Browsing by Author "Parmar, Avanish S."

Now showing 1 - 6 of 6
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    Bioengineered dual fluorescent carbon nano dots from Indian long pepper leaves for multifaceted environmental and health utilities
    (Springer Science and Business Media Deutschland GmbH, 2023) Mohapatra, Debadatta; Pratap, Ravi; Pandey, Vivek; Shreya, Singh; Naik, Gaurav Gopal; Mandal, Subhash C.; Otimenyin, Sunday O.; Dubey, Pawan K.; Parmar, Avanish S.; Sahu, Alakh N.
    In this article, we present the synthesis of Piper longum leaves�derived ethanolic carbon dots (PLECDs) using the most simplistic environmentally friendly solvothermal carbonization method. The PLECDs fluoresced pink color with maximum emission at 670�nm at 397�nm excitation. Additionally, the dried PLECDs dissolved in water showed green fluorescence with higher emission at 452�nm at 370�nm excitation. The UV spectra showed peaks in the UV region (271.25�nm and 320.79�nm) and a noticeable tail in the visible region, signifying the efficient synthesis of nano-sized carbon particles and the Mie scattering effect. Various functional groups (�OH, �N�H, �C�H, �C = C, �C�N, and �C�O) were identified using Fourier transform infrared spectroscopy (FTIR). Its nanocrystalline property was revealed by the sharp peaks in the X-ray diffraction (XRD). High-resolution transmission electron microscopy (HRTEM) photomicrograph displayed a roughly spherical structure with a mean size of 2.835�nm. The energy dispersive X-ray (EDAX) and X-ray photoelectron spectroscopy (XPS) revealed the elemental abundance of C, O, and N. The high-performance thin-layer chromatography (HPTLC) fingerprint of PLECDs showed an altered pattern than its precursor (Piper longum leaves ethanolic extract or PLLEE). The PLECDs sensed Cu2+ selectively with a limit of detection (LOD) and limit of quantification (LOQ) of 0.063�?M and 0.193�?M, respectively. It showed excellent cytotoxicity toward MDA-MB-231 (human breast cancer), SiHa (human cervical carcinoma), and B16F10 (murine melanoma) cell lines with excellent in vitro bioimaging outcomes. It also has free radical scavenging activity. The PLECDs also showed outstanding bacterial biocompatibility, pH-dependent fluorescence stability, photostability, physicochemical stability, and thermal stability. � 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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    Carbon dots from an immunomodulatory plant for cancer cell imaging, free radical scavenging and metal sensing applications
    (Future Medicine Ltd., 2021) Mohapatra, Debadatta; Alam, Md. Bayazeed; Pandey, Vivek; Pratap, Ravi; Dubey, Pawan K.; Parmar, Avanish S.; Sahu, Alakh N.
    Aim: This work aimed to develop Tinospora cordifolia stem-derived carbon dots (TCSCD) for cancer cell imaging, free radical scavenging and metal sensing applications. Method: The TCSCDs were synthesized by a simple, one-step, and ecofriendly hydrothermal carbonization method and characterized for their optical properties, morphology, hydrodynamic size, surface functionality, crystallinity, stability, bacterial biocompatibility, in vitro cellular imaging, free radical scavenging and metal sensing ability. Results: The TCSCDs exhibited excellent biocompatibility with dose-dependent bioimaging results in melanoma (B16F10) and cervical cancer (SiHa) cell lines. They exerted good free radical scavenging, Fe3+ sensing, bacterial biocompatibility, photostability, colloidal dispersion stability and thermal stability. Conclusion: The results reflect the potential of TCSCDs for biomedical and pharmaceutical applications. � 2021
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    Facile Synthesis of Graphitic Fungal Carbon Dots for Sensing of Food Adulterants and �Bio-Imaging� of Human Kidney Cell Line
    (John Wiley and Sons Inc, 2023) Kumari, Puja; Pratap, Ravi; Gautam, Veer S.; Das, Megha; Hassan, Nurul; Lahiri, Jayeeta; Mishra, Archana; Yadav, Sanjeev K.; Kharwar, Ravindra N.; Parmar, Avanish S.
    In the current study, a simple and environmentally friendly hydrothermal treatment of fungal biomass for the preparation of carbon nanodots (CND) has been reported. The optical and structural characteristics of the CND have been thoroughly investigated using X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), UV-Visible, and fluorescence spectroscopy methods. Elemental analyses and Fourier Transform Infrared spectroscopy (FTIR) have been used to highlight the surface functionality and composition of the CND.CND have been employed as a fluorescence probe for sensitive and selective detection of rhodamine B (Rh?B) and malachite green (MG) because the presence of food adulterants quenches the fluorescence of the CND. Finally, human embryonic kidney 293 (HEK 293) cell lines have been used to assess the biocompatibility and bio-imaging features of CND. Fluorescence microscopy has been used for qualitative examination of the cellular uptake of CND, while the MTT test has been used for quantitative analysis of the cell-type-dependent uptake of CND. The article further investigates the sensing of food adulterants. Therefore, we have validated the practicality of CND obtained from the new carbon source for versatile applications. � 2023 Wiley-VCH GmbH.
