Browsing by Author "Yadav P.K."
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Item 2D material�based surface plasmon resonance biosensors for applications in different domains: an insight(Springer, 2024) Yadav P.K.; Kumar A.; Upadhyay S.; Kumar A.; Srivastava A.; Srivastava M.; Srivastava S.K.The design of surface plasmon resonance (SPR) sensors has been greatly enhanced in recent years by the advancements in the production and integration of nanostructures, leading to more compact and efficient devices. There have been reports of novel SPR sensors having distinct nanostructures, either as signal amplification tags like gold nanoparticles (AuNPs) or as sensing substrate-like two-dimensional (2D) materials including graphene, transition metal dichalcogenides (TMDCs), MXene, black phosphorus (BP), metal-organic frameworks (MOFs), and antimonene. Such 2D-based SPR biosensors offer advantages over conventional sensors due to significant increases in their sensitivity with a good figure of merit and limit of detection (LOD). Due to their atomically thin structure, improved sensitivity, and sophisticated functionalization capabilities, 2D materials can open up new possibilities in the field of healthcare, particularly in point-of-care diagnostics, environmental and food monitoring, homeland security protection, clinical diagnosis and treatment, and flexible or transient bioelectronics. The present study articulates an in-depth analysis of the most recent developments in 2D material�based SPR sensor technology. Moreover, in-depth research of 2D materials, their integration with optoelectronic technology for a new sensing platform, and the predicted and experimental outcomes of various excitation approaches are highlighted, along with the principles of SPR biosensors. Furthermore, the review projects the potential prospects and future trends of these emerging materials-based SPR biosensors to advance in clinical diagnosis, healthcare biochemical, and biological applications. Graphical abstract: (Figure presented.) � The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024.Item Antiproliferative Effects of Methanolic Fruit Extract of Solanum xenthocarpum (L.) on Human Breast Cancer Cells(John Wiley and Sons Inc, 2024) Maurya S.; Swati K.; Suvetha S.; Ghosh M.; Yadav P.K.Solanum xanthocarpum, a perennial herb native to India, contains steroidal glycoalkaloids with notable anticancer properties. This study investigated the antioxidant and antiproliferative effects of methanolic fruit extract of S. xanthocarpum on human breast cancer cells (MDA-MB-231). Phytochemical screening and LC-HRMS analysis confirmed presence of various primary and secondary metabolites. Antioxidant activity was assessed through DPPH, ABTS radical scavenging, reducing power, and phosphomolybdate assays. The extract demonstrated significant antioxidant potential with EC50 values of 60.10�0.88 ?g/mL (DPPH) and 392.29�3.93 ?g/mL (ABTS). Cytotoxicity against MDA-MB-231 cells was evaluated via morphological analysis, MTT assays, and IC50 determination (24.19�0.56 ?g/L). Apoptosis was confirmed using dual staining techniques (AO/EB, Hoechst 33342/PI, DAPI), revealing condensed nuclei, apoptotic bodies, and reduced mitochondrial membrane potential, as indicated by Rhodamine staining. Additionally, increased reactive oxygen species (ROS) levels were observed using H2-DCF-DA staining. The total phenolic and flavonoid contents of the extract were 127.78�3.547 mg GAE/g and 98.06�4.289 mg QE/g, respectively. These findings suggest that the methanolic fruit extract of S. xanthocarpum possesses strong antioxidant and anticancer activities, indicating its potential role in cancer treatment. Further studies are warranted to explore its bioactive compounds for developing novel anticancer therapies. � 2024 Wiley-VHCA AG, Zurich, Switzerland.Item Follicular oocyte as a potential target for severe acute respiratory syndrome coronavirus 2 infection(John Wiley and Sons Ltd, 2024) Yadav P.K.; Pandey A.N.; Premkumar K.V.; Tiwari M.; Pandey A.K.; Chaube S.K.Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was reported in December 2019 and rapidly became a pandemic as coronavirus disease 2019 (COVID-19). Apart from other organs, presence of specific receptor angiotensin-converting enzyme (ACE2) and corresponding proteases such as transmembrane serine protease 2, basigin and cysteine protease cathepsin L make follicular somatic cells as well as oocyte as potential targets for SARS-CoV-2 infection. The SARS-CoV-2 causes inflammation and hypoxia that generate reactive oxygen species (ROS) in critically ill patients. In addition, a large number of casualties and insecurity of life due to repeated waves of SARS-CoV-2 infection generate psychological stress and cortisol resulting in the further