BHU Institutional Repository :: Browsing by Author "Mishra V.K."

Browsing by Author "Mishra V.K."

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A modified methodology for extraction and quantification of microplastics in soil
(Elsevier B.V., 2024) Gupta E.; Mishra V.K.; Patel A.; Srivastava P.K.
The ubiquitousness of microplastics (<5 mm) has become a pressing environmental concern globally due to the extensive use of plastics. Microplastics have been well-studied in aquatic environments but not well-characterized in soils. Present analytical processes to quantify microplastics accurately in soil samples are quite challenging and require improved and validated analytical steps to eliminate the obscurities and biases. We aimed to develop an effective method for the extraction and quantification of microplastics from soil samples. Different ratios of low-(NaCl) and high-density solutions (ZnCl2/ NaBr) were tested to determine the most efficient combination for density-dependent separation of microplastics from soil. The combination of low- (1:6) and high-density (1:3) solutions {as weight of soil(g)/volume of density solution(ml)} accounted for 95% recovery of the spiked microplastic particles from soil samples. Likewise, different soil-to-solution ratios of H2O2 were tested for the removal of soil organic matter with heating and non-heating steps. Prior removal of organic matter from soil samples achieved a clear supernatant that facilitated 99% recovery of microplastic particles. The validation of individually spiked microplastic particles of small (10-100 ?m) and large scale (100-5000 ?m) resulted in recovery ranging from 88 to 99%. A validated modified method with prior digestion followed by density-dependent separation was further tested using the field samples with microplastic contamination. The microplastics of different shapes, sizes, colours and polymeric compositions were reported efficiently and well characterized in the field-collected soil samples using this method. � 2024 Elsevier B.V.
Assessing the water quality of River Ganga in Varanasi, India, through WQI, NPI, and multivariate techniques: a comprehensive study
(IWA Publishing, 2024) Singh G.; Chaudhary S.; Gupta D.; Mishra V.K.
In the present research water quality from nine different sampling points (S1�S9) from the River Ganga at Varanasi was examined for different water quality parameters, and multivariate statistical analyses were carried out. Subsequently, several indices, such as water quality index and Nemerow pollution index (NPI), were calculated. The results indicated that the Ganga River at Varanasi had high levels of coliform concentrations, altered pH, and elevated dissolved oxygen/biochemical oxygen demand and chemical oxygen demand values. The weighted arithmetic water quality index values revealed that sites S8, S9, and S2 were the most polluted and unfit for bathing and drinking. Most of the sampling sites have NPI values greater than 1 for several parameters, indicating high levels of pollution. The study revealed that the water quality is poor for bathing and drinking at most of the sites throughout the year. In addition, the upstream water quality assessment revealed that water quality was good compared with the heavily contaminated downstream region. This knowledge can be useful for environmentalists, policymakers, and water resource managers to develop strategic plans to preserve the cultural and aesthetic worth of the Ganga River in the future. � 2024 The Authors.
Assessment of heavy metal pollution level, ecological and human health risks in surface water of Narmada River, India
(Springer Science and Business Media Deutschland GmbH, 2024) Gupta D.; Shukla R.; Srivastava P.K.; Mishra V.K.
The present research focuses on the evaluation of selected heavy metals (Al, Cr, Mn, Fe, Ni, Cu, Zn, Cd, & Pb) in the upper stretch of the Narmada River in central India to detect the pollution status, degree of heavy metals contamination, and threat to human health. The concentration of the selected heavy metals was in the order of Al > Fe > Zn > Mn > Cr > Ni > Cu > Pb > Cd and Al > Fe > Zn > Mn > Cr > Ni > Pb > Cu > Cd for pre-monsoon and post-monsoon season respectively. Further indices like the heavy metal evaluation index (HEI) indicated that the water of the Narmada River was medium polluted, heavy metal pollution index (HPI) categorized the water as moderately to heavily polluted. The nemerrow pollution index (NPI) classified 8% of sampling sites as low polluted, 42% as medium polluted, and 50% as highly polluted in pre-monsoon. Similarly, for the post-monsoon, NPI classified 42% of sampling sites as low polluted, 33% as medium polluted, and 36% as highly polluted water. Total hazard risk indicated a non-carcinogen risk for children and adults. In both seasons, based on human health risk assessment, ingestion of heavy metals contaminated water especially with Cr was identified to be highly prone to cancer risk. This research will aid in the development of effective planning and policies for enhancing river water quality and reducing ecological and human health risks. Graphical Abstract: (Figure presented.) � The Author(s), under exclusive licence to Springer Nature Switzerland AG 2024.
