Browsing by Author "Maiti P."
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Item A non-covalently cross-linked self-healing hydrogel for drug delivery: characterization, mechanical strength, and anti-cancer potential(Royal Society of Chemistry, 2024) Jaiswal S.; Kumar S.; Yadav P.; Pal K.; Afgan S.; Acharya A.; Prakash R.; Maiti P.; Kumar R.This research article introduced a novel one-pot method for synthesizing hydrogels, utilizing iron ions (Fe3+) and guar gum succinate (GGS) as cross-linkers. These hydrogels were characterized as cross-linked networks, with hydrogen bonds forming a sacrificial network and coordination bonds serving as the primary network. The reversible nature of these networks was attributed to the hydrogels� exceptional toughness and remarkable self-healing properties. The hydrogel's chemical structure was confirmed through FTIR spectroscopy. XRD analysis highlighted the disruption of the crystalline nature of GGS upon cross-linking with Fe3+. By controlling the Fe3+ concentration, the hydrogels� mechanical properties were tailored. Rheological measurements demonstrated mechanical and self-healing properties, while swelling studies revealed pH-dependent behavior. In vitro studies showed the hydrogels� significant anti-proliferative effect against U-87MG (human glioblastoma) cancer cells, while remaining biocompatible with normal cell lines (HEK-293). These results indicated the potential application of these hydrogels in advancing cancer treatment strategies. � 2024 The Royal Society of Chemistry.Item Allylthiourea-mediated self-healing hydrogels based on poly(vinyl alcohol): Enhanced cell viability/biocompatibility and sustained drug release(John Wiley and Sons Inc, 2024) Yadav P.; Afgan S.; Pal K.; Jaiswal S.; Goswami P.; Prakash R.; Kumar R.; Koch B.; Maiti P.Hydrogel-based materials represent promising candidates for drug delivery. In our research, we synthesized a series of hydrogels�PVATU-1 (2:1), PVATU-2 (1:1), and PVATU-3 (0.5:1)�by adjusting the poly(vinyl alcohol) (PVA) ratio while maintaining a constant ratio of allylthiourea (ATU) monomer and glutaraldehyde cross-linker, aiming to understand their impacts on gelation. These PVATU hydrogels displayed impressive swelling in neutral conditions and exhibited visible self-healing capabilities. Notably, PVATU-1 shows superior mechanical strength among the variants. Surface analysis using AFM and SEM unveiled porous structures within the hydrogels, while thermal stability tests indicated their resilience up to 200�C. DSC analysis revealed minimal variation in glass transition temperatures (Tg), affirming stability. A distinct endothermic peak around 250�350�C across all hydrogels confirmed their semi-crystalline nature, corroborated by powder XRD. Noteworthy, PVATU-1 demonstrated optimal loading and releasing efficiency for levofloxacin, with 74.5% loading and 82.8% releasing efficiency within 18 h. Biocompatibility assessments on HeLa cells affirmed the nontoxicity of PVATU hydrogel treatments. � 2024 Wiley Periodicals LLC.Item Doxorubicin-Intercalated Li-Al-Based LDHs as Potential Drug Delivery Nanovehicle with pH-Responsive Therapeutic Cargo for Tumor Treatment(American Chemical Society, 2024) Maity S.; Dubey D.K.; Meena J.; Shekher A.; Singh R.S.; Maiti P.Clinical oncology is currently experiencing a technology bottleneck due to the expeditious evolution of therapy defiance in tumors. Although drugs used in chemotherapy work for a sort of cell death with potential clinical application, the effectiveness of chemotherapy-inducing drugs is subject to several endogenous conditions when used alone, necessitating the urgent need for controlled mechanisms. A tumor-targeted drug delivery therapy using Li-Al (M+/M3+)-based layered double hydroxide (LDHs) family has been proposed with the general chemical formula [M+1-x M3+x (OH)]2x+[(Am-)2x/m. n(H2O)]2x?, which is fully biodegradable and works in connection with the therapeutic interaction between LDH nanocarriers and anticancerous doxorubicin (DOX). Compositional variation of Li and Al in LDHs has been used as a nanoplatform, which provides a functional balance between circulation lifetime, drug loading capacity, encapsulation efficiency, and tumor-specific uptake to act as