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Elucidation of antifungal and aflatoxin B1 inhibitory mode of action of Eugenia caryophyllata L. essential oil loaded chitosan nanomatrix against Aspergillus flavus

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dc.contributor.authorKujur, Anupam
dc.contributor.authorKumar, Akshay
dc.contributor.authorPrakash, Bhanu
dc.date.accessioned2025-01-30T06:08:54Z
dc.date.available2025-01-30T06:08:54Z
dc.date.issued2021
dc.description.abstractThe present study investigated the novel antifungal, and anti-aflatoxin B1 mechanism of Eugenia caryophyllata L. essential oil (ECEO) loaded chitosan nanomatrix against the toxigenic strain of A. flavus (AFLV-DK-02). Phytochemical profiling of ECEO was done by GC�MS which revealed eugenol (73.6%) as the primary bioactive compound. ECEO was encapsulated inside the chitosan nanomatrix (ECEO-Np) and characterized using SEM, AFM, FTIR and XRD analysis. The ECEO-Np exhibited enhance antifungal (0.25 ?L/mL) and anti-aflatoxin B1 inhibitory activity (0.15 ?L/mL) than ECEO. Antifungal and antiaflatoxin B1 inhibitory activity was found to be related with impairment in the biological functioning of the plasma membrane (ergosterol synthesis, leakage of membrane ions, UV light (260, 280 nm) absorbing material, dead cell by propidium iodide assay, mitochondrial membrane potential (MMP), methylglyoxal and inhibition in essential carbon substrate utilization). ECEO-Np exhibited remarkable free radical scavenging activity with IC50 value of 0.002 ?L/mL. ECEO-Np effectively preserves the sensory characteristics of exposed maize crop seed up to six months of storage and shows considerable safety profile (non-toxic, non-mutagenic, non-hepatotoxic, non-carcinogenic, non-tumorigenic and biodegradable) using computational ADMET (absorption, distribution, metabolism, excretion, and toxicity) analysis. � 2020 Elsevier Inc.
dc.identifier.doihttps://doi.org/10.1016/j.pestbp.2020.104755
dc.identifier.issn483575
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/25482
dc.publisherAcademic Press Inc.
dc.subjectAntifungal
dc.subjectChitosan nanomatrix
dc.subjectEugenia oil
dc.subjectMode of action
dc.subjectSafety profile
dc.titleElucidation of antifungal and aflatoxin B1 inhibitory mode of action of Eugenia caryophyllata L. essential oil loaded chitosan nanomatrix against Aspergillus flavus
dc.typeArticle
dspace.entity.typePublication
journal.titlePesticide Biochemistry and Physiology
journalvolume.identifier.volume172

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2021