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Chemically characterized nanoencapsulated Homalomena aromatica Schott. essential oil as green preservative against fungal and aflatoxin B1 contamination of stored spices based on in vitro and in situ efficacy and favorable safety profile on mice

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dc.contributor.authorTiwari, Shikha
dc.contributor.authorUpadhyay, Neha
dc.contributor.authorSingh, Bijendra Kumar
dc.contributor.authorSingh, Vipin Kumar
dc.contributor.authorDubey, Nawal Kishore
dc.date.accessioned2025-01-27T10:23:53Z
dc.date.available2025-01-27T10:23:53Z
dc.date.issued2022
dc.description.abstractPresent study deals with the efficacy of nanoencapsulated Homalomena aromatica essential oil (HAEO) as a potent green preservative against toxigenic Aspergillus flavus strain (AF-LHP-NS 7), storage fungi, AFB1, and free radical-mediated deterioration of stored spices. GC�MS analysis revealed linalool (68.51%) as the major component of HAEO. HAEO was encapsulated into chitosan nanomatrix (CS-HAEO-Ne) and characterized through SEM, FTIR, and XRD. CS-HAEO-Ne completely inhibited A. flavus growth and AFB1 biosynthesis at 1.25 ?L/mL and 1.0 ?L/mL, respectively in comparison to unencapsulated HAEO (1.75 ?L/mL and 1.25 ?L/mL, respectively). CS-HAEO-Ne caused significant reduction in ergosterol content in treated A. flavus and provoked leakage of cellular ions (Ca+2, Mg+2, and K+) as well as 260 nm and 280 nm absorbing materials. Depletion of methylglyoxal level in treated A. flavus cells illustrated the novel antiaflatoxigenic efficacy of CS-HAEO-Ne. CS-HAEO-Ne exhibited superior antioxidant efficacy (IC50 (DPPH) = 4.5 ?L/mL) over unencapsulated HAEO (IC50 (DPPH) = 15.9 ?L/mL) and phenolic content. CS-HAEO-Ne depicted excellent in situ efficacy by inhibiting fungal infestation, AFB1 contamination, lipid peroxidation, and mineral loss with acceptable sensorial profile. Moreover, broad safety paradigm (LD50 value = 7150.11 mg/kg) of CS-HAEO-Ne also suggests its application as novel green preservative to enhance shelf life of stored spices. � 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
dc.identifier.doihttps://doi.org/10.1007/s11356-021-15794-2
dc.identifier.issn9441344
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/15237
dc.publisherSpringer Science and Business Media Deutschland GmbH
dc.subjectAflatoxin B<sub>1</sub>
dc.subjectChitosan
dc.subjectHomalomena aromatica essential oil
dc.subjectMethylglyoxal
dc.subjectNanoencapsulation
dc.titleChemically characterized nanoencapsulated Homalomena aromatica Schott. essential oil as green preservative against fungal and aflatoxin B1 contamination of stored spices based on in vitro and in situ efficacy and favorable safety profile on mice
dc.typeArticle
dspace.entity.typePublication
journal.titleEnvironmental Science and Pollution Research
journalvolume.identifier.volume29

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2022