Browsing by Author "Ravikanth G."

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    In-vitro Anti-inflammatory Potential of Standardized Rottlerin Enriched Fraction of Mallotus philippensis Muell. Arg Anti-inflammatory Potential of Rottlerin Enriched Fraction of Mallotus philippensis
    (Springer, 2024) Tripathi N.; Mandrah K.; Goel B.; Bhardwaj N.; Paswan V.K.; Ravikanth G.; Roy S.K.; Jain S.K.
    A rottlerin enriched fraction was prepared from extract of Mallotus philippensis Muell. Arg leaves in ethyl acetate using HP-20 dianion polymeric resin. The procedure of extraction and fractionation was optimized to get fractions enriched with rottlerin. The different fractions obtained were then evaluated for in-vitro anti-inflammatory activity for COX inhibition. The most active fraction (fraction 6) was subjected to standardization by HPLC and found to contain 70% rottlerin, that could be responsible for the highest activity of the fraction. Further, the chromatographic purification of the active fraction yielded rottlerin (1), isoallorottlerin (2), 8-cinnamoyl-5,7-dihydroxy-2,2,6-trimethyl- 3-chromene (3), and mallotophilippen F (4) as markers. The rottlerin-enriched fraction possessed good in-vitro anti-inflammatory activity against both COX-1 and COX-2 enzymes and was more active than the reference standards drugs viz. etoricoxib and ibuprofen. The optimized protocol for extraction and resin-based fractionation was found to be efficient, reproducible, time-saving, and eco-friendly as operated in semi-aqueous conditions. � The Author(s), under exclusive licence to The National Academy of Sciences, India 2024.
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    Isolation of cytotoxic cycloartane triterpenoids from Dysoxylum malabaricum
    (Royal Society of Chemistry, 2024) Bhardwaj N.; Sharma V.K.; Tripathi N.; Pimpre K.; Sonti R.; Ravikanth G.; Koch B.; Jain S.K.
    New cycloartane triterpenoids (2-4) have been recently discovered in the bark of Dysoxylum malabaricum. The extraction process involved the utilization of a cytotoxic dichloromethane-methanol extract, which underwent meticulous bioassay-guided fractionation employing high-performance liquid chromatography. A strategic LCMS-DNP-based dereplication approach was used to target the new compounds. Subsequently, the isolated compounds were thoroughly characterized utilizing nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectrometry (HRESIMS) data, and their absolute configurations were ascertained through electronic circular dichroism (ECD) calculations. In the context of biological activity, the compounds were evaluated for their cytotoxic potential against a panel of breast cancer cell lines and human normal embryonic kidney cancer cells. 2 demonstrated remarkable cytotoxicity, exhibiting an IC50 value of 18 ?M against the T-47D breast cancer cell line, concurrently eliciting apoptotic cell death. The findings of this study emphasize the substantial potential of 2 as a promising candidate for further development as an anti-cancer agent. � 2024 The Royal Society of Chemistry.
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    New ring-A modified cycloartane triterpenoids from Dysoxylum malabaricum bark: Isolation, structure elucidation and their cytotoxicity
    (Elsevier Inc., 2024) Bhardwaj N.; Gupta P.; Tripathi N.; Chakrabarty S.; Verma A.; Kumari S.; Gautam V.; Ravikanth G.; Jain S.K.
    The Genus Dysoxylum (Meliaceae) consists of approximately 80 species that are abundant in structurally diverse triterpenoids. The present study focused on isolating new triterpenoids from the bark of Dysoxylum malabaricum, one of the predominant species of Dysoxylum present in India. The methanol-dichloromethane bark extract was subjected to LCMS profiling followed by silica gel column chromatography and HPLC analysis to target new compounds. Two new ring A-modified cycloartane-type triterpenoids (1 and 2) were isolated from the bark extract. Spectroscopic methods like NMR, HRESIMS data, and electronic circular dichroism calculations elucidated the structures and absolute configurations of the isolated compounds. These compounds were evaluated for their cytotoxic potential against breast cancer cells and displayed notable cytotoxicity. Compound 1 exhibited the highest cytotoxicity against the MDA-MB-231 cells and induced apoptotic cell death. Also, it was able to inhibit glucose uptake and increase nitric oxide production in breast cancer cells. � 2024 Elsevier Inc.