self-regulatory therapeutic cargo to be released intracellularly. First-principle analyses based on DFT have been employed to investigate the interaction of bonding and electronic structure of LDH with DOX and assess its capability and potential for a superior drug carrier. Following the internalization into cancer cells, nanoformulations are carried to the nucleus via lysosomes, and the mechanistic pathways have been revealed. Additionally, in vitro along with in vivo therapeutic assessments on melanoma-bearing mice show a dimensional effect of nanoformulation for better biocompatibility and excellent synergetic anticancer activity. Further, the severe toxic consequences associated with traditional chemotherapy have been eradicated by using injectable hydrogel placed just beneath the tumor site, and regulated release of the drug has been confirmed through protein expression applying various markers. However, Li-Al-based LDH nanocarriers open up new design options for multifunctional nanomedicine, which has intriguing potential for use in cancer treatment through sustained drug delivery. � 2024 American Chemical Society.Item Impact of chickpea aquafaba-based emulsion on the physicochemical, nutritional, rheological and structural characteristics of little millet (Panicum sumatrense Roth.) flour cake(Royal Society of Chemistry, 2024) Madhavi B.; Asaithambi N.; Kumar A.; Maiti P.; Chandra Rai D.; Duary R.K.Recent patterns in food consumption indicate that consumers have a strong desire for vegan, gluten-free, and healthy diets. This study investigates the utility of chickpea aquafaba and little millet flour (LMF) as alternatives to egg and refined flour, respectively, for developing egg-free and gluten-free cake recipes. The cake and batter were analyzed for their physicochemical, structural, sensorial, rheological and textural properties, with a shelf-life study conducted over a period of 12 days (30 � 2 �C). The batter's density (1.13 � 0.01 g cm?3) and viscosity (15796.7 � 0.09 cp) increased with the addition of LMF, and the batter predominantly exhibited an elastic behavior with G? > G?? for all cake formulations. These characteristic changes in cake batter caused the cake's weight to increase (by 10%) while causing its height, baking loss, volume, specific volume and symmetry index to decrease. The textural characteristics showed that adding LMF to cakes enhanced the hardness (145.00 � 9.45 N) and firmness (19.36 � 2.12 N) and decreased their cohesion, chewiness, gumminess, springiness and resilience. The image analysis showed more uniform bubble distribution in wheat flour (WF) cake than in LMF cakes, and an increase in LMF content resulted in a drop in cell circularity. The microbial degradation of cakes was observed from the 6th day of storage. LMF cake samples exhibited good texture and physical and sensory attributes comparable to those of the WF cake sample. Therefore, the study demonstrated the potential use of alternative ingredients, such as LMF and aquafaba emulsion, in the production of egg-free and gluten-free cakes. These ingredients could facilitate the scaling up of sustainable production practices for baked goods in the future. � 2025 RSC.Item l-Cystine-Based Polyurethane as a Drug-Delivery Vehicle in Targeted Cancer Therapy and Biomedical Applications(American Chemical Society, 2024) Bauri S.; Yadav P.K.; Choudhury A.M.; Maiti P.Controlled and sustained drug release is a critical aspect of drug-delivery systems (DDSs) that can be used in chemotherapy while ensuring therapy effectiveness and biosafety. Hence, polyurethane (PU) is modified using a biomolecule Cystine (CYS) for protracted drug release, aiming to enhance cancer treatment efficacy while minimizing adverse side effects in tumor patients. To confirm the formation of a polymer structure, characterization techniques such as NMR and FTIR are used, and the morphology is determined using SEM. Biocompatibility of the synthesized polymers is evaluated through cellular assessments, including MTT assay, cell adhesion, and antibacterial assay along with drug release using an anticancer drug, Paclitaxel (PTX). Notably, the incorporation of PTX in the polymer matrix results in minimal mortality (85% viable cells) rates in healthy cells (3T3), in