generation of ROS. The excess levels of ROS under physiological range cause meiotic instability, while high levels result in oxidative stress that trigger various death pathways and affect number as well as quality of follicular oocytes. Although, emerging evidence suggests that the SARS-CoV-2 utilises cellular machinery of ovarian follicular cells, generates ROS and impairs quality of follicular oocytes, the underlying mechanism of viral entry into host cell and its negative impact on the follicular oocyte remains poorly understood. Therefore, this review summarises emerging evidence on the presence of cellular machinery for SARS-CoV-2 in ovarian follicles and the potential negative impact of viral infection on the follicular oocytes that affect ovarian functions in critically ill and stressed women. � 2024 John Wiley & Sons Ltd.Item Gas hydrate saturation from NGHP 02 LWD data in the Mahanadi Basin(KeAi Communications Co., 2024) Shankar U.; Yadav P.K.; Devi S.; Yadav U.S.During the Indian National Gas Hydrate Program (NGHP) Expedition 02, Logging-while-drilling (LWD) logs were acquired at three sites (NGHP-02-11, NGHP-02-12, and NGHP-02-13) across the Mahanadi Basin in area A. We applied rock physics theory to available sonic velocity logs to know the distribution of gas hydrate at site NGHP-02-11 and NGHP-02-13. Rock physics modeling using sonic velocity at well location shows that gas hydrate is distributed mainly within the depth intervals of 150�265 m and 100�215 mbsf at site NGHP-02-11 and NGHP-02-13, respectively, with an average saturation of about 4% of the pore space and the maximum concentration of about 40% of the pore space at 250 m depth at site NGHP-02-11, and at site NGHP-02-13 an average saturation of about 2% of the pore space and the maximum concentration of about 20% of the pore space at 246 m depth, as gas hydrate is distributed mainly within 100�246 mbsf at this site. Saturation of gas hydrate estimated from the electrical resistivity method using density derived porosity and electrical resistivity logs from Archie's empirical formula shows high saturation compared to that from the sonic log. However, estimates of hydrate saturation based on sonic P-wave velocity may differ significantly from that based on resistivity, because gas and hydrate have higher resistivity than conductive pore fluid and sonic P-wave velocity shows strong effect on gas hydrate as a small amount of gas reduces the velocity significantly while increasing velocity due to the presence of hydrate. At site NGHP-02-11, gas hydrate saturation is in the range of 15%�30%, in two zones between 150-180 and 245�265 mbsf. Site NGHP-02-012 shows a gas hydrate saturation of 20%�30% in the zone between 100 and 207 mbsf. Site NGHP-02-13 shows a gas hydrate saturation up to 30% in the zone between 215 and 246 mbsf. Combined observations from rock physics modeling and Archie's approximation show the gas hydrate concentrations are relatively low (<4% of the pore space) at the sites of the Mahanadi Basin in the turbidite channel system. � 2023 Sinopec Petroleum Exploration and Production Research InstituteItem Green Carbon Quantum Dots for Efficient Sensing of Heavy Metal Ions(Springer Science and Business Media Deutschland GmbH, 2024) Yadav P.K.; Ganesan V.Owing to their unique fluorescence properties, carbon quantum dots (CQDs), the new zero-dimensional carbon nanomaterials have intrigued many research interests. CQDs have attracted massive attention worldwide because of their stunning properties like high solubility, high fluorescence intensity, biocompatibility, low toxicity, and easy surface functionalization. As a result, CQDs can cover various possible applications in many fields including catalysis, medical, light-emitting diodes, energy-related fields, and sensing. The selective and sensitive detection of heavy metal ions using CQDs involves exploiting the unique properties of these nanomaterials, particularly their fluorescence behavior. The use of green carbon quantum dots (CQDs) for detecting heavy metal ions is an area of growing interest due to the eco-friendly nature of the synthesis process and the unique properties of CQDs. Green synthesis typically involves using natural extracts, such as plant extracts or waste materials to produce CQDs. In this chapter, we will summarize and discuss the synthesis methods, surface passivation and functionalization, stability, mechanism of fluorescence sensing, and applications of CQDs for selective and sensitive detection of heavy metal ions. � The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2024.Item Influence of local defects in the generation of memory effect in Dy2Ti2O7 compound: Fe-doped study(Institute of Physics, 2024) Yadav P.K.; Upadhyay R.; Kumar R.; Nukala P.; Upadhyay C.The study of defect-induced