Browsing by Author "Sanyal I."

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    Development of a high-frequency in vitro regeneration system in Indian lotus (Nelumbo nucifera Gaertn.)
    (Springer, 2024) Verma R.; Yadav A.; Gupta R.K.; Sanyal I.
    The Indian lotus (Nelumbo nucifera Gaertn.) is a popular ornamental plant and a source of traditional herbal medicine. Its various parts are widely used in the pharmaceutical, cosmeceutical, and nutraceutical industries. The recent study aimed to develop a high-frequency in vitro regeneration system in Indian lotus. This study utilizes the pink lotus cultivar from the Botanical Garden of CSIR-NBRI Lucknow. The study was successfully achieved through direct and indirect methods using different plant growth regulators (PGRs). A direct regeneration system was established using explants shoot apical meristem and plumule cultured on SIM supplemented with 17 combinations and concentrations of BAP and NAA. Both explants produced the highest number of shoots with a combination of 4.44�?M BAP and 0.55�?M NAA. The highest number of shoots per explant 25 � 1.0 was developed from the shoot apical meristem, while the plumule explant developed 16.3 � 0.5 shoots per explant. Thereafter, the plantlets were transferred to LRIM, which contained 17 combinations and concentrations of NAA or IBA and BAP. The maximum number of roots, per explant 23.6 � 0.5, was developed from shoot apical meristem using 2.22�?M NAA and 0.54�?M BAP. The highest number of roots, per explant 23 � 1.0, was developed from the plumule using 4.44�?M IBA. Indirect somatic embryogenesis has been established through callus culture. The leaf segments were cultured onto a callus induction medium supplemented with ten combinations of 2,4-D and BAP. The high-frequency callus formation 24.33 � 0.5 was obtained with a 5.0�?M 2,4-D and 1.0�?M BAP combination. All developmental stages at the proembryo, globular, heart, torpedo, and mature embryos were formed on concentrations of 2,4-D and BAP. After inducing shoot and root growth, well-developed plantlets were transferred to the greenhouse, resulting in a success rate of 18.47%. � The Society for In Vitro Biology 2024.
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    Overexpression of miR166 in Response to Root Rhizobacteria Enhances Drought Adaptive Efficacy by Targeting HD-ZIP III Family Genes in Chickpea
    (Springer Science and Business Media Deutschland GmbH, 2024) Yadav A.; Kumar S.; Verma R.; Narayan S.; Gupta U.; Lata C.; Rai S.P.; Sanyal I.
    Using the transgenic approach, the current study investigated the tripartite interaction of miRNA166, Plant Growth Promoting Rhizobacteria (PGPR), and chickpea crops in response to drought. miR166, an evolutionarily conserved small RNA, was cloned and transformed in a homologous manner. This Car-miR166 is reported in our previous research to have drought-enduring roles in response to microbial candidates. A Pseudomonas putida strain RA (MTCC5279) is used as a PGPR for the whole study. The overexpressed lines generated using tissue-culture practice were functionally validated with physiological parameters studied using Li-Cor 6400XT, including photosynthesis rate, transpiration rate, water-use efficiency, and electron transport rate. We also studied the relative water content of the overexpressed lines in comparison to treated control plants. In biochemical methods, we studied the accumulation of proline, superoxide dismutase, peroxidase, catalase, H2O2 and lipid peroxidation levels. miR166 has its target as ATHB15 (Homeobox-leucine zipper protein-15) validated using 5� RNA Ligase-Mediated Rapid Amplification of cDNA Ends (RLM-RACE) experiment. At the molecular levels, we carried out the stem-loop quantitative real-time (qRT) PCR analysis of miR166 and the expression analysis of ATHB15 in transgenic lines. As per our study, the results reported that the transgenic lines showed a positive interaction of miR166 with PGPR, resulting in drought stress mitigation and better plant survival in harsh drought conditions. In conclusion, the physiology, biochemistry, and molecular expression levels of Car-miR166 (Cicer arietinum L.) in transgenic lines in response to PGPR support enhanced growth and development in response to PGPR in transgenic lines under drought. � The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2024.