Publication: Flow Cytometry Approaches to Obtain Medulloblastoma Stem Cells from Bulk Cultures
Loading...
Date
2022
Authors
Journal Title
Methods in Molecular Biology
Journal ISSN
Volume Title
Publisher
Humana Press Inc.
Abstract
Cancer stem cells are considered the reservoir cancer cells that are resistant to most of the forms of cancer therapies and cause relapse of the tumor. Medulloblastoma (MB), a primary CNS tumor, is a very fast-growing tumor affecting younger population. In order to characterize medulloblastoma cancer stem cells or studying the drug resistance in MB mediated through the cancer stem cells, it becomes essential to isolate and study them. Isolation and characterization of tumor cells is a critical step in understanding the cancer progression and to devise novel approaches against them as drug targets. Typically, characterization of stem cells is done through surface marker analysis and with the advent of flow cytometry based techniques, this has become incredibly straightforward. Flow cytometry employs a uniformly linear flow of cells created by complex hydraulics of the flow cytometer followed by illuminating flow path with a LASER beam. This gives very valuable information about cell composition in forward scatter (FSC) and side scatter (SSC). The surface molecules of the cells can further be stained with various florescent dyes which upon excitation with the LASER beam will give the signal that will be detected by the instrument. Flow cytometer is high-throughput equipment and requires careful operation to get valuable information about the samples. In this chapter, we describe how from a bulk cell sample of medulloblastoma cells, cancer stem cells are isolated. � 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Description
Keywords
Bmi, Brain tumor initiating cells (BTICs), CD15, Flow cytometry (FACS) analysis, Immunostaining, Medulloblastoma (MB), Sox2