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Size distribution analysis and physical/fluorescence characterization of graphene oxide sheets by flow cytometry

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dc.contributor.authorSingh, Sunil K.
dc.contributor.authorSingh, Manoj K.
dc.contributor.authorNayak, Manasa K.
dc.contributor.authorKumari, Sharda
dc.contributor.authorGrácio, José J.A.
dc.contributor.authorDash, Debabrata
dc.date.accessioned2025-03-03T09:09:39Z
dc.date.available2025-03-03T09:09:39Z
dc.date.issued2011
dc.description.abstractFlow cytometry is presented here as an alternative tool for analysis of size distribution and intrinsic fluorescence of graphene oxide (GO) sheets. Unlike other nanoparticles, GO sheets induced scatter signals reasonably distinct from the background noise of the system. The number of gated signals generated by the sheets was proportional to GO concentration, indicating that non-spherical particles like GO can be convincingly studied by flow cytometry. The asymmetry in forward scatter-side scatter contour plots was consistent with a non-uniform size distribution of individual sheets in the population, which contrasted with a prototype animal cell population. GO was endowed with intrinsic fluorescence detectable through the three fluorescence channels in the cytometer and allowed comparison of relative fluorescence intensities associated with individual sheets. Flow cytometry thus provides rapid access to high-dimension statistical information on individual GO sheets that is not achievable with existing characterization tools, and may prove to be an indispensable tool in graphene research. © 2010 Elsevier Ltd. All rights reserved.
dc.identifier.doihttps://doi.org/10.1016/j.carbon.2010.10.020
dc.identifier.issn86223
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/81176
dc.titleSize distribution analysis and physical/fluorescence characterization of graphene oxide sheets by flow cytometry
dc.typeArticle
dspace.entity.typePublication
journal.titleCarbon
journalvolume.identifier.volume49

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2011