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Responses of a hot spring cyanobacterium under ultraviolet and photosynthetically active radiation: photosynthetic performance, antioxidative enzymes, mycosporine-like amino acid profiling and its antioxidative potentials

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dc.contributor.authorAhmed, Haseen
dc.contributor.authorPathak, Jainendra
dc.contributor.authorRajneesh
dc.contributor.authorSonkar, Piyush K.
dc.contributor.authorGanesan, Vellaichamy
dc.contributor.authorH�der, Donat-P.
dc.contributor.authorSinha, Rajeshwar P.
dc.date.accessioned2025-01-30T06:16:40Z
dc.date.available2025-01-30T06:16:40Z
dc.date.issued2021
dc.description.abstractThis study summarizes the response of a hot spring cyanobacterium Fischerella sp. strain HKAR-14, under simulated light conditions of ultraviolet radiation (UVR), photosynthetically active radiation (PAR), PAR + UV-A (PA) and PAR + UV-A + UV-B (PAB). Exposure to UVR caused a decline in growth and Chl a while total carotene content increased under PA and PAB. Maximum photochemical efficiency of photosystem II (Fv/Fm) and relative electron transport rate decreased significantly in PA and PAB exposure. Higher non-photochemical quenching and lower photochemical quenching values were observed in UVR-exposed samples as compared to the control. Levels of intracellular reactive oxygen species (ROS) increased significantly in PAB and PA. Fluorescence microscopic images showed an increase in green fluorescence, indicating the generation of ROS in UVR. The antioxidant machinery including superoxide dismutase, catalase and peroxidase showed an increase of 1.76-fold and 2.5-fold superoxide dismutase, 2.4-fold and 3.7-fold catalase, 1.83-fold and 2.5-fold peroxidase activities under PA and PAB, respectively. High-performance liquid chromatography equipped with photodiode array detector, electrospray ionization mass spectrometry, Fourier-transform infrared spectroscopy and nuclear magnetic resonance spectroscopy analyses reveal the occurrence of a single mycosporine-like amino acid, shinorine (?max 332.3 � 2�nm, m/z 333.1), with a retention time of 1.157�min. The electrochemical characterization of shinorine was determined by cyclic voltammetry. The shinorine molecule possesses electrochemical activity and represents diffusion-controlled process in 0.1�M (pH 7.0) phosphate buffer. An antioxidant assay of shinorine showed its efficient activity as antioxidant which increased in a dose-dependent manner. � 2021, King Abdulaziz City for Science and Technology.
dc.identifier.doihttps://doi.org/10.1007/s13205-020-02562-1
dc.identifier.issn2190572X
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/26161
dc.publisherSpringer Science and Business Media Deutschland GmbH
dc.subjectAntioxidant
dc.subjectChlorophyll fluorescence
dc.subjectCyanobacteria
dc.subjectMycosporine-like amino acids
dc.subjectUltraviolet radiation
dc.titleResponses of a hot spring cyanobacterium under ultraviolet and photosynthetically active radiation: photosynthetic performance, antioxidative enzymes, mycosporine-like amino acid profiling and its antioxidative potentials
dc.typeArticle
dspace.entity.typePublication
journal.title3 Biotech
journalvolume.identifier.volume11

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