Base-Induced Annulation of Glycal-Derived ?-iodopyranone with 2-Aminopyrimidinones: Access to Chiral Imidazopyrimidinones
(John Wiley and Sons Inc, 2024) Mishra V.K.; Tiwari G.; Khanna A.; Yadav Y.; Sagar R.
A simple, environmentally benign and catalyst-free method for the synthesis of chirally enriched imidazo[1,2-a]pyrimidinone glycohybrids has been successfully developed. The protocol is based on a base-induced annulation of ?-iodo-pyranone with Michael addition of 2-aminopyrimidinones followed by intramolecular nucleophilic substitution reaction. The privilege of this method includes mild reaction conditions, eco-friendly solvent and a transition-metal-free approach. Moreover, using straightforward simple methods, this reaction method exhibits a broad range of substrate scope and remarkable tolerance toward various functional groups. � 2024 Wiley-VCH GmbH.
Characterization of groundwater potability and irrigation potential in Uttar Pradesh, India using water quality index and multivariate statistics
(IWA Publishing, 2024) Chaudhary S.; Singh G.; Gupta D.; Maunas S.S.; Mishra V.K.
This study includes groundwater quality data from 290 monitoring sites from 69 districts of Uttar Pradesh, India. The analysis of the data showed that 98.97, 24.48, 52.07, and 68.97% of groundwater samples had concentrations of electrical conductivity (EC), total hardness (TH), Mg2�, and HCO3-, respectively, higher than the maximum permissible limit. Groundwater quality index (GWQI) was calculated for these 290 monitoring sites which revealed that 21 sites (7.24%) had inappropriate GWQI for drinking water, and 18 sites (6.21%) had an unsuitable index for irrigation. Most of the sampling sites (98.97%) showed high EC contents in groundwater with a mean value of 999.33 ?S/cm. Fluoride content was found within the permissible limits in 95.52% of the samples, while 4.48% had high concentrations. The use of hierarchical cluster analysis differentiated all the sites into two clusters: one with high pollution and the other with low pollution. Significant correlations exist between physicochemical and irrigation indicators in the correlation matrix. High loadings of EC, TH, Ca2�, Mg2�, Na�, Cl-, and SO42- were identified in the first principal component, which are thought to be pollution-controlled processes from anthropogenic sources. According to the Chadha diagram, CaHCO3 and Ca�Mg�HCl were the two most prevalent chemicals in the water. � 2024 The Authors.
Copper-catalyzed synthesis of pyrazolo[1,5-a]pyrimidine based triazole-linked glycohybrids: mechanistic insights and bio-applications
(Nature Research, 2024) Tiwari G.; Khanna A.; Tyagi R.; Mishra V.K.; Narayana C.; Sagar R.
Hybrid molecules maintain their stronghold in the drug market, with over 60% of drug candidates in pharmaceutical industries. The substantial expenses for developing and producing biologically privileged drugs are expected to create opportunities for producing hybrid molecule-based drugs. Therefore, we have developed a simple and efficient copper-catalyzed approach for synthesizing a wide range of triazole-linked glycohybrids derived from pyrazolo[1,5-a]pyrimidines. Employing a microwave-assisted copper-catalyzed approach, we developed a concise route using various 7-O-propargylated pyrazolo[1,5-a]pyrimidines and 1-azidoglycosides. This strategy afforded a series of twenty-seven glycohybrids up to 98% yield with diverse stereochemistry. All were achieved within a remarkably shortened time frame. Our investigation extends to evaluating the anticancer potential of these synthesized triazole-linked pyrazolo[1,5-a] pyrimidine-based glycohybrids. In-vitro assays against MCF-7, MDA-MB231, and MDA-MB453 cell lines reveal intriguing findings. (2R,3S,4S,5R,6R)-2-(acetoxymethyl)-6-(4-(((5-(4-chlorophenyl)pyrazolo[1,5-a]pyrimidin-7-yl)oxy)methyl)-1H-1,2,3-triazol-1-yl)tetrahydro-2H-pyran-3,4,5-triyl triacetate emerges as a standout with better anticancer activity against MDA-MB231 cells (IC50 = 29.1��M), while (2R,3R,4S,5R,6R)-2-(acetoxymethyl)-6-(4-(((5-(4-chlorophenyl)pyrazolo[1,5-a]pyrimidin-7-yl)oxy)methyl)-1H-1,2,3-triazol-1-yl)tetrahydro-2H-pyran-3,4,5-triyl triacetate demonstrates the best inhibitory effects against MCF-7 cells (IC50 = 15.3��M) in all derived compounds. These results align with our docking analysis and structure�activity relationship (SAR) investigations, further validating the in-vitro outcomes. This work not only underscores the synthetic utility of our devised protocol but also highlights the promising potential of these glycohybrids as candidates for further anticancer therapeutic exploration. � 2024, The Author(s).
