Browsing by Author "Garg, Pooja"

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    PublicationReview
    CRISPR/Cas9: efficient and emerging scope for Brassica crop improvement
    (Springer Science and Business Media Deutschland GmbH, 2025) Sharma, Shiv Shankar; Pandey, Ashwani; Kashyap, Anamika; Goyal, Lakshay; Garg, Pooja; Kushwaha, Ranjeet; Sharma, Jyoti; Tripathi, Shikha; Kumari, Sujata; Thomas, George; Verma, Malkhey; Gupta, Navin C.; Gupta, Ashish Kumar; Bhattacharya, Ramcharan; Sharma, Sandhya; Rao, Mahesh
    Main conclusion: CRISPR/Cas9 revolutionizes Brassica crop improvement by enhancing yield, quality, and stress resistance, providing a precise and versatile tool for genetic and agronomic advancements. Abstract: The rapidly advancing CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) technologies are being employed in both diploid and polyploid species of Brassica for gene functions and precise genetic improvements. CRISPR/Cas technology has sparked significant attention among the scientific community due to its affordability, precision, and effectiveness compared to other genome editing techniques. The recent discoveries highlight the diverse applications of the CRISPR/Cas9 genome editing tool in enhancing agriculturally important traits in Brassica species. This technology has been utilized to improve yield, quality, and resistance to both biotic and abiotic stresses globally. Here, we present an overview that encourages researchers to explore and improve the functionality and genetic progress of Brassica U-triangle species utilizing genome editing technologies. In addition, ethical considerations and concerns associated with CRISPR technologies are addressed, providing valuable insight into how CRISPR/Cas9 tools and have revolutionized crop improvement with special emphasis on Brassica for various agronomically and nutritionally important traits. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2025.
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    PublicationArticle
    Insights into early generation synthetic amphidiploid Brassica juncea: a strategy to harness maximum parental genomic diversity for improving Indian mustard
    (Frontiers Media SA, 2025) Garg, Pooja; Tripathi, Shikha; Kashyap, Anamika; Anil Kumar, A.; Kumari, Sujata; Singh, Mandeep; Kushwaha, Ranjeet; Sharma, Shiv Shankar; Sharma, Jyoti; Yadav, Rashmi; Gupta, N.C.; Singh, Naveen; Bhattacharya, Ramcharan; Chhokar, Vinod; Rao, Mahesh
    In India, amphidiploid Brassica juncea (AABB, 2n=36) is a significant oilseed crop, but its small gene pool limits its ability to develop traits of higher breeding and economic value. Through interspecific hybridization from various lines of the progenitor species, resynthesized B. juncea (RBJ) can provide breeders with additional resources for creating genetically diverse stress-tolerant and high-yielding cultivars. Three B. rapa accessions and eight B. nigra accessions were crossed in this study to develop 33 synthetic B. juncea lines. A total of 28 crosses were attempted, including the three-way crosses, but only the cross combinations with B. rapa cytoplasm led to successful embryonic development. Molecular diversity analysis of these lines in S2 generation revealed significant genetic diversity with higher levels of heterozygosity and allelic richness, along with significant variations for the yield-related traits. These results suggest that the synthesized lines could effectively enrich the genetic base of B. juncea and generate variability for agronomically important traits in a shorter time duration. The characterized variability in the synthetic lines needs to be utilized in hybridization, with already evolved genotypes, in early generations before it is lost due to chromosomal rearrangements, recombination and natural selection. Copyright © 2025 Garg, Tripathi, Kashyap, Anil Kumar, Kumari, Singh, Kushwaha, Sharma, Sharma, Yadav, Gupta, Singh, Bhattacharya, Chhokar and Rao.