Publication: Butylated hydroxytoluene and Butylated hydroxyanisole induced cyto-genotoxicity in root cells of Allium cepa L.
Loading...

Date
2021
Authors
Journal Title
Heliyon
Journal ISSN
Volume Title
Publisher
Elsevier Ltd
Abstract
This study aims to evaluate the effects of preservatives on somatic cells of Allium cepa. For the evaluation of cytotoxicity, root meristems of A. cepa were treated with 1000, 1500, 2000, 2500 ppm concentration for 5, 10, 15 days. The root tips showed concentration dependent growth retardation in all the used concentrations, as well as root texture, also has been changed mitotic index, total protein content decreased and frequency of chromosomal aberrations increased after 5 days treatment. Additionally, the RAPD-PCR method was applied to evaluate genotoxicity and found the highest concentration (2500 ppm) was comparatively more distant to the control group. Results concluded that BHT and BHA showed positive results and cytotoxic. � 2021
Description
Keywords
Allium cepa, Food preservatives, Mitotic index, RAPD-PCR, Root growth