Browsing by Author "Tiwary S.K."

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    Deep vein thrombosis: does platelet hyperaggregation have any role?
    (Edizioni Minerva Medica, 2024) Tiwary S.K.; Das A.K.; Kumar P.; Dash D.; Khanna A.K.
    [No abstract available]
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    Gene expression study in venous ulcer
    (Edizioni Minerva Medica, 2024) Tiwary S.K.; Sharma A.; Priya K.; Rai G.; Katiyar V.K.; Kumar P.; Khanna A.K.
    BACKGROUND: Venous leg ulcers are responsible for more than half of all lower extremity ulcerations. Therefore, in this study we have proposed to understand the genetic changes associated with underlying disease mechanisms in venous ulcer by evaluating the gene expression of inflammatory gene/marker WAKMAR1, E2F1, RAD21 and NIPBL. METHODS: WAKMAR1, E2F1, RAD21 and NIPBL genes sequence was pulled out from NCBI database. The gene-specific primers were designed using Primer 3 software and were synthesized. Tissue sample collected before starting treatment and after 50% healing or after 2-6 months in non- healing ulcers. Samples stored at -80 �C, were thawed and dissected into small pieces. In all samples RNA was isolated and quantified using a Nano Drop. Standard 1% agarose gel under denaturing condition with ethidium bromide was used to assess the integrity of RNA. WAKMAR1, E2F1, RAD21 and NIBPL gene expression was directly analyzed by one step RT-PCR. Gene expression was made relative to expression of house-keeping beta actin gene. Statistical comparisons performed using Student�s t-test with the help of GraphPad Prism version 8.0.2, NIH. RESULTS: RAD21 gene expression increased significantly in tissue samples from venous ulcer patients post treatment. NIPBL gene expression increased but not significantly in tissue samples from venous ulcer patients post treatment. Relative expression of RAD21 gene significantly increased in healing venous ulcer samples as compared to non-healing samples (P<0.05 in healing v/s non-healing group). Relative expression of NIPBL gene significantly increased in healing venous ulcer samples as compared to non-healing samples (P<0.0001 in healing v/s non-healing group). No expression for WAKMAR1 and E2F1 were noticed in venous ulcer tissue samples. CONCLUSIONS: NIPBL & RAD21 gene expression increased significantly in healed venous ulcers. This finding could be harnessed for the development of more effective wound therapy. � 2024 EDIZIONI MINERVA MEDICA.
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    Portuguese patrimony in India: Continuity of the past into the present
    (Archaeopress, 2024) Tiwary S.K.; Prakash O.
    When the land route from Europe to India was lost after the fall of Constantinople in 1453, the need arose to discover the sea route to India. The Portuguese succeeded in finally discovering the much- coveted sea route to India when Vasco Da Gama reached at the Indian shores in Calicut on May 20th of 1498. However, soon other European countries started sending their merchants to India which led to competition and conflict among the European powers over the issue of control of trade in India for cotton, sugar, spices, and labourers. Subsequently, the mainland of India fell in control of the British East India Company and the Portuguese were restrained on the western coast of India, where they finally settled in Goa which became the headquarter of the Portuguese from where they controlled and exercised a full monopoly over the spice trade from the South-East Asian countries. With this arose the 'Indo-Portuguese Cultural Exchanges' between India and Portugal which lasted more than four centuries starting in 1505 and lasting to 1961. Thus, surpassing the 200 long British Rule in India. The purpose of the proposed paper is to highlight some of the important influences and exchanges between the Indian and Portuguese cultures that occurred during the centuries-long cohabitation of the Indian and Portuguese people on Indian soil. However, the relationship was not always cordial as it also had some occasional doses of conflict. Nevertheless, the smallest state of Indian republic, Goa, became the controlling centre of the Portuguese for the rest of the eastern world like Korea, Japan, Indonesia, Bangladesh, etc for more than 400 years. And it is quite interesting to note that the language, trade, architectural designs were some of the influences between the Portuguese and Indians. India is the only place where the Portuguese building heritage is nominated in the UNESCO World Heritage Sites. � the individual authors and Archaeopress 2024. All rights reserved.
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    Reconstructing the population history of the�Nicobarese
    (Springer Nature, 2024) Mishra R.K.; Singh P.P.; Rai N.; Desai S.; Pandey P.; Tiwary S.K.; Tamang R.; Suravajhala P.; Shrivastava P.; Thangaraj K.; van Driem G.; Chaubey G.
    The Nicobarese are the major tribal groups in the Nicobar district, situated south of the Andaman group of Islands. Linguistic phylogeny suggests that the linguistic ancestors of the Nicobarese settled the Nicobar archipelago in the early Holocene. So far, genetic research on them is low-resolution and restricted to the haploid DNA markers. Therefore, in the present analysis, we have used the high-resolution biparental (1554 published and 5 newly genotyped Nicobarese individuals) and uniparental genetic markers and looked at the genetic association of Nicobarese with the South and Southeast Asian populations. We report a common ancestral component shared among the Austroasiatic of South and Southeast Asia. Our analyses have suggested that the Nicobarese peoples retain this ancestral Austroasiatic predominant component in their genomes in the highest proportion. On the Southeast Asian mainland, the Htin Mal, who speak an Austroasiatic language of the Khmuic branch, represent a population that has preserved their ethnic distinctness from other groups over time and consequently shown the highest drift with the Nicobarese. The analysis based on haplotypes indicated a significant level of genomic segment sharing across linguistic groups, indicating an ancient broader distribution of Austroasiatic populations in Southeast Asia. Based on the temporal analyses of haploid DNA, it is suggested that the forebears of the Nicobarese people may have arrived on the Nicobar Islands in the last 5000 YBP. Therefore, among the modern populations, the Nicobarese peoples and the Htin Mal language community represent good genetic proxies for ancient Austroasiatics. � The Author(s), under exclusive licence to European Society of Human Genetics 2024.
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    The maternal genetic history of tribal populations of Chhattisgarh, India
    (Elsevier B.V., 2024) Dixit S.; Shrivastava P.; Jeevan Sequeira J.; Mustak M.S.; Rana M.; Kushwaha P.; Shrivastava D.; Kumawat R.K.; Pratap Singh P.; Tiwary S.K.; Chauhan N.K.; Chaubey G.
    The central region of India boasts a rich tribal heritage and the highest number of tribal populations in the country. Analysing the genetic history of this population can offer valuable insights into various demographic processes that shaped the gene pool of present-day settlers of this region. In this study, we utilize a recently validated Next-generation sequencing (NGS) technique to sequence 24 tribal mitogenomes from the Chhattisgarh population for genetic ancestry and forensic analysis. The identified ancient haplogroups in this population can be traced back to the pre-Last Glacial Maximum (LGM) period. Our Bayesian analysis provides evidence for maternal ancestral expansion following the earliest Out-of-Africa migration, followed by a prolonged steady phase. We identified three basal founding haplogroups, M2, R5, and U2 in the Chhattisgarh region that diversified during the Neolithic period. Indistinct distribution pattern of these haplogroups among tribes and castes suggests that the maternal ancestry of Chhattisgarh population predates any kind of social stratification that exists today in the Indian subcontinent. Furthermore, our analysis suggests that this region remained unaffected by the Last Glacial Maximum. The forensic analysis of the mitogenomes demonstrates a high power of discrimination (0.9256) within the Chhattisgarh population, thus supporting the applicability of mitogenome NGS technology in forensic contexts. � 2024 Elsevier B.V. and Mitochondria Research Society