Effect of spraying zinc element fertilizer on the growth and yield of green pea (Pisum sativum L.) seeds

Abstract

Medicinal plant-endophytic bacteria interactions modulated production of secondary metabolites finds wide range of application in agriculture in growth promotion and stress tolerance, medicines and industries. Endophytic bacteria were isolated from Costus igneus (insulin plant; (IP) from roots and leaves (L) explants, using King’s B (K) and NMS (N) media. Based on the biochemical characteristics determined by Biolog GEN III and its extensive species library isolates were identified as Terrimonas ferruginea (IPNR), Massilia lutea (IPKR), Klebsiella variicola (IPNL) and Raoultella terrigena (IPKL). All the four isolates showed the production of indole acetic acid (IAA) which was high in root endophytic isolates. The siderophore production was strain specific characteristics. The strains were positive to catalase activity. All the endophytic bacteria isolate solubilized tricalcium phosphate and chitin. Solubilization of insoluble potash (mica) and zinc (ZnO2) and was more in root isolates. The isolates were sensitive to streptomycine, kanamycine, tetracycline and resistant to ampicillin and nalidixic acid. Among the isolates IPKR exhibited maximum production of IAA and siderophore and P, K, Zn and chitin solubilization. © 2020 Plant Archives. All rights reserved.