Rapid detection of enterotoxigenic Escherichia coli gene using bio-conjugated gold nano-particles

Abstract

In this study, a simple gold nanoparticle based colorimetric detection system for on site diagnostics of Enterotoxigenic Escherichia coli (ETEC) has been developed. The developed assay could detect LT1 gene as evident from UV-visible spectra, the broadening of peak on hybridization of GNP probes with different copy numbers of the target gene.The present study suggests that hybridization induced changes in optical properties of GNPs can be translated into a rapid and simple colorometric" spot and read test detection method for ETEC. Copyright © 2011 American Scientific Publishers All rights reserved.