Browsing by Author "S. Majumder"

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Anti-bacterial Role of Zinc Oxide Synthesized Using Naturally Abundant Material
(John Wiley and Sons Inc, 2025) Mansi; Shivank; Bhaskar Bhattacharya; S. Majumder; Jahanvi Kashyap; Yatishwar Chauhan
The large-scale chemical synthesis of nanoparticles has a very detrimental effect on the environment. Therefore, w e explored a green route for the synthesis of ZnO nanoparticles using plant extract. W e report the synthesize of ZnO nanoparticles via both green and chemical routes for comparison. Both samples are characterized using a UV-visible spectrophotometer, X-ray diffraction analysis (XRD), and a photo-illuminator. Their properties are compared, and then their antimicrobial activity is tested. Notably, the green synthesis method is cost-effective and eco-friendly compared to chemical synthesis. © 2025 Wiley-VCH GmbH.
Impedimetric detection of Banana bunchy top virus using CdSe quantum dots for signal amplification
(Springer Nature, 2020) S. Majumder; Bhaskar Bhattacharya; Pramod K. Singh; Shivangi Johari; Bharat Singh; Razia Rahman
Banana bunchy top virus is considered to be the most economically destructive pathogens of banana that causes severe economic loss in banana plantations worldwide, including India. In this present study we have developed an improved electrochemical ELISA for detection of Banana bunchy top virus (BBTV). For enhanced and accurate detection we have used cadmium selenide (CdSe) quantum dots (QDs) as signal amplifiers. Experiments in this study were performed using primary antibody raised from recombinant coat protein of BBTV. CdSe QDs could significantly amplify the electrical signals in this assay and make the method appropriate for lab use. The result of electrical conduction showed the difference of impedance between the healthy and diseased sample of the order of ~ 100 Ω. The electrochemical ELISA could detect the virus in plant sap up to dilution of 1:25 as compared to 1:10 of conventional ELISA. © 2020, Springer Nature Switzerland AG.
Reversal of drug resistance in P-glycoprotein-expressing T-cell acute lymphoblastic CEM leukemia cells by copper N-(2-hydroxy acetophenone) glycinate and oxalyl bis (N-phenyl) hydroxamic acid
(2006) S. Majumder; P. Dutta; P. Mukherjee; E.R. Datta; T. Efferth; S. Bhattacharya; S.K. Choudhuri
Multiple drug resistance (MDR) represents a major obstacle to successful application of chemotherapy and a basic problem in cancer biology. MDR occurs at the cellular level and is multi-factorial in nature. The multidrug resistance gene, MDR1, and its gene product P-glycoprotein (P-gp) are now well known as an important determinant of MDR. Much effort has been devoted to develop P-gp inhibitors to modulate resistance. However, most of these resistance-modifying agents (RMA) are too toxic at the required doses. Therefore, the development of novel RMAs to overcome MDR represents a major challenge to modern cancer chemotherapy. In the present investigation, we describe the effect of oxalyl bis (N-phenyl) hydroxamic acid (OBPHA) and copper N-(2-hydroxy acetophenone) glycinate (CuNG) on multidrug-resistant P-gp-expressing CEM/ADR5000 T-cell acute lymphoblastic leukemia cells. CuNG, a known depleting agent for glutathione (GSH) and inhibitor of glutathione S-transferase (GST) and multidrug resistance-related protein 1 (MRP1), also inhibited P-gp-mediated doxorubicin accumulation and retention. The resistance-modifying effects of OBPHA were stronger than that of CuNG. Both novel RMAs overcame drug resistance more efficiently than verapamil, a well-known P-gp inhibitor. OBPHA and CuNG exposure resulted in an increased doxorubicin accumulation after 1-3 h incubation by down-regulation of P-gp expression after 24 h incubation. This is a clue that different mechanisms may contribute to modulation of P-gp-mediated drug resistance by these compounds. © 2005 Elsevier Ireland Ltd. All rights reserved.