Impact of Bisphenol-A in the environment and its removal through biological agents: A review
(John Wiley and Sons Inc, 2024) Gupta A.; Singh A.; Mishra V.K.
Endocrine-disrupting chemicals (EDCs) are synthetic compounds that can cause abnormalities in the functioning of the endocrine system of the human being, resulting in various health problems. Bisphenol-A (BPA) is one of the important EDCs that is used in the manufacturing of plastics, food packaging canisters, and other day-to-day necessities. Since BPA is estrogenic in nature; its presence can affect the living component at a very low concentration. BPA and other EDCs are released from various sources like the plastic manufacturing industry, sewage treatment plants, and solid waste disposal points. Due to its global use, BPA has permeated different matrices, including our food sources. As a result, many adverse health effects have been reported, considering which, their treatment is necessary. Under the present investigation, we have reviewed various studies dealing with the sources, concentrations, and impact of BPA on the environment. In addition, we have also incorporated different treatment technologies including phytoremediation and bioremediation which can remove BPA effectively. Regardless of significant developments in our understanding of EDCs, uncertainties and knowledge gaps still occur and more studies are required to eradicate the problems of BPA contamination from our environment. � 2024 Wiley Periodicals LLC.
Investigation of treatment potential of horizontal subsurface flow constructed wetland for the treatment of secondary treated sewage
(Institute for Ionics, 2024) Shukla R.; Gupta D.; Mishra V.K.
Present study investigated the potential of different setups of horizontal subsurface flow constructed wetland (HSSFCW) for sewage treatment in the Indian context. Total four different setups, i.e., CW1 (unplanted CW); CW2 (planted with C. Benghalensis); CW3 (planted with T. Latifolia); and CW4 (planted with C. Benghalensis and T. Latifolia; 50% each of both the plants) were used to perform the treatment experiments. To perform the treatment experiment, a HSSFCW with the surface area of 35�m2 (3.5 � 10�m) and gravel as filter medium was built. Each setup of wetland remained operational for three months during which its performance was evaluated. The CW was fed at an average flow rate of 250�L�h?1 with secondary treated sewage, and the treated sewage was examined twice a week at five different hydraulic retention times (HRT), i.e., 0, 12, 24, 36 and 48�h for essential sewage quality parameters. The wetlands efficiency improved proportionally with the increase in the duration of the hydraulic retention time. However, the aquatic macrophytes enhanced the HSSFCW efficiency. The CW4, i.e., the wetland planted with combination of C. Benghalensis and T. Latifolia showed the best performance in treating the biological oxygen demand, chemical oxygen demand, nitrate, ammonia, and phosphate, i.e., 73%, 70%, 81%, 88%, and 67%, respectively. This setup also showed the best performance in treating the total coliforms, fecal coliforms, and E. coli with removal efficiency of 80%, 85%, and 83%, respectively, at 48�h HRT. � 2023, The Author(s) under exclusive licence to Iranian Society of Environmentalists (IRSEN) and Science and Research Branch, Islamic Azad University.
Isolation and characterization of hexavalent chromium-tolerant endophytic bacteria inhabiting Solanum virginicum L. roots: A study on potential for chromium bioremediation and plant growth promotion
(Elsevier B.V., 2024) Gupta A.; Gond S.K.; Mishra V.K.
