Activation of murine macrophages by tumor necrosis factor, interleukin-1, interferon-γ and cisplatin

Abstract

Murine peritoneal macrophages were rendered tumoricidal to Dalton's lymphoma (DL) cells on incubation with recombinant tumor necrosis factor alpha (rTNF-α), recombinant interleukin-1 (rIL-1) and cisplatin in vitro. Simultaneous treatment of macrophages with suboptimal doses of rTNF-α and rIL-1 had additive effect on the activation of macrophages. Priming of macrophages with recombinant interferon gamma (rIFN-γ) significantly enhanced the rTNF-α and rIL-1-induced macrophage cytotoxicity. Cisplatin was found to up-regulate rIL-1-induced macrophage activation but inhibited the activation of macrophages with rTNF-α. These studies indicate the potential of appropriate combination of these Biological Response Modifiers (BRMs) against neoplasia. © 1990.