Publication:
Effect of chitosan seed priming on mungbean seedlings subjected to different levels of water potential

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Date

2023

Journal Title

Acta Physiologiae Plantarum

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Institute for Ionics

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Abstract

The main focus of our experiment was to understand the ameliorative effect of chitosan seed priming on different levels of water potential (water stress). Treatment with three levels of polyethylene glycol (PEG) induced water stress (?�0.3, ?�0.4, and ?�0.5�MPa) reduced seedling parameters, such as germination, mean germination time, shoot length, root length, fresh weight, dry weight, chlorophyll content, ?-amylase, and caused lipid peroxidation through increased MDA content. Seedlings treated with chitosan (0.15%) improved all the basic morpho-physiological attributes. Also, in seedlings subjected to stress condition, treatment with chitosan alleviated the water stress which is reflected through increased antioxidative system such as SOD and CAT, increased proline level, reduced MDA accumulation and increased activity of ?-amylase, offering strong defense mechanism, stress-protective metabolites, and better seed storage mobilization so as to facilitate seed germination ability and overall improved growth with higher tolerance to water stress. Meanwhile, the histochemical localization of H2O2 and superoxide radical O2? showed reduced accumulation of ROS in chitosan-treated leaves. The results indicated that chitosan treatment alleviated the adverse effects of water stress imposed at three levels of water potential. � 2022, The Author(s) under exclusive licence to Franciszek G�rski Institute of Plant Physiology, Polish Academy of Sciences, Krak�w.

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Keywords

Antioxidant, Chitosan priming, Mungbean, ROS, Water potential, Water stress

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