contrast to a 56% mortality rate observed with the pure drug. While PTX shows a burst release and kills cancer cells only for the first 24 h, PU loaded with the drug shows sustained release and kills the cancer cells for 3 days. This vehicle selectively kills 59% of SiHA cells after a consecutive study of 3 days, which highlights the potential of this newly designed vehicle for effective drug delivery, particularly in anticancer treatments. Moreover, cystine�s antibacterial property adds up with PU; hence, PU shows antibacterial activity against Staphylococcus aureus (MIC, 20 ?g/mL) and also acts as a reductive oxygen species scavenger. Therefore, modifying PU with CYS has shown sustained release of PTX along with a selective effect on cells, underscoring its significance as a superior delivery agent and supported by a shred of convincing evidence. � 2024 American Chemical Society.Item Neurogenic and angiogenic poly(N-acryloylglycine)-co-(acrylamide)-co-(N-acryloyl-glutamate) hydrogel: preconditioning effect under oxidative stress and use in neuroregeneration(Royal Society of Chemistry, 2024) Wasnik K.; Gupta P.S.; Singh G.; Maity S.; Patra S.; Pareek D.; Kumar S.; Rai V.; Prakash R.; Acharya A.; Maiti P.; Mukherjee S.; Mastai Y.; Paik P.Traumatic injuries, neurodegenerative diseases and oxidative stress serve as the early biomarkers for neuronal damage and impede angiogenesis and subsequently neuronal growth. Considering this, the present work aimed to develop a poly(N-acryloylglycine)-co-(acrylamide)-co-(N-acryloylglutamate) hydrogel [p(NAG-Ac-NAE)] with angiogenesis/neurogenesis properties. As constituents of this polymer modulate their vital role in biological functions, inhibitory neurotransmitter glycine regulates neuronal homeostasis, and glutamatergic signalling regulates angiogenesis. The p(NAG-Ac-NAE) hydrogel is a highly branched, biodegradable and pH-responsive polymer with a very high swelling behavior of 6188%. The mechanical stability (G?, 2.3-2.7 kPa) of this polymeric hydrogel is commendable in the differentiation of mature neurons. This hydrogel is biocompatible (as tested in HUVEC cells) and helps to proliferate PC12 cells (152.7 � 13.7%), whereas it is cytotoxic towards aggressive cancers such as glioblastoma (LN229 cells) and triple negative breast cancer (TNBC; MDA-MB-231 cells) and helps to maintain the healthy cytoskeleton framework structure of primary cortical neurons by facilitating the elongation of the axonal pathway. Furthermore, FACS results revealed that the synthesized hydrogel potentiates neurogenesis by inducing the cell cycle (G0/G1) and arresting the sub-G1 phase by limiting apoptosis. Additionally, RT-PCR results revealed that this hydrogel induced an increased level of HIF-1? expression, providing preconditioning effects towards neuronal cells under oxidative stress by scavenging ROS and initiating neurogenic and angiogenic signalling. This hydrogel further exhibits more pro-angiogenic activities by increasing the expression of VEGF isoforms compared to previously reported hydrogels. In conclusion, the newly synthesized p(NAG-Ac-NAE) hydrogel can be one of the potential neuroregenerative materials for vasculogenesis-assisted neurogenic applications and paramount for the management of neurodegenerative diseases. � 2024 The Royal Society of Chemistry.Item Pyrene-tagged poly(N-vinyl pyrrolidone) as efficient nano-carrier for anticancer drug delivery(Taylor and Francis Ltd., 2024) Mitra K.; Maity S.; Hajra A.; Singh S.; Mondal S.; Singh J.; Maiti P.; Ray B.Herein, we report the use of self-assembled amphiphilic pyrene-tagged poly(N-vinyl pyrrolidone) (PyPNVP) micelles as effective anticancer delivery vehicle. Self-assembly phenomenon of PyPNVP has been studied in aqueous medium. Anticancer drug doxorubicin has been encapsulated in the hydrophobic micellar core and in�vitro release profiles have been explored in four different pH values (pH 7.4 ? 