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A new solid-state pH sensor and its application in the construction of all solid-state urea biosensor

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dc.contributor.authorPandey, P.C.
dc.contributor.authorUpadhyay, S.
dc.contributor.authorSingh, G.
dc.contributor.authorPrakash, R.
dc.contributor.authorSrivastava, R.C.
dc.contributor.authorSeth, P.K.
dc.date.accessioned2025-03-04T07:22:46Z
dc.date.available2025-03-04T07:22:46Z
dc.date.issued2000
dc.description.abstractA new solid-state pH sensor is developed using neutral poly(3-cyclohexyl thiophene) assembled over a Pt disk electrode. The new sensor is developed following two different approaches; 1) the neutral poly(3-cyclohexyl-thiophene) dissolved in chloroform and subsequent coating on to a Pt disk electrode; 2) the neutral polymer is incorporated into plasticized poly(vinyl chloride) matrix membrane. In both cases the polymer modified electrode is sensitive to pH and a reversible super Nernstian behavior is observed. The typical response of the pH sensor and its reversibility are reported. The polymer coated electrode is subsequently used to construct an all solid-state urea sensor. The construction of this new urea sensor involves the following two major steps; a) 20 μL of urease solution (40 mg/mL) is allowed to assemble overnight at 4 °C over neutral poly (3-cyclohexyl thiophene) modified electrode; b) an organically modified sol-gel layer is allowed to form over the urease adsorbed polymer modified electrode. The new solid-state urea sensor provides excellent reproducibility of the measurements and is stable for 3 months when stored at 4 °C under dry condition. The typical response of the solid-state urea sensor and the calibration plot of urea analysis are reported.
dc.identifier.doihttps://doi.org/10.1002/(SICI)1521-4109(200005)12:7<517
dc.identifier.issn10400397
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/89506
dc.publisherWiley-VCH Verlag
dc.subjectPoly(3-cyclohexyl thiophene)
dc.subjectSolid-state pH sensor
dc.subjectUrea biosensor
dc.titleA new solid-state pH sensor and its application in the construction of all solid-state urea biosensor
dc.typeArticle
dspace.entity.typePublication
journal.titleElectroanalysis
journalvolume.identifier.volume12

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