Present study was performed with the aim to isolate Heavy metal Tolerant- PGPB (HMT-PGPB) from metal-contaminated site and use them for Cr bioremediation. Six different bacterial strains were obtained from the endosphere of Solanum virginicum L. roots and cultured using nutrient agar media amended with 20 mg/L of Cr(VI). The ability of these Cr(VI) tolerant bacterial isolates were assessed for PGP traits like producing siderophores, indole-3-acetic acid (IAA), and phosphate solubilization. The findings indicated that all of the isolates could produce exopolymeric substances and IAA, five of them could produce siderophores, and three could solubilize phosphate. Furthermore, the minimum inhibitory concentrations of these strains werealso determined. These strains were identified as Bacillus licheniformis SxR1, B. tequilensis SxR2, B. subtilis SxR3, B. velezensis SxR4, B. amyloliquefaciens SxR6, and B. stercoris SxR8. To validate the findings, it is crucial to comprehend how Cr(VI) affects Bacillus sp. SxR1 cells to determine the course of uptake and bacterial cell alteration, which was assessed via Fourier Transform-Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). � 2024 The Authors
Macrophytes for Utilization in Constructed Wetland as Efficient Species for Phytoremediation of Emerging Contaminants from Wastewater
(Springer Science and Business Media B.V., 2024) Singh P.; Singh G.; Singh A.; Mishra V.K.; Shukla R.
Emerging contaminants�(EC) are the modern age chemicals that are new to the environment. It includes pharmaceuticals & personal care products (PPCPs), pesticides, hormones, artificial sweeteners, industrial chemicals, microplastics, newly discovered microbes and many other manmade chemicals. These chemicals are harmful and having negative impacts on human being and other life forms. Existing treatment systems are ineffective in treating the EC and the treated effluent act as source of pollution to the water bodies. Considering the requirement of new technologies that can remove EC, the Constructed wetlands (CWs) are getting popular and can be a valid option for the treatment of EC. In this context application of macrophytes in CW have increased the removal performance of constructed wetland system. Growing macrophytes in CW have augmented the removal of EC from these systems. In different studies macrophytes supported the removal process of EC in CW and a removal efficiency up to 97% was achieved. This review summarizes the direct and indirect roles of macrophytes in CW in the treatment of EC. Also, it evaluates the success of CW technology, in treating EC, its limitation, and future perspective. The direct role of macrophytes include precipitation on root surface, absorption, and degradation of EC by these plants. Growth of macrophytes in CWs facilitates the uptake EC by the absorption and detoxify them in their cell with the help of enzymatic and hormonal activity which supports the removal of EC in wetland system. Indirect impacts, which appear to be more significant than direct effects, include increased removal of EC through better rhizospheric microbial activity and exudate secretions, which enhances the removal by four times. Thus, this review emphasizes combined application of CW and aquatic macrophytes which augmented the performance of CW for the treatment of EC. Graphical Abstract: [Figure not available: see fulltext.]. � 2024, The Author(s), under exclusive licence to Society of Wetland Scientists.
Microwave-Assisted Copper-Catalyzed Synthesis of Triazole-linked 2-Amino-Pyrimidinone based Glycohybrids
(John Wiley and Sons Inc, 2024) Mishra V.K.; Khanna A.; Tiwari G.; Yadav Y.; Sagar R.
2-Amino pyrimidines are vital molecules that exhibit diverse biological activities and serve as structural motifs in various pharmaceutical drugs. Four new series of triazole-linked glycohybrids of 2-amino-pyrimidinones were designed and efficiently synthesized, using a microwave-assisted method under click chemistry reaction conditions. The N-propargyl-2-aminopyrimidinone and O-propargyl-2-aminopyrimidine derivatives successfully underwent the click reactions with 1-azido-tetra-O-acetyl-?-D-glucose, D-galactose and D-mannose. In this work, we reported the series of novel thirty-three new triazole-linked glycohybrid molecules with stereochemical diversity and diverse substitution patterns. � 2024 Wiley-VCH GmbH.
Potential of different biobed�biopurification systems in the treatment of domestic sewage
(IWA Publishing, 2024) Singh A.; Singh G.; Singh P.; Mishra V.K.