1.4). The drug-loaded construct has shown pH-responsive higher drug release at lower pH. Anticancer activity of the construct has been studied against human squamous cell carcinoma of the cervix uteri cell line. The in�vitro study has shown the highly effective tumoricidal activity. � 2023 Taylor & Francis Group, LLC.Item Rheological, Textural and Sensorial Properties of Quarg-Type Cheese Incorporated with Encapsulated Terminalia Arjuna(Elsevier Ltd, 2024) Aparnna V.P.; Chauhan A.K.; Singh S.; Prakash R.; Maiti P.This study aimed to investigate the possibility of enhancing the functionality of Quarg-type cheese by adding Arjuna bark powder in encapsulated form. Arjuna tree bark powder was extracted via ethanolic cold maceration. The encapsulated powder was made using Maltodextrin and Sodium alginate wall material solution and had encapsulating efficiency of 42 � 4.2%. Response Surface Methodology (RSM) was applied to conduct optimization, and subsequent trials were thoroughly investigated to assess their Physico-chemical, sensory, textural, and desirable attributes. The recommended levels of variables as suggested by the Response Surface Methodology (RSM) encompassed 3% of encapsulated Arjuna herb powder and 4% of honey. The conducted analysis has demonstrated that the inclusion of these variables has resulted in a notable improvement in the functionality of quarg-type cheese. Specifically, these variables have contributed to the enhancement of its antioxidant properties, sensory characteristics, rheological attributes, and textural qualities. The encapsulation of the Arjuna herb effectively concealed its astringent taste in the optimized product. Furthermore, the inclusion of honey not only improved the product's acceptability but also strengthened its antioxidative properties. � 2023 The Author(s)Item Solid-state synthesis of novel anticancer drug eutectic mixture; anticancer, physical and thermal studies(Elsevier B.V., 2024) Chaudhary S.; Rai R.N.; Maity S.; Maiti P.Green, solvent-free synthesis is adopted for a new pharmaceutical drug-drug eutectic with the utmost 100% yield. The synthesized eutectic mixture is significant for anticancer activity. The mandate of clinical tests could be avoided as selected nicotinamide, salicylamide, p-aminoacetinilide and benzanilide are known drugs. The compositional behaviors and established phase diagrams infer the formation of eutectics. The thermal behaviors of eutectics are studied using DSC. Further studies of eutectics are targeted for their biological applications, particularly for cell viability and anticancer activity against SiHa cancer cells where eutectic of benzanilide-salicylamide system infers its potential applicability for anticancer activity. � 2024Item Tissue-engineered vascular grafts for cardiovascular disease management: Current strategies, challenges, and future perspectives(John Wiley and Sons Inc, 2024) Das K.K.; Mishra Tiwari R.; Shankar O.; Maiti P.; Dubey A.K.Cardiovascular diseases are the leading cause of mortality which primarily occurs due to the blood vessel obstruction or narrowing. Surgical procedures such as, coronary artery and peripheral artery bypass grafting frequently require vascular grafts for long-term revascularization. However, using autogenous vessels, such as the internal thoracic artery and saphenous vein, especially for vessels with diameters less than 6 mm, are associated with number of concerns due to limited availability, invasive retrieval procedures, and aptness. To overcome these limitations, the development of tissue-engineered vascular grafts (TEVGs) is in continuous thrust. This review comprehensively provides the potentiality of a range of artificial and naturally occurring biopolymers and their fabrication techniques, cell sources and seeding techniques to realize the state-of-the-art TEVGs. Moreover, this review article presents a synopsis of insights obtained from a variety of in vitro and in vivo studies, including human clinical trials. It underscores the need for further exploration into key areas such as optimal cell sources, seeding techniques, mechanical properties, hemodynamics, graft integration, the impact of patient conditions, optimum burst pressure, sufficient suture strength, hydrophilicity, biodegradability, and related factors. In summary, the review offers insights into the current strategies, challenges, and future perspectives of TEVG. � 2024 The Author(s). MedComm � Biomaterials and Applications published by John Wiley & Sons Australia, Ltd on behalf of Sichuan International Medical Exchange & Promotion Association (SCIMEA).
