Publication: Role of white light in reversing UV-B-mediated effects in the N2-fixing cyanobacterium Anabaena BT2
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Date
2003
Journal Title
Journal of Photochemistry and Photobiology B: Biology
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The effects of various irradiances of artificial UV-B (280-315 nm) in the presence or absence of visible light (photosynthetically active radiation) on growth, survival, 14CO2 Uptake and ribulose 1,5-bisphosphate carboxylase (RuBISCO) activity were studied in the N2-fixing cyanobacterium Anabaena BT2. We tested the hypothesis whether or not visible radiation offers any protection against UV-B-induced deleterious effects on growth and photosynthesis in Anabaena BT2. Attempts were also made to determine the irradiances of UV-B where inhibitory effects could be mitigated by simultaneous irradiation with visible light. Exposure of cultures to 0.2 W m-2 or higher irradiance of UV-B caused inhibition of growth and survival and growth ceased above 1.0 W m-2. 14CO2 uptake and RuBISCO activity were found to be more sensitive to UV-B and around 60% reduction in 14CO2 uptake and RuBISCO activity occurred after exposure of cultures to 0.4 W m-2 for 1 h. However, growth, 14CO2 uptake and RuBISCO activity were nearly normal when UV-B (0.4 W m-2 ) and visible light (14A W m-2) were given simultaneously. Blue radiation (450 nm) was found to be the most effective in photoreactivation against UV-B, better than UV-A or any other light wavelength band. Our results demonstrate that the studied cyanobacterium possesses active photoreactivation mechanism(s) against UV-B-mediated damage which in turn probably allow survival under natural conditions in spite of being continuously exposed to the UV-B component present in the solar radiation. Continued growth of many algae and cyanobacteria in the presence of intense solar UV-B radiation under natural conditions seems to be due to the active role of photoreactivation. © 2003 Elsevier B.V. All rights reserved.
Description
Keywords
<sup>14</sup>CO<sub>2</sub> uptake, Anabaena BT2, Photoreactivation, RuBISCO activity, Survival, Ultraviolet radiation, Visible light