In total, four biobed-biopurification systems (BBPS) were made for treating the wastewater: BBPS1, BBPS2, BBPS3 and BBPS4, and the substrates used in biobeds were rice husk, soil, vermicompost, gravel and sand as an adsorbent in different proportion according to the design and treatment needs. Five litres of primary treated effluent were provided to every setup and the effluents were analysed after different retention intervals, i.e., 0 h, 24 h, 48 h and 72 h for different physio-chemical properties. The findings of the experiment showed that the BBPS1, BBPS2, BBPS3 and BBPS4 was highly efficient to remove organic impurities but much less efficient to eliminate physical impurity. Much faster removal of the pollutants was achieved in the BBPS1 and BBPS2 in comparison to BBPS3 and BBPS4. Both the beds BBPS1 and BBPS2 created favourable circumstances for organic contaminants to biodegrade as for biological oxygen demands (BOD) removal efficiency was 55.35% and 56.44% and chemical oxygen demands (COD) removal efficiency was 85.15% and 70.90%, respectively. Both the setups, i.e., BBPS1 and BBPS 2 are also much more efficient for the removal of biogenic contaminants, i.e., 85.71% and 73.20% for nitrate and 65.12% and 76.99% for phosphate, respectively. Overall, the performance of BBPS2 proved excellent in comparison to other setups by calculation of its removal efficiency percent for different parameters. � 2024 The Authors.
Raman signature of the interaction between functionalized MWCNT and the liquid crystalline system (4DBA)
(American Institute of Physics, 2024) Khoja U.B.; Patel C.B.; Srivastava R.; Mishra V.K.; Tripathi P.; Singh A.R.; Singh R.K.; Vikram K.
Liquid crystalline systems provide the best molecular matrix as a host for carbon nanotubes (CNTs) due to their fluidity character that controls the molecular ordering. The anisotropic shape of the CNTs makes them highly suitable nanoparticles that can be embedded in liquid crystalline systems to achieve uniform tunable dispersion. The uniform and stable dispersion of CNTs in the liquid crystalline host is challenging due to strong Vander Waal forces between CNTs. The functionalization of CNTs is one of the most popular techniques to achieve the dispersion system in an LC host due to the covalent bonding between CNT and LC systems. The COOH functionalized multi-wall carbon nanotubes (F-MWCNT) provide effective sites to interact with LC systems with the carboxylic group at the terminal. Raman spectroscopy is used as a probe to investigate the interaction between 4DBA and F-MWCNT through COOH - -COOH dimer formation. Comparative Raman analysis of 4DBA, 4DBA + bare MWCNT, and 4DBA + F-MWCNT spectra show bands associated with the COOH group giving strong evidence of interaction between 4DBA and F-MWCNT. Raman study reveals the mechanism of dimer formation between F-MWCNT and 4DBA. � 2024 Author(s).
Recent developments on the synthesis of biologically active glycohybrids
(Academic Press Inc., 2024) Mishra V.K.; Khanna A.; Tiwari G.; Tyagi R.; Sagar R.
The exploration of hybridization emerges as a potent tool in advancing drug discovery research, with a significant emphasis on carbohydrate-containing hybrid scaffolds. Evidence indicates that linking carbohydrate molecules to privileged bioactive scaffolds enhances the bioactivity of drug molecules. This synergy results in a diverse range of activities, making carbohydrate scaffolds pivotal for synthesizing compound libraries with significant functional and structural diversity. Beyond their synthesis utility, these scaffolds offer applications in screening bioactive molecules, presenting alternative avenues for drug development. This comprehensive review spanning 2015 to 2023 focuses on synthesized glycohybrid molecules, revealing their bioactivity in areas such as anti-microbial, anti-cancer, anti-diabetic, anti-inflammatory activities, enzyme inhibition and pesticides. Numerous novel glycohybrids surpass positive control drugs in biological activity. This focused study not only highlights the diverse bioactivities of glycohybrids but also underscores their promising role in innovative drug development strategies. � 2024 Elsevier Inc.
Revolutionizing remediation: Unveiling the power of Lysinibacillus sp. in tackling heavy metal stress
(Elsevier B.V., 2024) Gupta A.; Siwach C.; Mishra V.K.