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    In Vitro Cancer Cell Imaging, Free Radical Scavenging, and Fe3+ Sensing Activity of Green Synthesized Carbon Dots from Leaves of Piper longum
    (Springer, 2023) Mohapatra, Debadatta; Pratap, Ravi; Pandey, Vivek; Shreya, Singh; Senapati, Prakash Ch.; Dubey, Pawan K.; Parmar, Avanish S.; Sahu, Alakh N.
    The development of carbon dots via a green synthesis approach from natural products is one of the most researched areas nowadays. Herein, we present the synthesis of Piper longum leaves-derived aqueous carbon dots (PLACDs) via the simplest ecofriendly hydrothermal carbonization method. The PLACDs exhibited excitation-dependent emission behavior with maximum emission at 450�nm at an excitation wavelength of 365�nm. The High-Resolution Transmission Electron Microscopy results showed a quasi-spherical shape with an average size of 4.121�nm. The sharp diffractions of X-ray diffraction revealed its nanocrystalline property. The Energy Dispersive X-ray spectra reflected the presence of carbon, nitrogen, and oxygen. The Fourier-Transform Infrared Spectroscopy disclosed the existence of �OH, �C=C, �C=O, and �C�O�C groups. The PLACDs presented excellent biocompatibility against B16F10 (melanoma) and SiHa (cervical carcinoma) cells lines with concentration-dependent in vitro bioimaging results. It also exhibited antiradical activity with the IC50 value of 0.499�mg/mL and 0.051�mg/mL against DPPH and ABTS.+, respectively. It showed Fe3+ sensing with a lower limit of detection of 0.673�?M. Further, the PLACDs displayed excellent bacterial biocompatibility, pH-dependent fluorescence property, fluorescent ink property, photostability, physical, chemical, and thermal stability. � 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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    Investigating optical, structural and morphological properties of polycrystalline CdTe thin-film deposited by RF magnetron sputtering
    (Elsevier B.V., 2021) Trivedi, Harshita; Gaganpreet; Boochani, Arash; Shagya, Naresh; Lahiri, Jayeeta; Ghorannevis, Zohreh; Parmar, Avanish S.
    In this work, optimization of the physical properties of cadmium telluride (CdTe) thin films has been carried out to improve its performance by the influence of RF power. CdTe Nanocrystalline thin films were characterized by using various techniques such as atomic force microscopy (AFM), field emission scanning electron microscopy (FESEM), and X-ray diffraction (XRD). XRD study revealed that CdTe films are polycrystalline and have preferential growth in (1 1 1) direction. FESEM images showed a continuous and dense morphology of CdTe films on glass substrates. The AFM result shows that the surface roughness of films increases with annealing. Optical properties were investigated with the help of the UV�visible absorption spectrum which showed that the bandgap decreases with an increase in Radio-frequency power. Further, density functional theory (DFT) calculations showed that the optimum bandgap i.e. 1.57 eV for CdTe films deposited at 100 W RF power with 2% N2 is in good agreement with the experimental result. This study showed that CdTe films deposited at 100 W RF power with 2% N2 can be used as an excellent absorbing material for the application of optoelectronic devices solar cells. � 2021 The Author(s)
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    Tinospora cordifolia Leaves Derived Carbon dots for Cancer Cell Bioimaging, Free radical Scavenging, and Fe3+ Sensing Applications
    (Springer, 2022) Mohapatra, Debadatta; Pratap, Ravi; Pandey, Vivek; Dubey, Pawan K.; Agrawal, Ashish K.; Parmar, Avanish S.; Sahu, Alakh N.
    Herein, we report the fabrication of Tinospora cordifolia leaves-derived carbon dots (TCLCDs) from aqueous extract of leaves as carbon source via simple, environmentally friendly, hydrothermal carbonization (HTC) technique. The synthesized TCLCDs were characterized for their physicochemical properties and further explored for in-vitro cancer cell bioimaging, radical scavenging, and metal ion sensing. The synthesized TCLCDs showed excitation-dependent emission property with maximum emission at 435 nm under the excitation of 350 nm. The High-Resolution Transmission Electron Microscopy (HRTEM) results revealed a roughly spherical shape with an average diameter of 5.47 nm. The diffused ring pattern of Selected Area Electron Diffraction (SAED) and halo diffraction pattern of X-ray diffraction (XRD) disclosed their amorphous nature. The Energy Dispersive X-ray (EDX) showed the existence of C, N, and O. The Fourier-transform infrared spectroscopy (FTIR) revealed the presence of -OH, -NH, -CN, and -CH groups. The TCLCDs showed excellent cellular biocompatibility with dose-dependent bioimaging results in melanoma (B16F10) and cervical cancer (SiHa) cell lines. Also, they exhibited excellent scavenging of free radicals with an IC50 value of 0.524 mg/mL & selective Fe3+ ion sensing with a detection limit of 0.414 �M. Further, they exerted excellent bacterial biocompatibility, photostability, and thermal stability. The overall results reflected their potential for in-vitro cancer cell bioimaging, free radical scavenging, and selective Fe3+ ion sensing. � 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.