Browsing by Author "Sharma, Shilpa"

Now showing 1 - 5 of 5
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    Cellular experiments to study the inhibition of c-Myc/MAX heterodimerization
    (Academic Press Inc., 2022) Singh, Ashutosh; Sharma, Shilpa; Kumar, Praveen; Garg, Neha
    The c-Myc oncogene is a master regulator of cancer cell metabolism, which controls a variety of pathways, including cell proliferation, cell cycle, apoptosis, and epigenetics. Belonging to the bHLH family of transcription factors, c-Myc forms a heterodimeric complex with another bHLH family protein MAX. c-Myc deregulation is reported in most cancers. This heterodimeric complex is a potent transcription factor that controls the expression of the target gene by binding to the E-box sequence and thereby controlling cancer cell proliferation. c-Myc in isolation has a partially folded structure and cannot carry the transcription. However, its heterodimerization provides the ability to bind DNA and carry out the regulatory function. Therefore, heterodimerization of c-Myc and Max is of great interest for cancers, and it has always been considered a target for cancer therapy. This book chapter will present a detailed protocol of cellular experiments employed to validate the in vitro potency of c-Myc inhibitor candidates to search for a novel c-Myc-targeted neoplastic drug. � 2022 Elsevier Inc.
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    Giant bilateral pediatric thymolipoma presenting with respiratory distress
    (Elsevier Inc., 2021) Sharma, Kanika; Sharma, Shilpa; Seth, Rachna; Gupta, Devendra K.
    Thymolipoma is a rare thymic mass usually incidentally diagnosed. We present the case of an 8-year old child who presented with breathlessness and cough. On evaluation, she had a giant anterior mediastinal mass involving bilateral hemithorax (right > left) which was diagnosed as thymolipoma. The child was started on steroids, to which she responded very well initially, but after few months the thymic mass gradually increased again and respiratory distress reccured. Subsequently total excision of mass was done followed by gradually tapering of steroids. The child had excellent recovery and is asymptomatic at 2.5 years follow up. � 2021 The Authors
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    Molybdenum-based hetero-nanocomposites for cancer therapy, diagnosis and biosensing application: Current advancement and future breakthroughs
    (Elsevier B.V., 2021) Dhas, Namdev; Kudarha, Ritu; Garkal, Atul; Ghate, Vivek; Sharma, Shilpa; Panzade, Prabhakar; Khot, Shubham; Chaudhari, Pinal; Singh, Ashutosh; Paryani, Mitali; Lewis, Shaila; Garg, Neha; Singh, Narinder; Bangar, Priyanka; Mehta, Tejal
    In recent years, there have been significant advancements in the nanotechnology for cancer therapy. Even though molybdenum disulphide (MoS2)-based nanocomposites demonstrated extensive applications in biosensing, bioimaging, phototherapy, the review article focusing on MoS2 nanocomposite platform has not been accounted for yet. The review summarizes recent strategies on design and fabrication of MoS2-based nanocomposites and their modulated properties in cancer treatment. The review also discussed several therapeutic strategies (photothermal, photodynamic, immunotherapy, gene therapy and chemotherapy) and their combinations for efficient cancer therapy along with certain case studies. The review also inculcates various diagnostic techniques viz. magnetic resonance imaging, computed tomography, photoacoustic imaging and fluorescence imaging for diagnosis of cancer. � 2020 Elsevier B.V.