effects in functional materials is high-priority research for the miniaturization of smart devices. To elucidate the influence of local defects stemming from intrinsic or extrinsic factors on the enigmatic behavior of cooperative spin dynamics, we investigated the Dy2-xFexTi2O7 compound within a narrow Fe substitution range (x = 0-0.15). Our experimental findings unequivocally demonstrate that the dynamic response of cooperative spins and the emergence of anomalous memory effect are intricately linked to local defects. Depending on the nature and dynamics of these defects, the dynamic response of cooperative spins undergoes alteration. Notably, observed anomalous effect becomes more pronounced in scenarios characterized by slower defect dynamics and when cooperative spins are further from equilibrium. The emergence and sensitivity of thermomagnetic hysteresis (memory effect) to cooperative spin dynamics and external stimuli underscore the richness of Dy2Ti2O7 as a material for captivating physics investigations. � 2024 IOP Publishing Ltd.Item l-Cystine-Based Polyurethane as a Drug-Delivery Vehicle in Targeted Cancer Therapy and Biomedical Applications(American Chemical Society, 2024) Bauri S.; Yadav P.K.; Choudhury A.M.; Maiti P.Controlled and sustained drug release is a critical aspect of drug-delivery systems (DDSs) that can be used in chemotherapy while ensuring therapy effectiveness and biosafety. Hence, polyurethane (PU) is modified using a biomolecule Cystine (CYS) for protracted drug release, aiming to enhance cancer treatment efficacy while minimizing adverse side effects in tumor patients. To confirm the formation of a polymer structure, characterization techniques such as NMR and FTIR are used, and the morphology is determined using SEM. Biocompatibility of the synthesized polymers is evaluated through cellular assessments, including MTT assay, cell adhesion, and antibacterial assay along with drug release using an anticancer drug, Paclitaxel (PTX). Notably, the incorporation of PTX in the polymer matrix results in minimal mortality (85% viable cells) rates in healthy cells (3T3), in contrast to a 56% mortality rate observed with the pure drug. While PTX shows a burst release and kills cancer cells only for the first 24 h, PU loaded with the drug shows sustained release and kills the cancer cells for 3 days. This vehicle selectively kills 59% of SiHA cells after a consecutive study of 3 days, which highlights the potential of this newly designed vehicle for effective drug delivery, particularly in anticancer treatments. Moreover, cystine�s antibacterial property adds up with PU; hence, PU shows antibacterial activity against Staphylococcus aureus (MIC, 20 ?g/mL) and also acts as a reductive oxygen species scavenger. Therefore, modifying PU with CYS has shown sustained release of PTX along with a selective effect on cells, underscoring its significance as a superior delivery agent and supported by a shred of convincing evidence. � 2024 American Chemical Society.Item Linear Model for Pore Pressure Predication in Gas Hydrate-bearing Sand Formation of Krishna-Godavari Basin (India) � A Case Study(Geological Society of India, 2024) Yadav P.K.; Shankar U.Pore pressure is a crucial geomechanical parameter, to decide the mud density while well drilling. The mud weight should be between formation pressure (pore pressure) and the fracture gradient of the reservoir rock, otherwise various kind of unwanted incidents like, kicks, blowout, breakout, well collapse, mud loss and lost circulation can happen; which can increase the cost of drilling and in worst case it may lead to a dangerous accident and consequently loss of life and assets at drilling site. In this study we are trying to find pore pressure within water bearing shale formations and gas hydrate (solid) bearing sand formation of four wells of Area B in the Krishna-Godavari Basin, namely, NGHP-02-17A, 19A, 22A, and 23A. Bower�s sonic, Eaton�s resistivity and Eaton�s sonic empirical equations have been used to estimate effective stress in the water-bearing shale formation. In gas hydrate-bearing sand formations these empirical equations cannot be useful for effective stress or pore pressure estimation and hence a linear model based on pressure-core data, relating effective stress and depth in meter below seafloor (mbsf) is generated. This model is applicable for the effective stress estimation in gas hydrate bearing sand formations of the Krishna-Godavari basin. The effective stress estimated from linear model and from core data are found in good agreement in all the wells. At site NGHP-02-23, in-situ pore-pressure was measured between depth of 270.38 to 271.38 mbsf with the help of modular dynamic formation tester (MDT) tool. The in-situ pore pressure (28.2 MPa) and estimated pore pressure (27.8 MPa) from the linear model are found in good agreement. Further, fracture pressures from Matthew-Kelly and Eaton�s method were estimated. The mud weight base on the reported mud density 1.3g/cc are found to higher than the pore pressures and lower than the fracture pressures and hence fulfil the criteria for safe drilling. � 2024 Geological Society of India, Bengaluru, India.Item Matrix metalloproteases as diagnostic and prognostic biomarkers in cancer(Elsevier, 2024) Maurya S.; Swati K.; Ghosh M.; Yadav P.K.Matrix metalloproteinases (MMPs) play a crucial role in the degradation and remodeling of the extracellular matrix. The role of MMPs in neoplastic cell invasion, metastasis and cancer progression has been studied extensively. There is a strong relationship between MMP overexpression, development of cancer, and clinical prognosis. Increased levels of one or more members of MMPs have been seen in the majority of malignancies. As a result, it has been anticipated that MMPs may act as both diagnostic and prognostic markers in cancer patients. Certain members of the MMP family have been confirmed as biomarkers of diverse human cancer types. MMPs and prognostic markers are two areas of cancer research that provide critical understanding of disease progression and patient outcomes. Their impact is widespread, from the management of metastatic disease to the early detection of cancer. Therefore, their clinical importance continues to propel advancements in precision medicine and cancer therapy. � 2025 Elsevier Inc. All rights are reserved including those for text and data mining AI training and similar technologies.Item Mitigating cypermethrin stress in Amaranthus hybridus L.: Efficacy of foliar-applied salicylic acid on growth, enzyme activity, and metabolite profiles(Elsevier B.V., 2024) Kumar A.; Yadav P.K.; Singh A.The study aimed to evaluate the role of foliar-applied salicylic acid (SA) (5-50 �M) in mitigating the effects of cypermethrin (CYP) stress on Amaranthus hybridus L., at its recommended (100 PPM) and double dose (200 PPM). CYP application at recommended dose proliferated the growth of the crop, while the double dose affected negatively. SA boosted the performance of the CYP-treated plants by reducing oxidative radical formation via increasing the activity of catalase, superoxide dismutase, peroxidase, ascorbate peroxidase, glutathione s-transferase, dehydroascorbate reductase, ascorbate, and proline. SA increased photosynthetic activity by increasing the leaf area, chlorophyll content and regulating the level of intracellular CO2, stomatal conductance, and transpiration in the CYP-treated plants. The fresh weight of the crop showed maximal increase with 10 �M SA (57 %) at recommended dose and with 20 �M SA (36.8 %) at double dose of CYP. Additionally, CYP residues showed maximum decline with 10 �M and 20 �M SA application in 100P and 200P plants, respectively. UHPLC-MS analysis showed increased levels of crucial secondary metabolites such as antheraxanthin, violaxanthin, kaempferol, rutin, cinnamic acid, ferulic acid, trigonelline, coumarin, scoparone, esculin, caryophyllene oxide, jasmonal with SA supplementation at 10 �M with 100P and at 20 �M with 200P treatments. The observed changes in enzyme activities, physiology and metabolite profiles underscore the potential of SA in mitigating pesticide-induced stress and enhancing plant growth and health. This study could further be explored in future by combining SA with other phytohormones and applying omics approaches could enhance crop resilience across diverse environments. � 2024Item Optimization of microwave parameters to enhance phytochemicals, antioxidants and metabolite profile of de-oiled rice bran(Nature Research, 2024) Chowdhury A.; Kumar A.Y.N.; Kumar R.; Maurya V.K.; Mahesh M.S.; Singh A.K.; Yadav P.K.; Ghosh M.The current study explores the effects of microwave treatment at varying wattage and durations on the phytoconstituents, antioxidant status, anti-nutritional factors (ANFs), and metabolite profiles of de-oiled rice bran. The total phenolics and flavonoids showed both increases and decreases depending on specific microwave parameters, while flavonol content consistently increased across all treated groups compared to the control. The DPPH and ABTS free radical scavenging activity, total antioxidant capacity, FRAP, CUPRAC, metal chelating activity, and ascorbic acid content were enhanced in most of the microwaved samples; however, longer microwave exposure at higher wattage led to their reduction. A treatment-specific decrease in ANFs, including condensed tannins, oxalates, and phytates, was observed. HRMS-based untargeted metabolomics identified a diverse range of primary and secondary metabolites, which clustered in a group-specific manner, indicating notable