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Metabarcoding analysis of the bacterial succession during vermicomposting of municipal solid waste employing the earthworm Eisenia fetida

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dc.contributor.authorSrivastava, Vaibhav
dc.contributor.authorSquartini, Andrea
dc.contributor.authorMasi, Antonio
dc.contributor.authorSarkar, Abhijit
dc.contributor.authorSingh, Rajeev Pratap
dc.date.accessioned2025-01-30T06:04:33Z
dc.date.available2025-01-30T06:04:33Z
dc.date.issued2021
dc.description.abstractA culture-independent DNA metabarcoding analysis of the bacterial communities was carried out throughout a complete vermicomposting cycle of municipal solid waste material using the earthworm Eisenia fetida. 16S rRNA amplicons from the initial material (0 days), an intermediate (42 days), and a final stage (84 days) were sequenced in an Illumina NGS platform and compared. A steady increase in community diversity was observed corresponding to a 2.5-fold higher taxa richness and correspondingly risen values of the Shannon and Simpson ecological indexes and the evenness parameter. A total of 49,665 operational taxonomic units (OTUs) were counted. From the qualitative standpoint, a clear successional shift was observed with an initial community dominated by putatively plant-associated groups belonging to the Rhizobiales order within the Alphaproteobacteria class, regressively leaving the scores of relative abundance (RA) to the Firmicutes phylum and in particular to the Bacilli. Vermistabilization of municipal solid waste (MSW) increased (p < 0.001) the TKN and total P content in the final vermicompost, while pH, TOC, and C/N ratio declined (p < 0.001) in the process. Likewise, a progressive decrease was noticed in ?-glucosidase, acid phosphatase, and urease activity while protease and dehydrogenase showed a slight increase, followed by a steep fall. A strong positive correlation was observed among the canonical functions of physico-chemical attributes and enzyme activities. The canonical correspondence analysis (CCA) revealed that significant families did not change on the temporal scale; however, their abundance was influenced by the abiotic environmental factors. In comparison to prior studies on vermicomposting that used different earthworm species (Eisenia andrei) and different substrates, results reflect a considerable degree of substrate specificity for the earthworm species used. The results offer clues to optimize the vermistabilization of MSW along with its potential use in agriculture, to foster improved levels of the circular economy. � 2020 Elsevier B.V.
dc.identifier.doihttps://doi.org/10.1016/j.scitotenv.2020.144389
dc.identifier.issn489697
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/25180
dc.publisherElsevier B.V.
dc.subject16S
dc.subjectEisenia fetida
dc.subjectMetabarcoding
dc.subjectMunicipal solid waste (MSW)
dc.subjectVermicomposting
dc.titleMetabarcoding analysis of the bacterial succession during vermicomposting of municipal solid waste employing the earthworm Eisenia fetida
dc.typeArticle
dspace.entity.typePublication
journal.titleScience of the Total Environment
journalvolume.identifier.volume766

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2021