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Recombinant YopJ induces apoptotic cell death in macrophages through TLR2

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dc.contributor.authorPandey, Ashok Kumar
dc.contributor.authorSodhi, Ajit
dc.date.accessioned2025-03-03T09:13:03Z
dc.date.available2025-03-03T09:13:03Z
dc.date.issued2011
dc.description.abstractBacterial species evolved evasive maneuvers to bypass their recognition by the receptors primarily TLRs of the innate immune cells. We have reported that 3 μg/ml of recombinant YopJ when provided extracellularly induced apoptosis in murine peritoneal macrophages in vitro. The present investigations demonstrate the role of TLR2 in apoptotic signals induced by rYopJ protein in murine peritoneal macrophages. The role of TLR2 in rYopJ induced macrophage apoptosis was shown by neutralization experiments and its co-immunoprecipitation with downstream molecule MyD88. The observed functional consequence of TLR2 neutralization were the inhibition of caspase-8 and caspase-3 activation, change in mitochondrial membrane potential (Δψm) and DNA fragmentation induced by rYopJ in macrophages. Further, rYopJ induced enhanced expression of IRAK-4, FADD, phosphorylation of IκB and p38 MAP kinase in macrophages. Pharmacological inhibitor of p38 MAP kinase and neutralization of TLR2 with neutralizing antibodies significantly inhibited the rYopJ induced caspases activation and DNA fragmentation, suggesting the possible involvement of TLR2 and p38 MAP kinase in rYopJ induced macrophages apoptosis. © 2010 Elsevier Ltd.
dc.identifier.doihttps://doi.org/10.1016/j.molimm.2010.07.018
dc.identifier.issn1615890
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/81413
dc.subjectApoptosis
dc.subjectRYopJ
dc.subjectToll-like receptor
dc.subjectYersinia pestis
dc.titleRecombinant YopJ induces apoptotic cell death in macrophages through TLR2
dc.typeArticle
dspace.entity.typePublication
journal.titleMolecular Immunology
journalvolume.identifier.volume48

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2011