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Pongamol from the seeds of Tephrosia purpurea exert anti-cancer activity against cervical cancer cell line

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dc.contributor.authorKumari, Snehal
dc.contributor.authorChaudhary, Neha
dc.contributor.authorPriya, Khushbu
dc.contributor.authorRai, Geeta
dc.contributor.authorGautam, Dev Nath Singh
dc.contributor.authorSingh, Narendra Kumar
dc.date.accessioned2025-01-28T10:07:21Z
dc.date.available2025-01-28T10:07:21Z
dc.date.issued2023
dc.description.abstractTephrosia purpurea belongs to the family Fabaceae, is used for the treatment of inflammation, diabetes, cancer, chronic fever, boils, gingivitis etc. In the present study, three compounds (TP-1, TP-3 and TP-5) were isolated from ethyl acetate extract of the seeds of T. purpurea (EETP). Quantity of TP-3 (6.05%) in EETP was determined by HPLC. In-vitro anti-cancer activity of EETP and isolated compound TP-3 on SiHa cells as well as PBMCs (peripheral blood mononuclear cells) were evaluated by MTT (3-(4,5-di methyl thiazol-2-yl)-2,5-di phenyl tetrazolium bromide) assay. On the basis of spectroscopic analysis and physical properties, structure of isolated compounds TP-1, TP-3 and TP-5 were characterized as teclenone, pongamol and ?-sitosterol respectively. Isolated compound TP-3 showed cytotoxicity against SiHa cells with the IC50 33.06 �gmL-1. TP-3 showed significant cytotoxicity at the concentration range from 20-50 �gmL-1 as compared to control. Maximum cytotoxicity (72.75%) was observed at the concentration of 50 �gmL-1. TP-3 showed non-significant cytotoxicity against PBMCs cells at all the concentrations except at 50 �gmL-1 (cytotoxicity 24.19%). EETP showed significant cytotoxicity (IC50 value 113.63 �gmL-1) against SiHa cells at the concentration range from 75-150 �gmL-1 as compared to control. Maximum cytotoxicity (75.34%) was observed at the concentration of 150 �gmL-1. � 2023, National Institute of Science Communication and Policy Research. All rights reserved.
dc.identifier.doihttps://doi.org/10.56042/ijtk.v22i1.33137
dc.identifier.issn9725938
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/23663
dc.language.isoen
dc.publisherNational Institute of Science Communication and Policy Research
dc.subjectMTT assay
dc.subjectPBMCs cells
dc.subjectSiHa Cells
dc.subjectTeclenone
dc.subject?-sitosterol
dc.titlePongamol from the seeds of Tephrosia purpurea exert anti-cancer activity against cervical cancer cell line
dc.typeBook chapter
dspace.entity.typePublication
journal.titleIndian Journal of Traditional Knowledge
journalvolume.identifier.volume22

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