; K. Sharma; M. Sahai; R. Maurya
A new cucurbitacin glucoside has been isolated from an ethanolic extract of Luffa graveolense, along with four known cucurbitacins. Its structure has been assigned as 20-O-β-D-glucopyranosyl cucurbitacin B (2) by spectroscopic analysis. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
Biointensive Integrated Pest Management of Chilli
(Jenny Stanford Publishing, 2021) R.R. Dhole; S.P. Patil; R.M. Mahalle; R. Maurya
[No abstract available]
Biointensive Integrated Pest Management of Tomato
(Jenny Stanford Publishing, 2021) R.R. Dhole; S.P. Patil; R. Maurya; R.N. Singh
[No abstract available]
Comparative evaluation of parasitology and serological tests in the diagnosis of visceral leishmaniasis in India: A phase III diagnostic accuracy study
(2007) S. Sundar; R.K. Singh; S.K. Bimal; K. Gidwani; A. Mishra; R. Maurya; S.K. Singh; K.D. Manandhar; M. Boelaert; M. Rai
In this phase III trial for diagnostics for visceral leishmaniasis (VL) in India, we compared parasitological diagnosis with several serological tests: direct agglutination test (freeze dried; DAT-FD), rK-39 strip test, rK-26 strip test and a latex agglutination test for antigen detection in urine (KAtex) in 452 subjects from the endemic regions of Bihar, India. The subjects were segregated into four categories: 230 confirmed patients, 52 probable cases, 70 non-cases and 100 healthy endemic controls. The first two groups were used for estimating sensitivity, the latter two for specificity. Sensitivity of DAT-FD was 98.9%, rK-39: 98.9%, KAtex: 67.0% and rK-26: 21.3%. Sensitivity of DAT-FD on blood taken on filter paper (DAT-FDF) was 99.3%, which was comparable with that using serum. Specificity of serological tests was comparable and high (DAT-FD and DAT-FDF: 94%, rK-39 strip test: 97%, KAtex: 99% and rK-26 strip test: 100%). The classical 'gold standard' parasitological demonstration in splenic smear performed poorly as it missed 18.4% of cases that benefited from VL treatment. Reproducibility of the serological tests between field and central laboratories was excellent (κ = 1.0, 0.99, 0.96 and 0.94 respectively for microscopy, DAT-FD, rK-39 strip test and rK-26 strip test). A high degree of agreement was observed between DAT-FD and rK-39 strip test (κ = 0.986). Although DAT-FD and rK-39 strip test were highly sensitive with excellent specificity, the ease of use of the latter makes it most suitable for the diagnosis of VL in the field conditions. © 2007 Blackwell Publishing Ltd.
Constituents of Pterocarpus Marsupium
(1984) R. Maurya; A.B. Ray; F.K. Duah; D.J. Slatkin; P.L. Schiff
[No abstract available]
Evaluation of PCR for diagnosis of Indian kala-azar and assessment of cure
(2005) R. Maurya; R.K. Singh; B. Kumar; P. Salotra; M. Rai; S. Sundar
This study was done to evaluate PCR with Ld1 primers for the diagnosis of Indian visceral leishmaniasis (VL) and to assess its role in prediction of the disease outcome. The PCR assay was performed with DNA isolated from the peripheral blood of parasitologically confirmed cases of VL before the initiation of treatment, just after the end of treatment, and at 3 and 6 months of follow-up. The pretreatment PCR result was positive for 100 of 101 patients (sensitivity, 99%; confidence interval [CI], 94 to 100%). None of the 150 negative controls tested were PCR positive (specificity, 100%; CI, 96.8 to 100%). Of 60 patients who were treated at our center, 51 (85%; CI, 73 to 93%) became negative immediately after treatment and continued to be negative at 3 and 6 months of follow-up. At the 3-month follow-up, two of the remaining nine patients were PCR positive, making 58 (96.7%; CI, 87 to 100%) patients PCR negative. At the 6-month follow-up, all patients became PCR negative. One patient who was PCR negative immediately after the end of treatment relapsed 11 months later. This limited prospective study with VL patients suggests that the PCR assay is a highly sensitive and specific (99% and 100%, respectively) tool for the diagnosis of VL. In the majority of patients, it can identify a successful disease outcome; however, its translation into the field setting remains a major challenge.
Human visceral leishmaniasis is not associated with expansion or accumulation of Foxp3+ CD4 cells in blood or spleen
(Blackwell Publishing Ltd, 2010) R. Maurya; R. Kumar; V.K. Prajapati; K.D. Manandhar; D. Sacks; S. Sundar; Susanne Nylén
Natural regulatory T cells (CD4+ CD25+ Foxp3 +), natural regulatory T cells (nTreg), play an important role in the regulation of inflammatory immune responses. However, the immunosuppressive properties of nTreg may unfavourably affect the host's ability to clear certain infections. In human visceral leishmaniasis (VL), reports on the frequency and function of nTreg are not conclusive. A limitation of our own previous studies that did not indicate a major role for Foxp3+ nTreg in VL pathogenesis was that Foxp3 was measured by mRNA expression alone, as other tools were not available at the time. We have in this study assessed CD4 +CD25+Foxp3+ cells in splenic aspirates and peripheral blood mononuclear cells (PBMC) from an extensive series of patients with VL and endemic controls (EC) by flow cytometry (FACS). The results do not show increased frequencies of Foxp3+ cells in patient with VL pre-and post-treatment, neither were they elevated when compared to PBMC of EC. We conclude that active VL is not associated with increased frequencies of peripheral Foxp3 Treg or accumulation at the site of infection. © 2010 Blackwell Publishing Ltd.
Serological diagnosis of Indian visceral leishmaniasis: direct agglutination test versus rK39 strip test
(2006) S. Sundar; R.K. Singh; R. Maurya; B. Kumar; A. Chhabra; V. Singh; M. Rai
We evaluated the direct agglutination test (DAT), using freeze-dried (FD) and aqueous (AQ) antigen, and the rK39 immunochromatographic strip test in the diagnosis of Indian visceral leishmaniasis (VL). Sera from 508 subjects (150 parasitologically confirmed patients with VL, 100 and 153 healthy controls drawn from non-endemic and endemic regions, respectively, and 105 patients with other diseases presenting with fever and/or splenomegaly) were tested. The sensitivity of the tests were as follows: DAT (FD), 96% (95% CI 91-98); DAT (AQ), 97% (95% CI 93-99); rK39 strip test, 99% (95% CI 95-100). The specificity of DAT (FD), DAT (AQ) and rK39 strip tests were 85% (95% CI 81-88), 87% (95% CI 83-91) and 89% (95% CI 86-92), respectively. A significant correlation (high degree of agreement) was observed between all tests (κ > 0.80). We conclude that the sensitivity of FD antigen is comparable to that of AQ antigen. Similarly, the rK39 strip test is as sensitive as the DAT, but the strip test's greater convenience of use makes it a better tool for diagnosis of VL in peripheral areas of endemic regions. © 2005 Royal Society of Tropical Medicine and Hygiene.
The synthesis of propterol, a novel 1,3-diarylpropan-2-ol from pterocarpus marsupium
(1985) R. Maurya; A.B. Ray; S.K. Chattopadhyay; F.K. Duah; M.C. Lin; D.J. Slatkin; P.L. Schiff
[No abstract available]