Salt tolerant callus lines of Dendrocalamus strictus nees under salt stress: Growth and ion accumulation

Abstract

Plant tissue culture technique offers a suitable opportunity for development of salt tolerant plants. The technique is also helpful in understanding physiological and molecular mechanisms of tolerance to salt stress under In vitro condition at the cellular, organ, and whole-plant level. This study was performed to understand the growth responses and accumulation of Na+ and K+ ions against the salt stress in NaCl tolerant callus of Dendrocalamus strictus Nees. The embryogenic callus tolerant to 100mM NaCl was screened out by exposing to increasing (0-200mM) concentrations of NaCl in 0.8% (w/v) agar gelled Murashige and Skoog medium having 3% (w/v) sucrose, 13.6 µM 2,4-Dichlorophenoxy acetic acid and 2.3µM Kinetin (Callus initiation medium). Stable growth of NaCl tolerant callus lines on NaCl-free as well as on NaCl-containing medium was recorded. The relative growth rate of NaCl sensitive callus decreased significantly with increasing concentrations of NaCl into the medium while, for tolerant callus was consistent on medium having 50 and 100mM NaCl and decreased further with increasing concentration of NaCl. The FW/DW of NaCl sensitive callus was higher than the NaCl- tolerant callus. The ratio K+/Na+ was recorded low when the callus was grown on medium supplemented with NaCl and among sensitive and tolerant callus this ratio was found least in tolerant callus when they were grown on medium having 100mM NaCl. © 2019 Plant Archives. All rights reserved.