Browsing by Author "Raghuvanshi T.S."

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    Assessment of Trachyspermum ammi essential oil against Aspergillus flavus, aflatoxin B1 contamination, and post-harvest quality of Sorghum bicolor
    (Elsevier Ltd, 2024) Singh P.P.; Jaiswal A.K.; Singh R.; Kumar A.; Gupta V.; Raghuvanshi T.S.; Sharma A.; Prakash B.
    The present investigation explored the antifungal effectiveness of Trachyspermum ammi essential oil (TAEO) against Aspergillus flavus, aflatoxin B1 (AFB1) contamination, and its mechanism of action using biochemical and computational approaches. The GC�MS result revealed the chemical diversity of TAEO with the highest percentage of ?-terpinene (39 %). The TAEO exhibited minimum inhibitory concentration against A. flavus growth (0.5 �L/mL) and AFB1 (0.4 �L/mL) with radical scavenging activity (IC50 = 2.13 �L/mL). The mechanism of action of TAEO was associated with the alteration in plasma membrane functioning, antioxidative defense, and carbon source catabolism. The molecular dynamic result shows the multi-regime binding of ?-terpinene with the target proteins (Nor1, Omt1, and Vbs) of AFB1 biosynthesis. Furthermore, TAEO exhibited remarkable in-situ protection of Sorghum bicolor seed samples against A. flavus and AFB1 contamination and protected the nutritional deterioration. Hence, the study recommends TAEO as a natural antifungal agent for food protection against A. flavus mediated biodeterioration. � 2024 Elsevier Ltd
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    Essential oils as green promising alternatives to chemical preservatives for agri-food products: New insight into molecular mechanism, toxicity assessment, and safety profile
    (Elsevier Ltd, 2024) Prakash B.; Singh P.P.; Gupta V.; Raghuvanshi T.S.
    Microbial food spoilage caused by food-borne bacteria, molds, and associated toxic chemicals significantly alters the nutritional quality of food products and makes them unpalatable to the consumer. In view of potential adverse effects (resistance development, residual toxicity, and negative effects on consumer health) of some of the currently used preservative agents and consumer preferences towards safe, minimally processed, and chemical-free products, food industries are looking for natural alternatives to the chemical preservatives. In this context, essential oils (EOs) showed broad-range antimicrobial effectiveness, low toxicity, and diverse mechanisms of action, and could be considered promising natural plant-based antimicrobials. The existing technical barriers related to the screening of plants, extraction methods, characterization, dose optimization, and unpredicted mechanism of toxicity in the food system, could be overcome using recent scientific and technological advancements, especially bioinformatics, nanotechnology, and mathematical approaches. The review focused on the potential antimicrobial efficacy of EOs against food-borne microbes and the role of recent scientific technology and social networking platform in addressing the major obstacles with EOs-based antimicrobial agents. In addition, a detailed mechanistic understanding of the antimicrobial efficacy of EOs, safety profile, and risk assessment using bioinformatics approaches are summarized to explore their potential application as food preservatives. � 2023 Elsevier Ltd
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    Investigating the Efficacy of Chitosan-Enriched Cuminum cyminum Essential Oil Against Food-Borne Molds, Aflatoxin B1, and Post-Harvest Quality of Arachis hypogaea L.
    (Springer, 2024) Kumar A.; Raghuvanshi T.S.; Gupta V.; Vivekanand; Kohar N.; Prakash B.
    Nanoencapsulation of essential oils exhibited promising applications in food industries, especially in controlling spoilage due to food-borne microbes. In this study, the enhanced antimicrobial efficacy of nanoencapsulated Cuminum cyminum essential oil (Ne-CEO) against food-borne molds, and aflatoxin B1 contamination was observed in a dose-dependent manner. The GC-MS results represent 14 different volatile organic compounds of (CEO) (94.49%), where cuminaldehyde was found to be the major one. The interaction of the Cuminum cyminum essential oil (CEO) and chitosan nanoparticles (CSNPs) was confirmed with the Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. The Ne-CEO exhibited superior antimicrobial effects compared to non-encapsulated CEO and inhibited the growth of selected mold species (0.3�0.5 �L/mL) and aflatoxin B1 (AFB1) secretion at 0.4 �L/mL. The probable toxicity mechanism results show membrane impairment and cellular homeostasis linked with decreased ergosterol content, increased cation leakage, impairment in antioxidant defenses, carbon metabolism, and transcriptional genes (Ver-1 and Nor-1) functioning of AFB1 biosynthesis. Furthermore, during the six months in-situ trial, Ne-CEO (0.4 �L/mL) remarkably protected the biodeterioration of A. hypogaea seed samples against A. flavus growth and AFB1 contamination, thus enhancing its practical application as a plant-based food preservative to enhance the shelf-life of food commodities. � The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
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    Nanofabrication of citronellal with chitosan biopolymer to boost its efficacy against aflatoxin B1 and Aspergillus flavus mediated biodeterioration of active ingredient of Piper longum
    (Elsevier Ltd, 2024) Kumar A.; Raghuvanshi T.S.; Pratap S.; Kumar H.; Prakash B.
    The study reports the efficacy of nanofabricated citronellal inside the chitosan biopolymer (Ne[sbnd]Cn) against Aspergillus flavus growth, aflatoxin B1 (AFB1) production, and active ingredient biodeterioration (Piperine) in Piper longum L. The prepared Ne[sbnd]Cn was characterized by Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), and Fourier Transform Infrared Spectroscopy (FTIR). The results revealed that the Ne[sbnd]Cn exhibited distantly improved antifungal (1.25 ?L/mL) and AFB1 inhibition (1.0 ?L/mL) compared to free Cn. The perturbances in membrane function, mitochondrial membrane potential, antioxidant defense system, and regulatory genes (Ver-1 and Nor-1) of AFB1 biosynthesis were reported as probable modes of action of Ne[sbnd]Cn. The Ne[sbnd]Cn (1.25 ?L/mL) effectively protects the P. longum from A. flavus (78.8%), AFB1 contamination (100%), and deterioration of Piperine (62.39%), thus demonstrating its potential as a promising novel antifungal agent for food preservation. � 2024 Elsevier Ltd