Publication: Nanodiamonds activate blood platelets and induce thromboembolism
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Date
2014
Journal Title
Nanomedicine
Journal ISSN
Volume Title
Publisher
Future Medicine Ltd.
Abstract
Aim: Nanodiamonds (NDs) have been evaluated for a wide range of biomedical applications. Thus, thorough investigation of the biocompatibility of NDs has become a research priority. Platelets are highly sensitive and are one of the most abundant cell types found in blood. They have a central role in hemostasis and arterial thrombosis. In this study, we aim to investigate the direct and acute effects of carboxylated NDs on platelet function. Methods: In this study, pro-coagulant parameters such as platelet aggregability, intracellular Ca 2+ flux, mitochondrial transmembrane potential (ΔΨ m), generation of reactive oxygen species, surface exposure of phosphatidylserine, electron microscopy, cell viability assay and in vivo thromboembolism were analyzed in great detail. Results: Carboxylated NDs evoked significant activation of human platelets. When administered intravenously in mice, NDs were found to induce widespread pulmonary thromboembolism, indicating the remarkable thrombogenic potential of this nanomaterial. Conclusion: Our findings raise concerns regarding the putative biomedical applications of NDs pertaining to diagnostics and therapeutics, and their toxicity and prothrombotic properties should be critically evaluated. Original submitted 15 June 2012; Revised submitted 22 January 2013; Published online 30 April 201. © 2014 Future Medicine Ltd.
Description
Keywords
Integrin, Mitochondria, Nanodiamond, Platelet activation, Thromboembolism, Thrombus