Browsing by Author "Parmar A.S."

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    Biogenic synthesis of dual-emission chlorophyll-rich carbon quantum dots for detection of toxic heavy metal ions - Hg(ii) and As(iii) in water and mouse fibroblast cell line NIH-3T3
    (Royal Society of Chemistry, 2024) Pratap R.; Hassan N.; Yadav M.; Srivastava S.K.; Chaudhary S.; Verma A.K.; Lahiri J.; Parmar A.S.
    Due to the excessive residues and serious adverse effects, it is urgent to develop an efficient method to detect Hg2+ and As3+ in drinking water to protect human health. In this study, a novel fluorometric sensor based on chlorophyll-rich carbon quantum dots (CQDs) for the detection of Hg2+ and As3+ ions was developed. The dual emitting (blue-green and red) CQDs were synthesized by a one-step solvothermal method using plumeria plant leaves as precursors. When Hg2+ ions were added in the presence of CQDs, the red emission (676 nm) of the CQDs was quenched (OFF), while in the presence of As3+ ions, the blue-green emission (485 nm) was enhanced (ON). The detection limits of 0.99 nM for Hg2+ and 12.15 nM for As3+ were validated for the proposed sensor. The biocompatibility, cytotoxicity, and bioimaging of the CQDs were investigated in the mouse fibroblast cell line NIH-3T3. The cellular uptake was also studied under the influence of Hg2+ and As3+ ions. Moreover, the novel chlorophyll-rich CQD-based fluorometric �ON-OFF� dual probe sensor was successfully applied to detect Hg2+ and As3+ in real water systems as well as in the mouse fibroblast cell line. � 2024 The Royal Society of Chemistry.
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    NiO Nanomaterial Functionalized with Organic Gel Molecules for Heterogeneous Asymmetric Catalysis
    (American Chemical Society, 2024) Gupta P.K.; Kumar N.; Naik K.; Parmar A.S.; Ranganath K.V.
    Chiral inorganic nanomaterials have a wide range of applications including sensing, catalysis, and electronics. Low molecular weight chiral organogels are widely used in organic fluids, pharmaceuticals, drug delivery, self-assembled materials, electrolytes, and liquid crystals. In this study, we report the synthesis of chiral organogels that mimic the properties of Bingham pseudoplastic with Herschel-Bulkley consistency. Furthermore, chiral NiO nanoparticles were prepared in the presence of chiral gels. The development of chiral enantiomerically pure solids for heterogeneous asymmetric catalysis is of great importance in the pharmaceutical industry. The synthesized chiral material can effectively catalyze the asymmetric Michael addition of malonates with chalcones in high yields and with excellent enantioselectivity. The potential of our catalyst was verified by recycling the catalyst in the asymmetric reaction for a number of times. � 2024 American Chemical Society.
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    Sensing of p-nitrophenol using highly selective and sensitive Boran, Nitrogen doped quantum dots
    (Elsevier B.V., 2024) Pratap R.; Pandey S.; Vishal V.; Raghuvanshi I.; Kumar S.; Lahiri J.; Parmar A.S.
    p-Nitrophenol (PNP) is a nitroaromatic compound that poses a significant threat to human health and the environment due to its carcinogenic, mutagenic, cytotoxic, and embryotoxic properties at low concentrations. Therefore, the selective and sensitive detection of PNP is crucial for both human health and environmental monitoring. Boron (B) and Nitrogen (N) doped quantum dots (B,NQDs) have been found to be effective as blue-green luminescent materials for this purpose. These B,NQDs were synthesized using a one-step hydrothermal method, resulting in the formation of highly stable quantum dot. The addition of trace amounts of PNP, the luminescence of the B,NQDs was significantly quenched, which was found to be linearly dependent on the PNP concentration in the range of 100pM to 6 ?M. Further analysis of steady-state absorption and emission, along with photoluminescence decay dynamics, revealed the formation of both static and dynamic quenching complexes. Our simple fluorimetry-based sensor demonstrated an impressive limit of detection (LOD) of 9.08 nM, making it highly selective and sensitive for the detection of PNP. Additionally, the B,NQDs exhibited exceptional stability with respect to pH, UV exposure, salinity, and storage conditions. Finally, we successfully demonstrated the detection of PNP in real water systems and pesticides. � 2024 The Authors
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    Tinospora cordifolia Silver Nanoparticles Attenuated the Lipopolysaccharide-induced Testicular Inflammation in Golden Hamster
    (Springer Science and Business Media Deutschland GmbH, 2024) Mishra N.K.; Yadav K.; Mohanty S.R.; Parmar A.S.; Yadav S.K.; Haldar C.
    Purpose: Testicular inflammation is one of the primary causes of male infertility. Plant-rich remedies are known to reduce testicular inflammation, but the exact mechanism is not known. Tinospora cordifolia (T. cordifolia), an Indian ayurvedic medicinal plant, possesses excellent immunomodulatory properties. We therefore studied the anti-inflammatory potential(s) of green synthesized silver nanoparticles (AgNPs) from T. cordifolia (TC-AgNPs) on the testes of golden hamster. Methods: Aqueous extract of T. cordifolia was used to synthesize the silver nanoparticles (TC-AgNPs), validated by ultra-high-performance liquid chromatography-high-resolution accurate mass spectrometry (UHPLC-HRMS). Physical characterization was done using UV�Vis spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared analysis (FTIR) spectroscopy. Biological parameters were measured using enzyme-linked immunosorbent assay (ELISA), western blot analysis, histopathology, photoacoustic, and ultrasound imaging. Results: The UV�Vis surface plasmon resonance (SPR) band at 420�nm confirmed the formation of TC-AgNPs. TEM revealed the semi-spherical morphology of silver nanoparticles with an average diameter of 15�nm. FTIR spectroscopy revealed the presence of multiple functional groups, indicating the bioformulation of silver nanoparticles with T. cordifolia extract. Biological studies with lipopolysaccharide (LPS) challenged male golden hamsters and demonstrated that TC-AgNPs treatment reduced the serum pro-inflammatory cytokine levels (IL-1?, IL-6, and TNF ?). The testicular photoacoustic and ultrasound imaging indicated a significant improvement in testicular volume and oxygenation level. Western blot analysis suggests that TC-AgNPs reduced inflammation by downregulating NF-?B protein expression. Furthermore, TC-AgNPs alleviated LPS-induced testicular damages along with an increase in serum testosterone level. Conclusion: The synthesized TC-AgNPs have shown strong anti-inflammatory effects against LPS-induced testicular inflammation in male golden hamsters. Lay Summary: Medicinal plants are increasingly used to treat diseases, with inflammation being a common cause of male infertility. Phytoconstituents are safer and more effective anti-inflammatory agents than synthetic ones. Developing plant-based nanoformulations with high therapeutic efficacy and fewer side effects is essential. T. cordifolia is known for its strong immunomodulatory effects and was used to synthesize TC-AgNPs. Our study found that TC-AgNPs reduced pro-inflammatory cytokines and protected against LPS-induced testicular inflammation in golden hamsters. Further, TC-AgNPs improved testicular volume and oxygen saturation, demonstrating potent anti-inflammatory effects. Future Work/Prospective: Future research should focus on how individual bioactive compounds lower inflammation and explain the molecular mechanisms behind it. � The Author(s), under exclusive licence to The Regenerative Engineering Society 2024.