Browsing by Author "Dash, Manoj Kumar"

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    Ayurvedic preparations of Raudra Rasa inhibit agonist-mediated platelet activation and restrict thrombogenicity without affecting cell viability
    (John Wiley and Sons Inc, 2023) Chaurasia, Susheel Nidhi; Singh, Vipin; Ekhlak, Mohammad; Dash, Manoj Kumar; Joshi, Namrata; Dash, Debabrata
    Ayurveda is considered to be one of the most ancient forms of medicine still practiced. The Ayurvedic preparation Raudra Rasa and its derivatives have been widely employed against cancer since the 12th century, but the effect of these traditional formulations on platelet function and signaling has not previously been examined. Here we demonstrate that Raudra Rasa and its derivatives significantly reduce thrombin-induced integrin activation and granule secretion in platelets, as observed by reduced PAC-1 binding and P-selectin externalization, respectively. These formulations also inhibited thrombin-stimulated phosphatidylserine exposure, mitochondrial reactive oxygen species generation, and mitochondrial transmembrane potential in platelets. Consistent with the above, Raudra Rasa significantly reduced thrombin-induced tyrosine phosphorylation of the platelet proteins, as well as phosphorylation of the enzymes AKT and GSK-3?. In summary, Raudra Rasa inhibits agonist-mediated platelet activation without affecting cell viability, suggesting it may have therapeutic potential as an anti-platelet/anti-thrombotic agent. � 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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    Ayurvedic supportive therapy in the management of breast cancer
    (Elsevier GmbH, 2021) Dash, Manoj Kumar; Joshi, Namrata; Gautam, D.N.S; Jayakumar, Remya; Tripathi, Y.B.
    Introduction: Breast cancer is the second most common cancer among women. Initial signs of cancer start in the lymph nodes located under the arm, within the breast, and near the collarbone. Many promising remedies for breast cancer are included in Ayurveda, the Indian system of medicine. Nowadays, multi-targeted herbal drugs are used as an adjuvant therapy alongside conventional medicine in the management of t0068is type of cancer. The present review concentrates on the available literature found in Ayurveda regarding plants and breast cancer. Methods: Web of Science, PubMed, Google Scholar were searched, resulting in 115 articles, as per the authors study criteria, by using keywords, breast cancer, and medicinal plants, then were able to aggregate 64 therapeutic targets from 35 different plant species in this review article. Result: Seven treatment areas were identified: Anti-inflammatory, Immunomodulatory, Endocrine suppressor, Tumour suppressor, Glut-1 inhibitor, Aromatase inhibitor, and Lectin-containing plants. Among the molecular targets, 51 % (33/64) caused apoptosis and 37 % (24/64) demonstrated cell proliferation activity. The review provides evidence of data suggesting that 17 % therapeutic targets have carcinogen inactivation activity, 10 % have precancerous growth prevention activity, 9% have mutagenesis and gene expression activity, and 4 % regulate toxic metabolism. Conclusion: Ayurvedic herbs that suppress multiple pathways must be chosen for prevention as well as treatment of the disease. It may not only be beneficial in reducing the harms of chemotherapeutic agents but also enhances their efficacy as supportive care. � 2021 Elsevier GmbH
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    Physico-chemical characterization of kajjali, black sulphide of mercury, with respect to the role of sulfur in its formation and structure
    (Elsevier B.V., 2021) Joshi, Namrata; Dash, Manoj Kumar; Upadhyay, Chandan; Jindal, Vikas; Panda, Pradip Kumar; Shukla, Manjari
    Background: Kajjali is used as a base for Ayurvedic herbo-mineral medicines. It is a combination of mercury with sulfur in varying proportions. The ratio of sulfur (S) added to mercury (Hg) directly relates to the therapeutic efficacy of the compound. Objective: To analyze the physico-chemical characteristics of samaguna gandhaka kajjali (Hg: S = 1:1) and shadaguna gandhaka kajjali (Hg: S = 1:6). Materials and methods: X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy (XPS), Fourier transmission infrared spectroscopy, thermo-gravimetry analysis, and atomic absorption spectroscopy were applied to characterize each type of kajjali. Results: It was found that the particle size of the formed kajjali compound increases with a decrease in the mercury to sulfur ratio. The presence of excess sulfur does not change the surface oxidation states as revealed by the XPS analysis. No trace of mercury has been found in both samaguna gandhaka kajjali (SGK-1) and shadguna gandhaka kajjali (SGK-6), indicating a complete Hg reaction with S. Conclusion: Kajjali simulates nanomaterial of the modern era and possesses therapeutic efficacy as mentioned in classical Ayurveda texts. Complete trituration of mercury and sulfur combination ends up with this kajjali formation incorporating the potency of nanotherapeutics. � 2021 The Authors
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    Screening of anti-cancerous potential of classical Raudra rasa and modified Raudra rasa modified with hiraka bhasma (nanodiamond) through FTIR & LC-MS analysis
    (De Gruyter Open Ltd, 2022) Dash, Manoj Kumar; Joshi, Namrata; Dubey, Vd. Sushil; Dwivedi, Kamal Nayan; Gautam, Dev Nath Singh
