Browsing by Author "Singh, Anil K."
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Publication An umbrella review on ethnomedicinal, pharmacological and phytochemical profile of pointed gourd (Trichosanthes dioica Roxb.): A bioactive healthy vegetable of Indian subcontinent(Elsevier Ltd, 2023) Sharma, Swati; Sinha, Ritika; Singh, Anil K.; Meena, Yogendra; Pongener, Alemwati; Sharma, Rohit; Behera, Tusar Kanti; Barman, KalyanTrichosanthes dioica Roxb. is a widely cultivated cucurbitaceous vegetable of tropical and sub-tropical regions. Its unripe fruits are consumed as vegetable. This review aims to present an umbrella overview of botany, ethnomedicinal uses, nutritional and phytochemical profile of T. dioica and compilation of research confirming many pharmacological and healing properties of its plant parts. Importantly, several reports from India and Bangladesh have confirmed its ethnomedicinal usage in many biomedical conditions. Bioactive compounds like triterpenes, polyphenols present in different parts of T. dioica make it a powerhouse of health, immunity and nutrition. Different parts of T. dioica including root, stem, leaf, fruit and seed exhibit wide range of therapeutic properties including antidiabetic, anti-tumour, antiulcer, antioxidant, anti-inflammatory, antidiarrheal, antihyperlipidemic, antipyretic, wound healing, laxative, antinociceptive and hepato-protective. Results signified that it may be useful to re-look to find possibilities for using its active metabolites in contemporary medicine and nutraceuticals. � 2023Publication Effect of foliar treatment of putrescine on growth and flowering of annual gypsophila (Gypsophila elegance)(Indian Council of Agricultural Research, 2023) Sahu, Tijeshwari; Tsomu, Thupten; Singh, Anil K.; Barman, Kalyan; Sisodia, Anjana; Kumar, Yogendra; Kumari, Priyanka; Tasung, AmpeeIn this research work, we attempted to know the positive response of putrescine on plant growth and flowering of annual flower gypsophila. Putrescine is important because it has been shown that exogenous treatment can enhance the production or accumulation of bioactive chemicals that show a positive impact on plant growth and development. Significant results were obtained in various growth and flowering parameters of annual gypsophila due to foliar treatment with putrescine. Maximum plant height, maximum plant spread, maximum number of leaves perplant, maximum number of primary branches and secondary branches per plant at 60 DAT and 90 DAT as well as maximum number of flower clusters per branch and maximum number of flower clusters per plant was recorded from 1.50 mmol of putrescine application. � 2023 Indian Council of Agricultural Research. All rights reserved.Publication EFFECT OF PINCHING AND GROWTH PROMOTING CHEMICALS ON GROWTH AND SEED ATTRIBUTES IN VERBENA (Verbena hybrida)(Punjab Agricultural University, 2023) Bhusal, Usha; Singh, Anil K.; Sisodia, Anjana; Barman, Kalyan; Padhi, Minakshi; Kushwaha, AkashThe purpose of this study was to investigate the impact of pinching and growth promoting chemicals on the growth and seed attributes in verbena flower crop. Specifically, the study aimed to identify the effect of GA3 and salicylic acid at varying concentrations on plant growth and seed yield as well as determine the impact of pinching on these factors. Hence, an experiment was conducted to find out the effect of pinching and growth promoting chemicals on the growth and seed attributes of verbena. Growth promoting chemicals used in the experiment were GA3 and Salicylic Acid (SA) @ 100, 150 and 200 ppm each. The chemicals were foliar sprayed at 21 days after transplanting followed by pinching at 40 days after transplanting. Healthy seedlings were transplanted in plots of size 6 � 3 m with spacing of 60 � 50 cm. The trial was conducted using Randomized Block Design with three replications. in each treatment. The findings suggest that pinching at 40 days after transplanting can significantly improve the growth and seed attributes, where, pinching resulted in significantly greater plant spread, secondary branches, dry weight of leaves, leaf are index, seed yield/shoots and seed yield/plant. Meanwhile, GA3 at 200 ppm produced the highest test weight. The use of SA at 200 ppm also showed promising results in terms of growth and leaf area index in verbena. Present study highlights the positive impact of pinching and growth promoting chemicals at varying concentrations. It