Publication:
Bioprospecting of fungal endophytes from Oroxylum indicum (L.) Kurz with antioxidant and cytotoxic activity

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dc.contributor.authorRai, Nilesh
dc.contributor.authorKeshri, Priyanka Kumari
dc.contributor.authorGupta, Priyamvada
dc.contributor.authorVerma, Ashish
dc.contributor.authorKamble, Swapnil C.
dc.contributor.authorSingh, Santosh Kumar
dc.contributor.authorGautam, Vibhav
dc.date.accessioned2025-01-27T10:06:31Z
dc.date.available2025-01-27T10:06:31Z
dc.date.issued2022
dc.description.abstractOroxylum indicum (L.) Kurz, a medicinal plant, shows numerous pharmacological properties which may be attributed to the bioactive compounds produced by O. indicum or due to associated endophytes. In the present study, leaf of O. indicum was evaluated for the presence of associated fungal endophytes, and antioxidant and cytotoxic activities of bioactive compounds produced from them. Using culture-dependent approach, eight fungal endophytes belonging to five different genera were identified. Two endophytes Daldinia eschscholtzii and Ectophoma multirostrata have been reported for the first time from the leaf of O. indicum plant. High-performance thin-layer chromatography (HPTLC) of ethyl acetate (EA) extract of isolated fungal endophytes showed a distinct fingerprinting profile in EA extract of Colletotrichum gloeosporioides. Among identified endophytes, EA extract of C. gloeosporioides showed significant antioxidant activity against DPPH free radical, superoxide anion radical, nitric oxide radical and hydroxyl radical with EC50 values of 22.24�1.302 ?g/mL, 67.46 �0.576 ?g/mL, 80.10�0.706 ?g/mL and 61.55�1.360 ?g/mL, respectively. EA extract of C. gloeosporioides exhibited potential cytotoxicity against HCT116, HeLa and HepG2 cancer cell lines with IC50 values of 76.59 ?g/mL, 176.20 ?g/mL and 1750.70 ?g/mL, respectively. A comparative HPTLC fingerprinting and the antioxidant activity of C. gloeosporioides associated with two different hosts (leaf of O. indicum and dead twigs of other plant) showed that C. gloeosporioides produces bioactive compounds in a host-dependent manner. Copyright: � 2022 Rai et al.
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0264673
dc.identifier.issn19326203
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/14089
dc.publisherPublic Library of Science
dc.titleBioprospecting of fungal endophytes from Oroxylum indicum (L.) Kurz with antioxidant and cytotoxic activity
dc.typeArticle
dspace.entity.typePublication
journal.titlePLoS ONE
journalvolume.identifier.volume17

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