    Objectives: Raudra rasa is an ayurvedic medicine explicitly prescribed for the treatment of arbuda (cancer), whereas hiraka bhasma has the potential to promote cancer healing properties. Together, these two medicines provide multifunction benefits. This paper analyses the functional groups of Raudra rasa modified with hiraka bhasma and compares it with the classically prepared raudra rasa. To identify the functional group, organic ligands, and active compounds present in samples of raudra rasa (CRR) and modified raudra rasa with hiraka bhasma (MRR) contributing to cancer alleviation by using Fourier transform infrared spectroscopy (FTIR) & LC-MS analysis. Methods: Classical raudra rasa (CRR), its ingredients, shadguna kajjali (SK); decoction of Piper betel Linn. (PBD); Amaranthus spinosus Linn. (ASD); Boerhaavia diffusa Linn. (BDD); Piper longum Linn. (PLD); cow urine (GM), & similarly modified raudra rasa (MRR), its ingredients, hiraka bhasma (HB); shadguna rasasindura (SHR); water-soluble extract of Piper betel Linn. (PBE); Amaranthus spinosus Linn. (ASE); Boerhaavia diffusa Linn. (BDE); cow urine ark (GA); Piper Longum Linn. (PLE) were subjected to FTIR and LC-MS analysis. Results: Among all 15 samples studied, maximum numbers of peaks (21) were seen in MRR indicating a greater number of functional groups. Further, in MRR, a maximum peak in the double bond region is suggestive of its higher stability compared to CRR. Both the compound is preliminarily a mixture of the number of functional groups like; fluoro, methyl, amino, hydroxy, nitro, methylamino, carbonyl, and iodo groups, having known anti-proliferative activities. By the FT-IR analysis, the biologically active compounds in aqueous and methanol extract of CRR & MRR were identified that have anti-cancerous compounds. In the present study, a total of 40 major compounds like alkaloids, amino acid, carboxylic acid, Flavonoids, Nucleoside, Nucleotide, phenylpropanoid, Sphingosine, stilbenoid, sugar, phosphate, terpenoids, vitamin from aqueous & methanol extract of CRR & MRR were identified by LC-MS. Conclusions: This research paper highlights the presence of different functional groups and bioactive compounds known to have anti-cancer activities. Thus, this review suggests future recommendations for the design and development of improved anticancer drugs with higher efficacy. � 2022 Walter de Gruyter GmbH, Berlin/Boston.
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    The Potential Impact of Ayurvedic Traditional Bhasma on SARS-CoV-2-Induced Pathogenesis
    (Bentham Science Publishers, 2023) Kumar, Pankaj; Jayakumar, Remya; Dash, Manoj Kumar; Joshi, Namrata
    The mass casualties caused by the delta variant and the wave of the newer �Omicron� variant of SARS-COV-2 in India have brought about great concern among healthcare officials. The government and healthcare agencies are seeking effective strategies to counter the pandemic. The application of nanotechnology and repurposing of drugs are reported as promising approaches in the management of COVID-19 disease. It has also immensely boomed the search for productive, re-liable, cost-effective, and bio-assimilable alternative solutions. Since ancient times, the traditional-ly employed Ayurvedic bhasmas have been used for diverse infectious diseases, which are now employed as nanomedicine that could be applied for managing COVID-19-related health anomalies. Like currently engineered metal nanoparticles (NPs), the bhasma nanoparticles (BNPs) are also packed with unique physicochemical properties, including multi-elemental nanocrystalline compo-sition, size, shape, dissolution, surface charge, hydrophobicity, and multi-pathway regulatory as well as modulatory effects. Because of these conformational and configurational-based physico-chemical advantages, Bhasma NPs may have promising potential to manage the COVID-19 pandemic and reduce the incidence of pneumonia-like common lung infections in children as well as age-related inflammatory diseases via immunomodulatory, anti-inflammatory, antiviral, and adju-vant-related properties. � 2023 Bentham Science Publishers.
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    Transmission modes of COVID-19 disease pandemic in the light of ancient wisdom of Ayurveda medicine: a review
    (De Gruyter Open Ltd, 2022) Joshi, Namrata; Dash, Manoj Kumar; Jayakumar, Remya
    Human-to-human transmission of the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurs most often when people are in the incubation stage of the disease or are carriers and have no symptoms. SARS-CoV-2 transmission on different levels showed that the cold and dry climate, hot wind and relative humid wind, maximum wind speed, turbulent wind, intensified recirculating flow were causing transmission higher than factors. The role of environmental factors described in Ayurveda like air, water, soil, season, frequent earthquakes, sunlight exposure, frequent thunderstorm with lightening, and factors from arthropods were revalidated in perspective of COVID-19 transmission. The authors searched the information regarding epidemic diseases in Ayurveda textbooks. Pubmed, Science Direct, Wikipedia, Elsevier, Lancet, and Springer were approached for the latest knowledge relating to SARS-CoV-2 and COVID-19. Google Scholar search engine was thoroughly checked upon for scientific evidence regarding the Ayurveda drugs. Various environmental factors like soil, air, water, frequent earthquake disasters, wildlife animals, aquatic birds, space, inevitable disastrous factors, weather or seasonal hazards, violent thunderstorm with lightning, intermediate hosts, sunlight exposure etc. were considered for their role in the genesis of the disease. The Ayurvedic concepts behind the etiology and development of epidemics are the same as modern epidemiological theories. The mysteries of many aspects of the current pandemic might be deciphered by traditional medicine knowledge and thus adding upon to the integrative medicine concept. � 2021 Walter de Gruyter GmbH, Berlin/Boston.