also suggests that incorporating pinching and growth promoting chemicals in cultivation of verbena can significantly improve the crop yield and quality. � 2023, Punjab Agricultural University. All rights reserved.Publication Exogenous polyamine treatment preserves postharvest quality, antioxidant compounds and reduces lipid peroxidation in black plum fruit(Elsevier B.V., 2022) Mishra, Shatakashi; Barman, Kalyan; Singh, Anil K.; Kole, BasudevBlack plum (Syzygium cumini L. Skeels) is a tropical and sub-tropical fruit having several medicinal properties, especially in curing diabetes. Due to non-climacteric nature, the fruit is harvested at ripe stage. However, presence of thin skin and rapid softening make the fruit highly susceptible to moisture loss and microbial attack, which drastically reduce its shelf life. Black plum, due to its high perishability cannot be stored more than 2 � 3 days at ambient conditions. In the present study, the effect of pre-storage polyamines treatment on quality and storability of black plum fruit was investigated. Ripe fruits were treated with putrescine (PUT) and spermine (SPM) each at 0.5 mM and 1.0 mM concentration by immersion method while, control fruit were treated with distilled water. The fruit were stored at ambient conditions (27 � 3 �C, 85 � 5% RH) for 6 days. Results revealed that 1.0 mM PUT treatment was most effective in minimizing weight loss and spoilage of about 1.5-fold compared to control. The treated fruits also retained higher anthocyanins and minimized lipid peroxidation than control. The loss of bioactive compounds like total phenolics, flavonoids, ascorbic acid and antioxidant capacity also minimized in response to polyamine treatments. The soluble solids content retained higher in 1.0 mM PUT-treated fruits but, polyamine treatments did not affect acid content. The findings of the study can be beneficially exploited in extending storability, long distant marketing and reducing postharvest loss of black plum fruit. � 2021 SAABPublication Exogenous Putrescine Treatment Maintains Postharvest Quality and Delays Senescence of Guava Fruit(Springer Science and Business Media Deutschland GmbH, 2023) Thapa, Sandhya; Barman, Kalyan; Singh, Anil K.Guava is an important subtropical fruit, valued for its characteristic flavour and high vitamin�C content. However, the fruit ripens rapidly after harvest, making it soft, mealy, and prone to spoilage, thus reducing its marketability within a�short period. This study aimed to investigate the response of postharvest putrescine (PUT) treatment on quality and senescence of guava. Fruits were treated with PUT (0.5?mM, 1.0?mM, or 1.5?mM) and stored at ambient conditions (27?� 3?�C). Among the treatments, 1.0?mM PUT was the most effective in minimizing weight loss and decay loss over control, by ~�20.6% and 28%, respectively. Ripening and senescence were delayed by PUT treatments, as indicated by high chlorophyll retention and minimal lipid peroxidation and carotenoid content. Ascorbic acid, phenolics, flavonoids, antioxidant capacity, and radical scavenging activity were best maintained with the 1.0?mM PUT treatment. However, total soluble solids and acidity content of fruit were less affected by this treatment. � 2022, The Author(s), under exclusive licence to Der/die Autor(en), exklusiv lizenziert an Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature.Publication Fish hook technique for nucleus management in manual small-incision cataract surgery: An Overview(Wolters Kluwer Medknow Publications, 2022) Anand, Abhishek; Singh, Vivek; Kumari, Rashmi; Mishra, Deepak; Kumar, Rajesh; Singh, Anil K.Manual small-incision cataract surgery (MSICS) preserves its utility as a cost-effective and efficient strategy for mitigating cataract-associated blindness. Numerous techniques of nucleus delivery in MSICS have been described in the literature. The fish hook technique of nucleus extraction was advocated in 1997 and has been popular for high-volume surgery in limited pockets of the world. This article describes in detail the steps involved in the construction of a fish hook, tips of nucleus extraction with the help of a fish hook, and the pros and cons of fish hook-based MSICS through text, diagram, and video supplement. � 2022 Indian Journal of Ophthalmology.Publication Multi-location evaluation of mungbean (Vigna radiata L.) in Indian climates: Ecophenological dynamics, yield relation, and characterization of locations(Frontiers