group-wise metabolite variations. Analysis of discriminating metabolites revealed no significant differences in the overall levels of phenolics, flavonoids, vitamins and cofactors, sugars, amino acids, terpenoids, fatty acids, and their derivatives among the treated groups compared to the control; however, several individual metabolites within these metabolite classes differed significantly. These findings suggest that optimized microwaving of de-oiled rice bran can enhance phytochemicals and antioxidants while improving the metabolite profile. � The Author(s) 2024.Item Reactive oxygen species signalling in the deterioration of quality of mammalian oocytes cultured in vitro: Protective effect of antioxidants(Elsevier Inc., 2024) Pandey A.N.; Yadav P.K.; Premkumar K.V.; Tiwari M.; Pandey A.K.; Chaube S.K.The in vitro fertilization (IVF) is the first choice of infertile couples worldwide to plan for conception. Besides having a significant advancement in IVF procedure, the success rate is still poor. Although several approaches have been tested to improve IVF protocol, minor changes in culture conditions, physical factors and/or drug treatment generate reactive oxygen species (ROS) in oocytes. Due to large size and huge number of mitochondria, oocyte is more susceptible towards ROS-mediated signalling under in vitro culture conditions. Elevation of ROS levels destabilize maturation promoting factor (MPF) that results in meiotic exit from diplotene as well as metaphase-II (M-II) arrest in vitro. Once meiotic exit occurs, these oocytes get further arrested at metaphase-I (M-I) stage or metaphase-III (M-III)-like stage under in vitro culture conditions. The M-I as well as M-III arrested oocytes are not fit for fertilization and limits IVF outcome. Further, the generation of excess levels of ROS cause oxidative stress (OS) that initiate downstream signalling to initiate various death pathways such as apoptosis, autophagy, necroptosis and deteriorates oocyte quality under in vitro culture conditions. The increase of cellular enzymatic antioxidants and/or supplementation of exogenous antioxidants in culture medium protect ROS-induced deterioration of oocyte quality in vitro. Although a growing body of evidence suggests the ROS and OS-mediated deterioration of oocyte quality in vitro, their downstream signalling and related mechanisms remain poorly understood. Hence, this review article summarizes the existing evidences concerning ROS and OS-mediated downstream signalling during deterioration of oocyte quality in vitro. The use of various antioxidants against ROS and OS-mediated impairment of oocyte quality in vitro has also been explored in order to increase the success rate of IVF during assisted reproductive health management. � 2024 Elsevier Inc.Item Spatially explicit simulation and forecasting of urban growth using weights of evidence based cellular automata model in a millennium city of India(Elsevier Ltd, 2024) Yadav P.K.; Mishra V.N.; Kumari M.; Kumar A.; Kumar P.; Bhatla R.The present study focuses on quantifying and simulating the future urban growth based on the land use/land cover (LULC) data created from the Landsat images of the year 1999, 2011, and 2022. These LULC maps help in analysing the expansion of urban areas over the years and forecast their potential growth in the future. The spatio-temporal processes of urban growth are quantified, and future patterns are simulated and forecasted using Weights of Evidence based Cellular Automata model built in Dinamica EGO (Environment for Geoprocessing Objects) platform. The process of urban growth was manifested through prominent contributing factors of infill expansion namely, distance to built-up areas, distance to main roads, population density, and public services etc. The model's performance was evaluated using Kappa statistics and the percentage of correct prediction (PCP) based two-way comparison method. For this purpose, the simulated map was first compared with the observed information of year 2022 using Kappa indices followed by the PCP value (90.40%) exhibiting high predictive ability of the model. These findings corroborate that the model can forecast the future urban growth scenarios effectively with reasonable accuracy. Based on the outcomes, the forecasting of future urban growth scenarios for years 2033 and 2044 was accomplished. Analysis of the LULC changes displays that urban land use will experience the highest increase. Growth in the study area is predicted to increase by 23.5% and 26.7% in year 2033 and 2044 respectively where new urban settlements can appear. The results demonstrated that an integrated geospatial model provides essential information about the pattern, simulation, and prediction of urban growth associated with various driving variables. � 2024 Elsevier Ltd
