BHU Institutional Repository :: Browsing by Author "Saha, Pajeb"

Browsing by Author "Saha, Pajeb"

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Genome-wide identification of bZIP transcription factor family in Artemisia annua, its transcriptional profiling and regulatory role in phenylpropanoid metabolism under different light conditions
(Springer, 2023) Rai, Nidhi; Kumari, Sabitri; Singh, Sneha; Saha, Pajeb; Pandey-Rai, Shashi
The basic leucine zipper (bZIP) protein transcription factors are known to modulate development, plant growth, metabolic response, and resistance to several biotic and abiotic stressors and have been widely studied in the model plant Arabidopsis thaliana. However, no comprehensive information about the bZIP transcription factor family in Artemisia annua has been explored to date. In this genome-wide study, we identified 61 bZIP TFs after removing false positives and incomplete sequences from Artemisia annua. Seven highly expressed homolog AabZIP TF genes under UV-B and differential light conditions in different tissues were identified from the publicly available microarray dataset as having their cis-regulatory elements involved in, flavonoids biosynthesis, seed-specific gene regulation, stress responses, and metabolic regulation. In-silico analysis and electrophoretic mobility shift assay (EMSA) confirmed the interaction of AabZIP19 TF over the AaPAL1 promoter in order to regulate the phenolics and flavonoid biosynthesis via the phenylpropanoid pathway. Further, RT-PCR analysis has been carried out to validate the transcript levels of selected AabZIP genes under white light, red light, blue light (45�min), and UV-B exposure (12 and 24�h). These genes have their highest expression levels under UV-B and blue light exposure, in contrast with white light. Therefore, the detection of ROS through staining confirms the accumulation of superoxide radicals and H2O2, and in addition to reducing ROS accumulation under UV-B and blue light irradiation, total phenols and flavonoids are significantly enhanced. This study laid the groundwork for deciphering the possible role of AabZIP TFs under different light stress-responsive conditions and in the regulation of secondary metabolism. � 2023, Prof. H.S. Srivastava Foundation for Science and Society.
Pharmacology of Natural and Synthetic Phytoprotectants: Application and Consequences in Cancer Therapies
(Springer Nature, 2023) Singh, Sneha; Saha, Pajeb; Rai, Nidhi; Kumari, Sabitri; Pandey-Rai, Shashi
Cancer represents one of the most fatal health issues, claiming the lives of millions of people each year. Tumorous growths can develop in almost any portion of the body and migrate to different parts. There are numerous treatment approaches available for cancer such as radiation therapy, photodynamic therapy, and cancer vaccinations. However, in most cases, they have adverse side effects. Thus, anticancer medications with higher efficiency and fewer side effects are desperately needed. Plants are a prospective source of such compounds. Natural plant bioactive substances have been used in traditional medicine since the dawn of humanity. These metabolites have also been implicated in providing protection to plants under various environmental influences, such as the influence of UV-B. Plant-based natural secondary metabolites/phytochemicals and their derivatives have great potential in the suppression of cancer development and metastasis. These biologically active compounds can be isolated from various plant parts, such as leaves, stems, barks, flowers, rhizomes, roots, and seeds. The natural bioactive compounds produced by plants during secondary metabolism have great pharmacological importance, especially as anticancer agents. Therefore, this chapter is an attempt to summarize the importance of various plant-derived compounds and their mechanism of action, which can be used in cancer therapies as anticancer agents. � The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2023.
PLANT CIRCADIAN RHYTHM: A BIOLOGICAL CLOCK AS DEVELOPMENTAL AND METABOLIC REGULATOR
(Nova Science Publishers, Inc., 2022) Rai, Nidhi; Kumari, Sabitri; Saha, Pajeb; Apoorva; Rai, Sanjay Kumar; Meena, Ram Prasad; Pandey-Rai, Shashi
Plants have an internal biological system that receives differential environmental fluctuations/stimuli such as temperature and light controlling circadian rhythm for maintenance of growth and developmental processes. These biological rhythms are regulated by the interaction of certain external signals and internal receptors. In plants, it is complex networking within transcription factors that functions in feedback loops. These light-induced phototropic controls are mediated by photoreceptors like phytochromes, cryptochrome, phototropin and master genes/regulators for floral development. These responses are genetic in nature and have master clock genes which further regulates many copies of the master transcription factor that are responsible for regulating/switch-on many important genes of metabolism by binding with the promoter region of target genes. The diurnal behavior in plants has been observed because of the existence of a feedback loop and a phosphorylation-dephosphorylation cycle. The light and temperatures positively regulate the induction of various genes along with a set of polycomb gene. Many long non-coding RNAs, micro-RNA and RNAdependent polymerases are indispensable parts of the diurnal cycle in plants. Environmental signals are involved in activating clock genes, and clock repressor circuits work to alienate and degrade these extrinsic gene activation pathways. Most transcription factors are cyclic and these subclasses can regulate clock parameters. Transcriptional regulators and associated chromatids that control transcriptional regulation are only one step in a multistep regulatory network. Post-translational relaxation, nuclear-cytoplasmic dissociation, RNA splicing and proteolytic functions participate in the stimulation. Homogenization of all these activities leads to the generation and sustainable facilitation of the robust rhythm and response to the diurnal variations of the environment. The purpose of this chapter is to explain the physiological and molecular mechanisms of the circadian clocks of plants, including biochemistry, and to demonstrate the function/role of the circadian clock in metabolic, physiological processes and plant behavior. � 2022 by Nova Science Publishers, Inc.
Unravelling triterpenoid biosynthesis in plants for applications in bioengineering and large-scale sustainable production
(Elsevier B.V., 2023) Singh, Sneha; Apoorva; Saha, Pajeb; Rai, Nidhi; Kumari, Sabitri; Pandey-Rai, Shashi
Plants are major factories for the biosynthesis of valuable bioactive compounds having pharmaceutical applications. These phytocompounds are synthesised via the diversion of primary metabolites toward secondary metabolic pathways. The most varied class of metabolites are triterpenoids that are biosynthesized through the MVA-MEP pathways, leading to the production of oxidosqualene followed by cyclization, and enzymatic modifications. Triterpenoids play a significant role in growth, development, reproductive behaviour, plant adaptation, and plant-to-plant communication. Various signals, through the signal transduction mechanism, are transmitted through receptors from cell to cell by a series of molecular events to initiate/ activate the cascade of the triterpenoid biosynthesis network. In this review, different naturally occurring classes of triterpenoids with their medicinal potential have been summarized. Further, this review provides insight into the current status of its synthesis through heterologous gene expression, structure elucidation, biosynthetic regulation via transcriptional regulation, and miRNAs. Recently, triterpenoids are in demand due to their variety of pharmacological importance which requires a fast rate of production. Furthermore, to ramp up the synthesis of these vital triterpenoids, the applications of modern bioengineering technologies and green nanoparticle synthesis, have been also highlighted here, offering sustainable alternatives for their large-scale production. � 2023 Elsevier B.V.

Browsing by Author "Saha, Pajeb"

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