Browsing by Author "Tiwari, Harshita"

Now showing 1 - 5 of 5
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    Epigenetic manipulation for secondary metabolite activation in endophytic fungi: current progress and future directions
    (Taylor and Francis Ltd., 2023) Verma, Ashish; Tiwari, Harshita; Singh, Swati; Gupta, Priyamvada; Rai, Nilesh; Kumar Singh, Santosh; Singh, Bhim Pratap; Rao, Sombir; Gautam, Vibhav
    Fungal endophytes have emerged as a promising source of secondary metabolites with significant potential for various applications in the field of biomedicine. The biosynthetic gene clusters of endophytic fungi are responsible for encoding several enzymes and transcriptional factors that are involved in the biosynthesis of secondary metabolites. The investigation of fungal metabolic potential at genetic level faces certain challenges, including the synthesis of appropriate amounts of chemicals, and loss of the ability of fungal endophytes to produce secondary metabolites in an artificial culture medium. Therefore, there is a need to delve deeper into the field of fungal genomics and transcriptomics to explore the potential of fungal endophytes in generating secondary metabolites governed by biosynthetic gene clusters. The silent biosynthetic gene clusters can be activated by modulating the chromatin structure using chemical compounds. Epigenetic modification plays a significant role by inducing cryptic gene responsible for the production of secondary metabolites using DNA methyl transferase and histone deacetylase. CRISPR-Cas9-based genome editing emerges an effective tool to enhance the production of desired metabolites by modulating gene expression. This review primarily focuses on the significance of epigenetic elicitors and their capacity to boost the production of secondary metabolites from endophytes. This article holds the potential to rejuvenate the drug discovery pipeline by introducing new chemical compounds. � 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
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    Exploration of in vitro cytotoxic and in ovo antiangiogenic activity of ethyl acetate extract of Penicillium oxalicum
    (John Wiley and Sons Inc, 2023) Verma, Ashish; Rai, Nilesh; Gupta, Priyamvada; Singh, Swati; Tiwari, Harshita; Chauhan, Shashi Bhushan; Kailashiya, Vikas; Gautam, Vibhav
    Fungal endophytes have established new paradigms in the area of biomedicine due to their ability to produce metabolites of pharmacological importance. The present study reports the in vitro cytotoxic and in ovo antiangiogenic activity of the ethyl acetate (EA) extract of Penicillium oxalicum and their chemical profiling through Gas Chromatography�Mass Spectrometry analysis. Treatment of the EA extract of P. oxalicum to the selected human breast cancer cell lines (MDA-MB-231 and MCF-7) leads to the reduced glucose uptake and increased nitric oxide production suggesting the cytotoxic activity of EA extract of P. oxalicum. Our results further show that�treatment of EA extract of P. oxalicum attenuates the colony number, cell migration ability and alters nuclear morphology in both the human breast cancer cell lines. Furthermore, the treatment of EA extract of P. oxalicum mediates apoptosis by increasing the�expression of BAX, P21, FADD, and CASPASE-8 genes, with increased Caspase-3 activity. Additionally, in ovo chorioallantoic membrane (CAM) assay showed that the treatment of EA extract of P. oxalicum leads to antiangiogenic activity with perturbed formation of blood vessels. Overall, our findings suggest that the EA extract of P. oxalicum show in vitro cytotoxic and antiproliferative activity against human breast cancer cell lines, and in ovo antiangiogenic activity in CAM model. � 2023 Wiley Periodicals LLC.
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    On injective objects and existence of injective hulls in Q-TOP/(Y,?)
