Browsing by Author "Sanjeev Singh"

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Influence of ferric chloride and humic acid on bentonite as clay liner
(Maney Publishing, 2010) Sanjeev Singh; Arun Prasad
The bentonite-based material is well established in several countries as potential barriers and a seal for waste disposal system. The properties of bentonite vary widely depending on its origin. Bentonite soil is often used as a material for clay liner. But its engineering properties change when it comes in contact with different chemicals. In order to investigate whether bentonite available locally in India could be useful as a buffer or sealing material in an MSW/HLW repository system. Bentonite samples added with different chemicals were examined in terms of their physico-chemical properties such as surface area, cation exchange capacity, swelling rate etc. To study the effect of chemicals (both inorganic and organic) on bentonite, two chemicals (Ferric Chloride and Humic acid) were selected that are generally found in municipal solid waste. The effect of these chemicals on Bentonite soil has been studied in a controlled condition in the laboratory. The optimum values of these chemicals to be added, are evaluated and added separately to bentonite soil. The engineering properties such as differential free swell, hydraulic conductivity and swelling pressure were found out. Tests were also carried out to evaluate shear strength parameters of bentonite soil upon contamination with chemicals. The selected soil is considered to be highly expansive in nature. In addition to these tests some more tests were also carried out to study the fabric of soil such as scanning electron microscopy (SEM), cation exchange capacity (CEC), infra-red spectroscopy (IR), x-ray diffraction (XRD) and specific surface area (SSA). J. Ross Publishing, Inc. © 2010
Isolation and characterization of antibiotic and antimetabolite resistant mutants of Rhizobium sp. and their symbiotic interaction with Sesbania aculeata L.
(2009) V.P. Singh; S. Kumar; Sanjeev Singh
A native rhizobial strain {Rhizobium sp.) nodulating dhaincha {Sesbania aculeata L.) was isolated, and designated as RS-02. The sensitivity of RS-02 strain was studied against two antibiotics (Streptomycin and Rifampicin) and one antimetabolite (Sodium azide). Spontaneously occurring antibiotic / antimetabolite resistant mutants were isolated and designated as Str, Rif and Azi respectively. These resistant mutants were examined for their physiological characteristics (carbon and nitrogen utilization), cross resistance to each other and symbiosis with the host plant dhaincha. The parent strain along with resistant mutants were tested for their nodulation and symbiotic nitrogen fixation traits. It was found that all the mutant strains were able to infect dhaincha roots. The effectiveness of the marked rhizobial mutants in dhaincha exhibited a significant difference in symbiotic traits.
Mutagenic effects of gamma rays and EMS IN M1 and M2 generations in two traditional genotypes of aromatic rice (oryza Sativa L.)
(DAV College, 2017) Satish Kumar. Chakravarti; Sanjeev Singh; C.N. Ram; M.K. Vishwakarma; Gauri Shankar Verma
With the aim to know immediate effect of mutagenic treatment in two traditional non Basmati rice varieties viz. Kalanamak and Badshah Bhog, in terms of biological damage. We examined reduction in seeds germination, root and shoot length, plant survival at maturity, pollen fertility and grain yield in M1 generation. Most of the mutagenic treatments severely affected the genotypes, Kalanamak and Badshah Bhog both. A dose dependent increased in biological damage was observed in most of the mutagenic treatment and maximum biological damaged was caused by 40 kR gamma rays + EMS (0.2%) followed by 30kR gamma rays+EMS(0.2%) treatment, while minimum at 10kR gamma rays. Biological damage was relatively more in Badshah Bhog as compared to genotype Kalanamak. A linear relationship was observed between doses of gamma ray and damage in different biological parameters, such as, plant survival at maturity, root and shoot growth, pollen fertility as well as grain yield. The mutagenic efficiency was found maximum at 0.2% EMS in Kalanamak and Badshah Bhog and the mutagenic effectiveness was found maximum at 10 kR gamma rays in both the varieties Kalanamak and Badshah Bhog.