Media S.A., 2022) Parihar, Ashok K.; Gupta, Sanjeev; Hazra, Kali K.; Lamichaney, Amrit; Sen Gupta, Debjyoti; Singh, Deepak; Kumar, Raju; Singh, Anil K.; Vaishnavi, Rakesh; Jaberson, M. Samuel; Das, Sankar P.; Dev, Jai; Yadav, Rajesh K.; Jamwal, B.S.; Choudhary, B.R.; Khedar, O.P.; Prakash, Vijay; Dikshit, Harsh K.; Panwar, R.K.; Katiyar, Manoj; Kumar, Pankaj; Mahto, C.S.; Borah, H.K.; Singh, M.N.; Das, Arpita; Patil, A.N.; Nanda, H.C.; Kumar, Vinod; Rajput, Sumer D.; Chauhan, D.A.; Patel, M.H.; Kanwar, Raja R.; Kumar, Jitendra; Mishra, S.P.; Kumar, Hitesh; Swarup, Indu; Mogali, Suma; Kumaresan, D.; Manivannan, Narayana; Gowda, M. Byre; Pandiyan, Muthaiyan; Rao, Polneni J.; Shivani, D.; Prusti, A.M.; Mahadevu, P.; Iyanar, K.; Das, SujataCrop yield varies considerably within agroecology depending on the genetic potential of crop cultivars and various edaphic and climatic variables. Understanding site-specific changes in crop yield and genotype � environment interaction are crucial and needs exceptional consideration in strategic breeding programs. Further, genotypic response to diverse agro-ecologies offers identification of strategic locations for evaluating traits of interest to strengthen and accelerate the national variety release program. In this study, multi-location field trial data have been used to investigate the impact of environmental conditions on crop phenological dynamics and their influence on the yield of mungbean in different agroecological regions of the Indian subcontinent. The present attempt is also intended to identify the strategic location(s) favoring higher yield and distinctiveness within mungbean genotypes. In the field trial, a total of 34 different mungbean genotypes were grown in 39 locations covering the north hill zone (n = 4), northeastern plain zone (n = 6), northwestern plain zone (n = 7), central zone (n = 11) and south zone (n = 11). The results revealed that the effect of the environment was prominent on both the phenological dynamics and productivity of the mungbean. Noticeable variations (expressed as coefficient of variation) were observed for the parameters of days to 50% flowering (13%), days to maturity (12%), reproductive period (21%), grain yield (33%), and 1000-grain weight (14%) across the environments. The genotype, environment, and genotype � environment accounted for 3.0, 54.2, and 29.7% of the total variation in mungbean yield, respectively (p < 0.001), suggesting an oversized significance of site-specific responses of the genotypes. Results demonstrated that a lower ambient temperature extended both flowering time and the crop period. Linear mixed model results revealed that the changes in phenological events (days to 50 % flowering, days to maturity, and reproductive period) with response to contrasting environments had no direct influence on crop yields (p > 0.05) for all the genotypes except PM 14-11. Results revealed that the south zone environment initiated early flowering and an extended reproductive period, thus sustaining yield with good seed size. While in low rainfall areas viz., Sriganganagar, New Delhi, Durgapura, and Sagar, the yield was comparatively low irrespective of genotypes. Correlation results and PCA indicated that rainfall during the crop season and relative humidity significantly and positively influenced grain yield. Hence, the present study suggests that the yield potential of mungbean is independent of crop phenological dynamics; rather, climatic variables like rainfall and relative humidity have considerable influence on yield. Further, HA-GGE biplot analysis identified Sagar, New Delhi, Sriganganagar, Durgapura, Warangal, Srinagar, Kanpur, and Mohanpur as the ideal testing environments, which demonstrated high efficiency in the selection of new genotypes with wider adaptability. Copyright � 2022 Parihar, Gupta, Hazra, Lamichaney, Sen Gupta, Singh, Kumar, Singh, Vaishnavi, Jaberson, Das, Dev, Yadav, Jamwal, Choudhary, Khedar, Prakash, Dikshit, Panwar, Katiyar, Kumar, Mahto, Borah, Singh, Das, Patil, Nanda, Kumar, Rajput, Chauhan, Patel, Kanwar, Kumar, Mishra, Kumar, Swarup, Mogali, Kumaresan, Manivannan, Gowda, Pandiyan, Rao, Shivani, Prusti, Mahadevu, Iyanar and Das.Publication Physiological and biochemical effect of elevated night temperature stress on bread wheat (Triticum aestivum L.)