    (Shahid Beheshti University, 2022) Tiwari, Harshita; Srivastava, Rekha
    In this paper, motivated by Cagliari and Mantovani, we have obtained a characterization of injective objects (with respect to the class of embeddings in the category Q-TOP of Q-topological spaces) in the comma category Q-TOP/(Y,?), when (Y,?) is a stratified Q-topological space, with the help of their T0-reflection. Further, we have proved that for any Q-topological space (Y,?), the existence of an injective hull of ((X,?),f) in the comma category Q-TOP/(Y,?) is equivalent to the existence of an injective hull of its T0-reflection ((� X,� ?),� f ) in the comma category Q-TOP/(Y�, ?� ) (and in the comma category Q-TOP0/(Y�,?� ), where Q-TOP0 denotes the category of T0-Q-topological spaces) � Shahid Beheshti University
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    Recent Advancements in the Formulation of Nanomaterials-Based Nanozymes, Their Catalytic Activity, and Biomedical Applications
    (American Chemical Society, 2023) Singh, Swati; Rai, Nilesh; Tiwari, Harshita; Gupta, Priyamvada; Verma, Ashish; Kumar, Rajiv; Kailashiya, Vikas; Salvi, Prafull; Gautam, Vibhav
    Nanozymes are nanoparticles with intrinsic enzyme-mimicking properties that have become more prevalent because of their ability to outperform conventional enzymes by overcoming their drawbacks related to stability, cost, and storage. Nanozymes have the potential to manipulate active sites of natural enzymes, which is why they are considered promising candidates to function as enzyme mimetics. Several microscopy- and spectroscopy-based techniques have been used for the characterization of nanozymes. To date, a wide range of nanozymes, including catalase, oxidase, peroxidase, and superoxide dismutase, have been designed to effectively mimic natural enzymes. The activity of nanozymes can be controlled by regulating the structural and morphological aspects of the nanozymes. Nanozymes have multifaceted benefits, which is why they are exploited on a large scale for their application in the biomedical sector. The versatility of nanozymes aids in monitoring and treating cancer, other neurodegenerative diseases, and metabolic disorders. Due to the compelling advantages of nanozymes, significant research advancements have been made in this area. Although a wide range of nanozymes act as potent mimetics of natural enzymes, their activity and specificities are suboptimal, and there is still room for their diversification for analytical purposes. Designing diverse nanozyme systems that are sensitive to one or more substrates through specialized techniques has been the subject of an in-depth study. Hence, we believe that stimuli-responsive nanozymes may open avenues for diagnosis and treatment by fusing the catalytic activity and intrinsic nanomaterial properties of nanozyme systems. � 2023 American Chemical Society.
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    Recent Advances in Nanomaterials-Based Targeted Drug Delivery for Preclinical Cancer Diagnosis and Therapeutics
    (Multidisciplinary Digital Publishing Institute (MDPI), 2023) Tiwari, Harshita; Rai, Nilesh; Singh, Swati; Gupta, Priyamvada; Verma, Ashish; Singh, Akhilesh Kumar; Kajal; Salvi, Prafull; Singh, Santosh Kumar; Gautam, Vibhav
    Nano-oncology is a branch of biomedical research and engineering that focuses on using nanotechnology in cancer diagnosis and treatment. Nanomaterials are extensively employed in the field of oncology because of their minute size and ultra-specificity. A wide range of nanocarriers, such as dendrimers, micelles, PEGylated liposomes, and polymeric nanoparticles are used to facilitate the efficient transport of anti-cancer drugs at the target tumor site. Real-time labeling and monitoring of cancer cells using quantum dots is essential for determining the level of therapy needed for treatment. The drug is targeted to the tumor site either by passive or active means. Passive targeting makes use of the tumor microenvironment and enhanced permeability and retention effect, while active targeting involves the use of ligand-coated nanoparticles. Nanotechnology is being used to diagnose the early stage of cancer by detecting cancer-specific biomarkers using tumor imaging. The implication of nanotechnology in cancer therapy employs photoinduced nanosensitizers, reverse multidrug resistance, and enabling efficient delivery of CRISPR/Cas9 and RNA molecules for therapeutic applications. However, despite recent advancements in nano-oncology, there is a need to delve deeper into the domain of designing and applying nanoparticles for improved cancer diagnostics. � 2023 by the authors.