Publication: Inducible periplasmic chromate reducing activity in Pseudomonas aeruginosa isolated from a leather tannery effluent
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Date
2001
Authors
Journal Title
Journal of Microbiology and Biotechnology
Journal ISSN
Volume Title
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Abstract
A chromate tolerant strain of Pseudomonas aeruginosa isolated from the effluent of a tannery showed significant enzymatic activity of chromate reduction. Cells grown in chromate-supplemented medium reduced 8 μg chromate/mg protein/h in the presence of NADH/NADPH. The chromate reducing activity was inducible as cells pregrown in chromate showed higher chromate reduction. In contrast, the periplasmic fraction of cells grown in chromate reduced 75% chromate in 4 h and the spheroplast fraction failed to do so, indicating that chromate reductase may be located in the periplasm. The presence of a 30 kDa protein in the periplasmic extracts of cells grown in the presence of chromate, but its absence of the protein in cells grown without chromate, points out a possible role of this protein in chromate reduction.
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Keywords
Chromate reductase, Inducibility, Periplasmic enzyme, Pseudomonas aeruginosa