(United Arab Emirates University, 2023) Ahmad, Md. Afjal; Prakash, Pravin; Dujeshwer, K.; Srivastava, J.P.; Singh, Anil K.; Jatav, H.S.A laboratory experimentation was done in the Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University to evaluate elevated night temperature effect in wheat (Triticum aestivum L.) to see the response in some antioxidant and biochemical parameters in two wheat cultivars viz. HUW-234 (comparatively tolerant to heat stress) and sonalika (comparatively susceptible to heat stress). A significance difference was recorded in the parameters pertaining to antioxidant system includes catalase, SOD and APX activity were found to increase substantially when the plants kept under stress conditions (250C/240C day and night temperature) than the plants kept under controlled condition (250C/150C day and night temperature). The percent increase in the antioxidant enzyme activity was found to be more in the tolerant genotype (HUW-234) than in the susceptible genotype (Sonalika). There was 23.52 % increase was reported in the catalase activity by tolerant genotype when kept under stress conditions than those kept under control. Significant reductions were reported in most of the photosynthetic and membrane parameters viz., chlorophyll �a�, chlorophyll �b� and membrane thermostability index. Similar percent reduction was reported with respect to biochemical parameters (total protein content, starch content and soluble sugar content) in the susceptible variety kept under high night temperature regime. Based on the pooled data of two-year experimentation it can be concluded that variety HUW-234 was found to be more tolerant than variety sonalika to HNT stress. � (2023), (United Arab Emirates University). All Rights Reserved.Publication Postharvest Application of 6-Benzylaminopurine Preserves Quality and Delays Senescence of Pointed Gourd (Trichosanthes dioica Roxb.) Fruit(Springer, 2022) Yadav, Nitin; Singh, Anil K.; Pal, Akhilesh Kumar; Sharma, Swati; Barman, KalyanThis experiment investigated the effect of postharvest 6-benzylaminopurine treatment for preserving quality and improving storability of pointed gourd fruit. The fruits were treated with 6-benzylaminopurine (1.0�mM, 2.0�mM and 3.0�mM) by immersion method and stored at room temperature while, control fruits were treated with distilled water. Weight loss, total chlorophyll, total carotenoids, malondialdehyde content, ascorbic acid, total phenol and total flavonoids content were monitored at every 2�days of interval during storage. Among the treatments, 3.0�mM BAP-treated fruits showed 24% lower weight loss and 30% lower chlorophyll loss over control. After 6�days of storage, these fruits retained about 21% higher ascorbic acid, 27% higher total phenol and 28% higher total flavonoids than control. Senescence of the treated fruit was also delayed by 3.0�mM 6-benzylaminopurine treatment, showing 22% lower accumulation of malondialdehyde and 26% lower total carotenoids content compared to control. � 2021, The National Academy of Sciences, India.Publication Salicylic Acid Treatment Reduces Lipid Peroxidation and Chlorophyll Degradation and Preserves Quality Attributes of Pointed Gourd Fruit(Hindawi Limited, 2022) Yadav, Nitin; Singh, Anil K.; Emran, Talha Bin; Chaudhary, Ratiram G.; Sharma, Rohit; Sharma, Swati; Barman, KalyanThe marketability of pointed gourd fruit is drastically reduced after harvest due to moisture loss, chlorophyll degradation, yellowing of the skin, and shriveling. The present investigation studied the effect of exogenous salicylic acid (SA) treatment on senescence and fruit quality attributes of pointed gourd during storage under ambient conditions. Fruits were treated by immersing them in different concentrations of SA (1.0 mM, 2.0 mM, and 3.0 mM) and distilled water (control) for 5 minutes. The investigation showed beneficial effects of 3.0 mM SA treatment in lowering weight loss (16.8%), maintaining higher chlorophyll (32.8%) in the skin, and reducing lipid peroxidation (20.2%) compared to the control. SA (3.0 mM)-treated fruits retained 15.3% higher ascorbic acid and about 18% higher total phenol, flavonoids, and radical scavenging activity over pointed gourd fruits in the control group. However, significant difference in the total antioxidant capacity after 6 days of storage was not noted between SA-treated and control fruit. Thus, postharvest salicylic acid treatment can beneficially be used to extend marketability and delay quality deterioration of pointed gourd fruits stored under ambient conditions. � 2022 Nitin Yadav et al.