Increased industrialization has elevated heavy metal pollution in soil. Considering their hazardous impact on the ecosystem, it is important to lower heavy metals concentration from the polluted soil. Standard physicochemical methods for metal remediation are quick, effective, and operational; nevertheless, they come with a high cost and are not appropriate for large contaminated areas. They require a lot of energy, produce a large amount of toxic sludge, are not practical for low metal concentrations, and are not eco-friendly. An effective technique for eradicating hazardous heavy metals from contaminated soil is desperately needed given the state of environmental degradation that exists today. Using microorganisms, particularly bacteria resistant to hazardous metals, is the most economical method known as bioremediation. Owing to the enduring characteristics of heavy metals in polluted soil, inhabitant microorganisms require bioengineering in order to assess an appropriate biotechnical method for the removal and/or detoxification of heavy metals in contaminated soil. Many microorganisms have been reported with the ability to alleviate heavy metals from a contaminated environment; Lysinibacillus being one of them. This review highlights the efficiency of Lysinibacillus in heavy metal remediation and how exactly this genus dealt with metal stress at the cellular level. We have evaluated various studies of bioremediation by this particular genus and also illuminated its plant growth-promoting properties. � 2024 The Authors
Simulating and predicting surface water quality for drinking and bathing purposes through combined approach of PCA, entropy-based WQI, and stochastic models
(Springer Science and Business Media Deutschland GmbH, 2024) Chaudhary S.; Duraiyarasan A.; Gupta D.; Mishra V.K.
Freshwater resources, specially surface water are under threat due to over extraction, discharge of pollutants and improper waste disposal. This study investigates the present status of water quality of the River Ganga at Varanasi, India and further predict its future status using Principal Component Analysis (PCA), Entropy water quality index (EWQI) and Stochastic models. To begin with, water quality data of 37 variables for eleven years were acquired followed by which PCA was applied which reduced the number of water quality variables from 37 to 13. EWQI of River Ganga was calculated for drinking and bathing purposes by using these 13 variables. Most of the physico-chemical variables were within the permissible limit. The EWQI values were calculated for all the samples indicated that none of the water sample was suitable for drinking without treatment. However, 74.24% of the samples were classified as fair, 10.60% as poor, and 15.15% as unfit for bathing. Five different time-series models with 10 different structures were accessed then compared the effectiveness of various time series models in predicting water quality with the help of data from the past eleven years. Finally, the chosen models were used to predict future water quality variables and EWQI for both drinking and bathing purposes. The optimized models were selected based on auto-correlation function and partial auto-correlation function as well as the use of Akaike information criteria and Bayesian Information Criteria. This research suggests that time series modelling can be a cost-effective and time-saving approach for long-term water quality monitoring. Graphical abstract: (Figure presented.) � The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
Spatio-temporal evaluation of drought adaptation in wheat revealed NDVI and MTSI as powerful tools for selecting tolerant genotypes
(Elsevier B.V., 2024) Reddy S.S.; Singh G.M.; Kumar U.; Bhati P.; Vishwakarma M.; Navathe S.; Yashavanthakumar K.J.; Mishra V.K.; Sharma S.; Joshi A.K.
Context: Water is one of the major limiting factors for wheat production. Mult-environmental evaluation is necessary to identify stable drought tolerant wheat genotypes. Objectives: To identify stable drought tolerant wheat genotypes and reliable phenotypic and/or spectral markers for drought tolerance. Methods: One hundred ninety-six diverse wheat genotypes were evaluated at three different locations in India for two years (E1 to E12). Drought was imposed at the heading stage (Z59) by withholding irrigation until the moisture content reached <45% as compared to the control (100%). Various Morpho-physiological and phenological traits: Days to flowering (DTF) and maturity (DTM), plant height (PH), grain yield (GY), NDVI, canopy temperature depression (CTD), and chlorophyll readings were recorded. Different stress indices and stability models (AMMI - Additive Main Effects and Multiplicative Interaction; WAASB - Weighted Average of Absolute Scores from the singular value decomposition of the matrix of BLUPs; and MTSI - Multi-Trait Stability Index) were used to identify the stable and tolerant genotypes. In addition, discriminate function analysis (DFA) was performed to identify drought tolerant genotypes. Results: Genotype performance reduced significantly under drought for all traits in all environments. Overall, GY was reduced by 35% under drought compared to control. Pooled ANOVA showed that 81% of the variation in grain yield was due to the environment and 10.6% due to its interaction with genotypes. MTSI and WAASBY identified 11 common genotypes with stable performance across all environments. Further, 29 stable genotypes selected by MTSI (with 15% selection intensity) had higher selection differential than other stability models. Further, NDVI at maturity showed a positive and significant correlation [r = 0.41** in E2 and 0.36** in E4) with the GY specifically under drought for two years. Conclusion: MTSI is an effective method for selecting stable wheat genotypes under drought conditions. NDVI may be a high throughput screening tool for drought tolerance. Implications: MTSI may be used to identify stable genotypes, while DFA is useful in selecting drought tolerant genotypes. Further, NDVI can be used in addition to yield traits to screen wheat genotypes for drought tolerance. � 2024 Elsevier B.V.
