Browsing by Author "Kushwaha A."

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    Suv39h1 Silencing Recovers Memory Decline in Scopolamine-Induced Amnesic Mouse Model
    (Springer, 2024) Kushwaha A.; Thakur M.K.
    Histone post-translational modifications play an important role in the regulation of long-term memory and modulation of expression of neuronal immediate early genes (IEGs). The lysine methyltransferase KMT1A/ Suv39h1 (a mammalian ortholog of the Drosophila melanogaster SU (VAR) 3-9) aids in the methylation of histone H3 at lysine 9. We previously reported that age-related memory decline is associated with an increase in Suv39h1 expression in the hippocampus of male mice. The scopolamine-induced amnesic mouse model is a well-known animal model of memory impairment. In the current study, we have made an attempt to find a link between the changes in the H3K9 trimethylation pattern and memory decline during scopolamine-induced amnesia. It was followed by checking the effect of siRNA-mediated silencing of hippocampal Suv39h1 on memory and expression of neuronal IEGs. Scopolamine treatment significantly increased global levels of H3K9me3 and Suv39h1 in the amnesic hippocampus. Suv39h1 silencing in amnesic mice reduced H3K9me3 levels at the neuronal IEGs (Arc and BDNF) promoter, increased the expression of Arc and BDNF in the hippocampus, and improved recognition memory. Thus, these findings suggest that the silencing of Suv39h1 alone or in combination with other epigenetic drugs might be effective for treating memory decline during amnesia. � 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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    Synthesis, Characterization and Electrical conductivities of the Complexes of Nickel(II), Copper(II) and Zinc(II) with 2,5-diamino-3,6-dichloro-1,4-benzoquinone
    (World Researchers Associations, 2024) Kushwaha A.; Smriti D.; Prasad R.L.
    Complexes of the type [M(dadb)] (M = Ni(II), Cu(II) and Zn(II)) with ligand 2,5-diamino-3,6-dichloro-1,4-benzoquinone (dadb) have been synthesized and characterized by microanalysis, magnectic susceptibility, electronic, IR, ESR spectroscopy techniques, thermogravimetric analysis (TG), differential thermal analysis (DTA), X-ray powder diffraction (XRD) and solid phase conductivity measurements. The room temperature ESR spectra of Cu(II) complex yield values, characteristics of square planar geometry. The solid state electrical conductivities of the complexes increase as the temperature increases from 311-383 K, with a band gap of 0.11- 0.21 eV, indicating their semiconducting behaviour. � 2024 World Researchers Associations. All rights reserved.