Response of potato (Solanum tuberosum l.) to irrigation methods, moisture regimes and integrated nitrogen management
(Indian Society of Agronomy, 2021) Sanjeev Singh; Bhoop Narayan Singh; Adesh Singh; Rakesh Chandra Tiwari; Mohammad Hasanain; Tejbal Singh; Anand Singh; Yakshi Agrawal
A field experiment was conducted in split-plot design during the winter (rabi) season of 2015–16 at Agronomy Research Farm, Kumarganj, Ayodhya (Uttar Pradesh) to evaluate the response of potato (Solanum tubersum L.) to irrigation methods, moisture regimes and nitrogen management. Treatments effect was non-significant on initial plant stand [15 days after planting (DAP)]. The plant height, number of haulms, number of leaves at 90 DAP and dry weight of haulms were significantly superior under regular furrow irrigation method, 1.0 irrigation water: cumulative pan evaporation (IW: CPE) and 75% dose of urea through N + 25% dose of N through FYM over their counterparts. All the growth characters were found highest under nitrogen-management treatment 75% dose of urea through N + 25% dose of N through FYM, which was statistically superior to rest of the nitrogen-management treatments. Yield attributes, viz. number of tubers (grade-wise)/hill, weight of tubers (grade-wise) (kg/plot), and tuber yield (q/ha) were found highest under regular furrow method of irrigation (M1 ) and moisture regime, 1.0 IW: CPE (I2 ) over 0.8 IW: CPE (I1 ) and 1.2 IW: CPE (I3 ). Moisture regimes significantly influenced number of all grade of tubers. All the yield attributes were significantly higher under nitrogen management treatment N2, and was statistically superior to rest of the nitrogen management treatments. © 2021, Indian Society of Agronomy. All rights reserved.
Screening of Brassica rapa L. var. yellow sarson genotypes against downy mildew Alternaria blight
(Springer India, 2014) Kamlesh Kumar Prajapati; O.P. Verma; Prakash Singh; Sanjeev Singh; Dhirendra K. Singh
The use of resistant varieties is considered to be the best method of disease control. Therefore, the study was carried out to find out the sources of resistance against downy mildew (caused by Peronospora brassicae (Pers. ex. Fr.)) and Alternaria blight (Alternaria brassicae (Berk.) Sacc. and Alternaria brassicicola (Schw.) Wiltshire) in Brassica rapa. The pathogen is greatly influenced by weather with the highest disease incidence in wet seasons and in areas with relatively high rainfall. A. brassicae can affect host species at all stages of growth, including seed. Symptoms of the disease are characterized by formation of spots on leaves, stem and siliquae. This investigation was conducted under three different environments in randomized block design with three replications. A total of 20 genotypes of yellow sarson were screened for varietal reactions against the diseases under field conditions during rabi 2010 and 2011. None of the genotypes was found free from both the disease, in case of downy mildew disease; only one genotype named Jagrati was found moderately resistant. For Alternaria blight, seven genotypes named, Benoy, Pusa Gold, YSK 09–1, PYS 2008–2, NRCYS-05–02, YSWB-2012 and NDYS-427 were found moderately resistant. © Springer India 2014 This work is subject. All rights reserved.
Studies on genetic parameters in induced micro-mutation population of aromatic rice (Oryza Sativa L.) in M2 and M3 generations
(DAV College, 2014) Satish Kr. Chakravarti; Sanjeev Singh; H. Kumar
An attempt has been made to study the genetic parameters of rice to develop the selection criteria for improving the traits under study. In general, combined treatments showed higher magnitude of heritability and genetic advance as compared to single treatments in both the generations in both the aromatic rice genotypes. A large number of promising families were isolated from M2 population, most of them performed well in M3 generation. The values of GCV, PCV heritability (in broad sense) and genetic advance were high for most of the traits in both the varieties in the combination treatment of 40kR gamma-ray + EMS in both the generations.