Browsing by Author "Singh, Dhiraj Kumar"

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    Spatiotemporal Vegetation Variability and Linkage with Snow-Hydroclimatic Factors in Western Himalaya Using Remote Sensing and Google Earth Engine (GEE)
    (Multidisciplinary Digital Publishing Institute (MDPI), 2023) Singh, Dhiraj Kumar; Singh, Kamal Kant; Petropoulos, George P.; Boaz, Priestly Shan; Jain, Prince; Singh, Sartajvir; Gupta, Dileep Kumar; Sood, Vishakha
    The mountain systems of the Himalayan regions are changing rapidly due to climatic change at a local and global scale. The Indian Western Himalaya ecosystem (between the tree line and the snow line) is an underappreciated component. Yet, knowledge of vegetation distribution, rates of change, and vegetation interactions with snow-hydroclimatic elements is lacking. The purpose of this study is to investigate the linkage between the spatiotemporal variability of vegetation (i.e., greenness and forest) and related snow-hydroclimatic parameters (i.e., snow cover, land surface temperature, Tropical Rainfall Measuring Mission (TRMM), and Evapotranspiration (ET)) in Himachal Pradesh (HP) Basins (i.e., Beas, Chandra, and Bhaga). Spatiotemporal variability in forest and grassland has been estimated from MODIS land cover product (MCD12Q1) using Google Earth Engine (GEE) for the last 19 years (2001�2019). A significant inter- and intra-annual variation in the forest, grassland, and snow-hydroclimatic factors have been observed during the data period in HP basins (i.e., Beas, Chandra, and Bhaga basin). The analysis demonstrates a significant decrease in the forest cover (214 ha/yr.) at the Beas basin; however, a significant increase in grassland cover is noted at the Beas basin (459 ha/yr.), Chandra (176.9 ha/yr.), and Bhaga basin (9.1 ha/yr.) during the data period. Spatiotemporal forest cover loss and gain in the Beas basin have been observed at ~7504 ha (6.6%) and 1819 ha (1.6%), respectively, from 2001 to 2019. However, loss and gain in grassland cover were observed in 3297 ha (2.9%) and 10,688 ha (9.4%) in the Beas basin, 1453 ha (0.59%) and 3941 ha (1.6%) in the Chandra basin, and 1185 ha (0.92%) and 773 ha (0.60%) in the Bhaga basin, respectively. Further, a strong negative correlation (r = ?0.65) has been observed between forest cover and evapotranspiration (ET). However, a strong positive correlation (r = 0.99) has been recorded between grassland cover and ET as compared to other factors. The main outcome of this study in terms of spatiotemporal loss and gain in forest and grassland shows that in the Bhaga basin, very little gain and loss have been observed as compared to the Chandra and Beas basins. The present study findings may provide important aid in the protection and advancement of the knowledge gap of the natural environment and the management of water resources in the HP Basin and other high-mountain regions of the Himalayas. For the first time, this study provides a thorough examination of the spatiotemporal variability of forest and grassland and their interactions with snow-hydroclimatic factors using GEE for Western Himalaya. � 2023 by the authors.
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    Xenodiagnosis to evaluate the infectiousness of humans to sandflies in an area endemic for visceral leishmaniasis in Bihar, India: a transmission-dynamics study
    (Elsevier Ltd, 2021) Singh, Om Prakash; Tiwary, Puja; Kushwaha, Anurag Kumar; Singh, Shakti Kumar; Singh, Dhiraj Kumar; Lawyer, Phillip; Rowton, Edgar; Chaubey, Rahul; Singh, Abhishek Kumar; Rai, Tulika Kumari; Fay, Michael P; Chakravarty, Jaya; Sacks, David; Sundar, Shyam
    Background: Visceral leishmaniasis, also known on the Indian subcontinent as kala-azar, is a fatal form of leishmaniasis caused by the protozoan parasite Leishmania donovani and transmitted by the bites of the vector sandfly Phlebotomus argentipes. To achieve and sustain elimination of visceral leishmaniasis, the transmission potential of individuals exposed to L donovani from across the infection spectrum needs to be elucidated. The aim of this study was to evaluate the relative infectiousness to the sandfly vector of patients with visceral leishmaniasis or post-kala-azar dermal leishmaniasis, before and after treatment, and individuals with asymptomatic infection. Methods: In this prospective xenodiagnosis study done in Muzaffarpur district of Bihar, India, we included patients with clinically confirmed active visceral leishmaniasis or post-kala-azar dermal leishmaniasis who presented to the Kala-Azar Medical Research Center. These participants received treatment for L donovani infection. We also included asymptomatic individuals identified through a serosurvey of 17 254 people living in 26 high-transmission clusters. Eligible participants were aged 12�64 years, were HIV negative, and had clinically or serologically confirmed L donovani infection. During xenodiagnosis, the forearms or lower legs of participants were exposed to 30�35 female P argentipes sandflies for 30 min. Blood-engorged flies were held in an environmental cabinet at 28�C and 85% humidity for 60�72 h, after which flies were dissected and evaluated for L donovani infection by microscopy and quantitative PCR (qPCR). The primary endpoint was the proportion of participants with visceral leishmaniasis or post-kala-azar dermal leishmaniasis, before and after treatment, as well as asymptomatic individuals, who were infectious to sandflies, with a participant considered infectious if promastigotes were observed in one or more individual flies by microscopy, or if one or more of the pools of flies tested positive by qPCR. Findings: Between July 12, 2016, and March 19, 2019, we recruited 287 individuals, including 77 with active visceral leishmaniasis, 26 with post-kala-azar dermal leishmaniasis, and 184 with asymptomatic infection. Of the patients with active visceral leishmaniasis, 42 (55%) were deemed infectious to sandflies by microscopy and 60 (78%) by qPCR before treatment. No patient with visceral leishmaniasis was found to be infectious by microscopy at 30 days after treatment, although six (8%) were still positive by qPCR. Before treatment, 11 (42%) of 26 patients with post-kala-azar dermal leishmaniasis were deemed infectious to sandflies by microscopy and 23 (88%) by qPCR. Of 23 patients who were available for xenodiagnosis after treatment, one remained infectious to flies by qPCR on the pooled flies, but none remained positive by microscopy. None of the 184 asymptomatic participants were infectious to sandflies. Interpretation: These findings confirm that patients with active visceral leishmaniasis and patients with post-kala-azar dermal leishmaniasis can transmit L donovani to the sandfly vector and suggest that early diagnosis and treatment could effectively remove these individuals as infection reservoirs. An important role for asymptomatic individuals in the maintenance of the transmission cycle is not supported by these data. Funding: Bill & Melinda Gates Foundation. � 2021 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license