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    Stimuli responsive and receptor targeted iron oxide based nanoplatforms for multimodal therapy and imaging of cancer: Conjugation chemistry and alternative therapeutic strategies
    (Elsevier B.V., 2021) Dhas, Namdev; Kudarha, Ritu; Pandey, Abhijeet; Nikam, Ajinkya N.; Sharma, Shilpa; Singh, Ashutosh; Garkal, Atul; Hariharan, Kartik; Singh, Amanpreet; Bangar, Priyanka; Yadhav, Dattatray; Parikh, Dhaivat; Sawant, Krutika; Mutalik, Srinivas; Garg, Neha; Mehta, Tejal
    Cancer being one of the most precarious and second most fatal diseases evokes opportunities for multimodal delivery platforms which will act synergistically for efficient cancer treatment. Multifunctional iron oxide magnetic nanoparticles (IONPs) are being studied for few decades and still attracting increasing attention for several biomedical applications owing to their multifunctional design and intrinsic magnetic properties that provide a multimodal theranostic platform for cancer therapy, monitoring and diagnosis. The review article aims to provide brief information on various surface chemistries involved in modulating IONPs properties to exhibit potential therapy in cancer treatment. The review addresses structural, magnetic, thermal and optical properties of IONPs which aids in the fabrication of efficient multimodal nanoplatform in cancer therapy. The review discussed the pharmacokinetics of IONPs and attributes influencing them. This review inculcates recent advancements in therapies, focused on tumor-microenvironment-responsive and targeted therapy along with their eminent role in cancer diagnosis. The concept of stimuli-responsive including endogenous, exogenous and dual/multi stimuli-based delivery platform demonstrated significantly enhanced anticancer therapy. Several therapeutic approaches viz. chemotherapy, radiotherapy, immunotherapy, hyperthermia, gene therapy, sonodynamic therapy, photothermal, photodynamic-based therapy along with biosensing and several toxicity aspects of IONPs have been addressed in this review for effective cancer treatment. � 2021
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    Varied parameters and utility of the anal position index: a systematic review and meta-analysis
    (Springer Science and Business Media Deutschland GmbH, 2021) Sharma, Shilpa; Perumal, Vanamail; Sharma, Kanika; Gupta, Devendra K.
    Purpose: The anal position index (API) was described in 1984 by Reisner et al. Since then, it has been measured in different ethnic populations and its utility in various medical conditions have been explored. We aimed to review the literature regarding the various values reported analytically. Method: A Pubmed Search was carried out with the terms Anal Position Index. There were 158 articles hit by the search. The description of the API was uniform in all studies, described as ratio of anus�fourchette distance in girls and anus�scrotum distance in boys to the distance between coccyx and fourchette/scrotum. 18 relevant studies were included describing the values in different ethnic groups and describing its utility. One study was excluded from statistics due to different landmark for measurement. The studies were grouped into 4. Group A:B:C:D comprised of Newborns:Infants:All age groups:Constipated children. We carried out meta-analysis to estimate effect size (mean difference) using STATA software version 16.0. Results: The different ethnic populations in which API were measured, and were Indian (2), Turkish (3), Israel (2), Taiwanese, Thai, Iranian, Spanish, Italian, and Mexican. The API was higher by 0.129 in boys. The mean API in males was 0.53:0.54:0.48:0.52 in Group A:B:C:D with an overall mean (SD) API of 0.51 (0.04). The mean API in females was 0.40:0.40:0.38:0.37 in Group A:B:C:D with an overall mean (SD) API of 0.40(0.03). Two studies were done in mice and showed the relation of API to intrauterine exposure to androgens. In children, API was mostly used to diagnose an anterior ectopic anus and see correlation with constipation. The abnormal values varied from less than 0.30�0.34 in girls and less than 0.41�0.46 in boys. The effect size by regions showed that the estimated effect size for all the regions were within 95% Confidence limits of overall estimate (0.13: 95% CI: 0.13�0.14). Therefore, it can be inferred that there was no significant ethnic variation in the study parameter. The anal position in relation to genitalia was measured in nulliparous women, menopause women, and women with levator deficiency. It was affected in vulvovaginal atrophy. Dichlorodiphenyldichloroethylene exposure during the first trimester of pregnancy was reported to alter the anal position in male infants. Conclusion: The anteriorly positioned anus has been associated with constipation. API been measured in various ethnic�populations with insignificant variations. The API is�significantly (p < 0.05) higher in males. The presence of constipation does not seem to alter API. One should adopt a single method for measurement. API should not be considered at the sole indication for any surgical intervention. � 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.