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Involvement of oxidative stress and role of antioxidative defense system in growing rice seedlings exposed to toxic concentrations of aluminum

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dc.contributor.authorSharma, Pallavi
dc.contributor.authorDubey, R.S.
dc.date.accessioned2025-03-04T05:01:31Z
dc.date.available2025-03-04T05:01:31Z
dc.date.issued2007
dc.description.abstractWhen seedlings of rice (Oryza sativa L.) cultivar Pant-12 were raised in sand cultures containing 80 and 160 μM Al3+ in the medium for 5-20 days, a regular increase in Al3+ uptake with a concomitant decrease in the length of roots as well as shoots was observed. Al3+ treatment of 160 μM resulted in increased generation of superoxide anion (O 2 -) and hydrogen peroxide (H2O2), elevated amount of malondialdehyde, soluble protein and oxidized glutathione and decline in the concentrations of thiols (-SH) and ascorbic acid. Among antioxidative enzymes, activities of superoxide dismutase (SOD EC 1.15.1.1), guaiacol peroxidase (Guaiacol POX EC 1.11.1.7), ascorbate peroxidase (APX EC 1.11.1.11), monodehydroascorbate reductase (MDHAR EC 1.6.5.4), dehydroascorbate reductase (EC 1.8.5.1) and glutathione reductase (EC 1.6.4.2) increased significantly, whereas the activities of catalase (EC EC 1.11.1.6) and chloroplastic APX declined in 160 μM Al3+ stressed seedlings as compared to control seedlings. The results suggest that Al3+ toxicity is associated with induction of oxidative stress in rice plants and among antioxidative enzymes SOD, Guaiacol POX and cytosolic APX appear to serve as important components of an antioxidative defense mechanism under Al3+ toxicity. PAGE analysis confirmed the increased activity as well as appearance of new isoenzymes of APX in Al3+ stressed seedlings. Immunoblot analysis revealed that changes in the activities of APX are due to changes in the amounts of enzyme protein. Similar findings were obtained when the experiments were repeated using another popular rice cv. Malviya-36. © 2007 Springer-Verlag.
dc.identifier.doihttps://doi.org/10.1007/s00299-007-0416-6
dc.identifier.issn7217714
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/86257
dc.subjectAluminum stress
dc.subjectAntioxidative enzymes
dc.subjectLipid peroxidation
dc.subjectOryza sativa
dc.subjectOxidative stress
dc.titleInvolvement of oxidative stress and role of antioxidative defense system in growing rice seedlings exposed to toxic concentrations of aluminum
dc.typeArticle
dspace.entity.typePublication
journal.titlePlant Cell Reports
journalvolume.identifier.volume26

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2007