Synthesis of Chirally Enriched Pyrazolylpyrimidinone-Based Glycohybrids via Annulation of Glycals with 2-Hydrazineylpyrimidin-4(3H)-ones
(American Chemical Society, 2024) Tiwari G.; Mishra V.K.; Khanna A.; Tyagi R.; Sagar R.
A new strategy for synthesizing chirally enriched pyrazolylpyrimidinone-based glycohybrids has been achieved, employing an annulation approach in ethanol without any additives or catalysts under microwave conditions. The designed compounds were obtained within a short reaction time (5 min). This method offers several advantages, including mild reaction conditions, a green solvent, and a metal-free approach. Furthermore, the protocol demonstrated a broad substrate scope, successfully incorporating various functional groups with stereochemical diversity and furnishing chirally enriched molecules. � 2024 American Chemical Society.
Synthesis of triazole bridged N-glycosides of pyrazolo[1,5-a]pyrimidinones as anticancer agents and their in silico docking studies
(Royal Society of Chemistry, 2024) Tiwari G.; Mishra V.K.; Kumari P.; Khanna A.; Sharma S.; Sagar R.
In the pursuit of novel therapeutic agents, we present a comprehensive study on the design, synthesis, and evaluation of a diverse library of triazole bridged N-glycosides of pyrazolo[1,5-a]pyrimidinones, employing a microwave-assisted synthetic approach via �click chemistry�. This methodology offers efficient and accelerated access to the glycohybrids, showcasing improved reaction conditions that yield high-quality products. In this research endeavor, we have successfully synthesized a series of twenty-seven triazole bridged N-glycosides of pyrazolo[1,5-a]pyrimidinones. Our investigation extends beyond synthetic endeavors to explore the potential therapeutic relevance of these compounds. We subjected them to rigorous in vitro screening against prominent breast cancer cell lines MCF-7, MDA-MB231, and MDA-MB453. Among the library of compounds synthesized, (2S,3S,4R,5S,6S)-2-(acetoxymethyl)-6-(4-((5-(4-methoxyphenyl)-7-oxopyrazolo[1,5-a]pyrimidin-1(7H)-yl)methyl)-1H-1,2,3-triazol-1-yl)tetrahydro-2H-pyran-3,4,5-triyl triacetate emerged as a potent compound, exhibiting remarkable anti-cancer activity with an IC50 value of 27.66 ?M against the MDA-MB231 cell line. Additionally, (2S,3R,4R,5S,6S)-2-(acetoxymethyl)-6-(4-((7-oxo-5-(4-(trifluoromethyl)phenyl)pyrazolo[1,5-a]pyrimidin-1(7H)-yl)methyl)-1H-1,2,3-triazol-1-yl)tetrahydro-2H-pyran-3,4,5-triyl triacetate displayed notable inhibitory potential against the MCF-7 cell line, with an IC50 value of 4.93 ?M. Furthermore, in silico docking analysis was performed to validate our experimental findings. These findings underscore the promise of our triazole bridged N-glycosides of pyrazolo[1,5-a]pyrimidinones as potential anti-cancer agents. This research not only enriches the field of glycohybrid synthesis but also contributes valuable insights into the development of novel anti-cancer therapeutics. � 2024 The Royal Society of Chemistry.

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