Impedimetric detection of Banana bunchy top virus using CdSe quantum dots for signal amplification

Abstract

Banana bunchy top virus is considered to be the most economically destructive pathogens of banana that causes severe economic loss in banana plantations worldwide, including India. In this present study we have developed an improved electrochemical ELISA for detection of Banana bunchy top virus (BBTV). For enhanced and accurate detection we have used cadmium selenide (CdSe) quantum dots (QDs) as signal amplifiers. Experiments in this study were performed using primary antibody raised from recombinant coat protein of BBTV. CdSe QDs could significantly amplify the electrical signals in this assay and make the method appropriate for lab use. The result of electrical conduction showed the difference of impedance between the healthy and diseased sample of the order of ~ 100 Ω. The electrochemical ELISA could detect the virus in plant sap up to dilution of 1:25 as compared to 1:10 of conventional ELISA. © 2020, Springer Nature Switzerland AG.