Gene expression study in venous ulcer
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Date
2024
Journal Title
Acta Phlebologica
Journal ISSN
Volume Title
Publisher
Edizioni Minerva Medica
Abstract
BACKGROUND: Venous leg ulcers are responsible for more than half of all lower extremity ulcerations. Therefore, in this study we have proposed to understand the genetic changes associated with underlying disease mechanisms in venous ulcer by evaluating the gene expression of inflammatory gene/marker WAKMAR1, E2F1, RAD21 and NIPBL. METHODS: WAKMAR1, E2F1, RAD21 and NIPBL genes sequence was pulled out from NCBI database. The gene-specific primers were designed using Primer 3 software and were synthesized. Tissue sample collected before starting treatment and after 50% healing or after 2-6 months in non- healing ulcers. Samples stored at -80 �C, were thawed and dissected into small pieces. In all samples RNA was isolated and quantified using a Nano Drop. Standard 1% agarose gel under denaturing condition with ethidium bromide was used to assess the integrity of RNA. WAKMAR1, E2F1, RAD21 and NIBPL gene expression was directly analyzed by one step RT-PCR. Gene expression was made relative to expression of house-keeping beta actin gene. Statistical comparisons performed using Student�s t-test with the help of GraphPad Prism version 8.0.2, NIH. RESULTS: RAD21 gene expression increased significantly in tissue samples from venous ulcer patients post treatment. NIPBL gene expression increased but not significantly in tissue samples from venous ulcer patients post treatment. Relative expression of RAD21 gene significantly increased in healing venous ulcer samples as compared to non-healing samples (P<0.05 in healing v/s non-healing group). Relative expression of NIPBL gene significantly increased in healing venous ulcer samples as compared to non-healing samples (P<0.0001 in healing v/s non-healing group). No expression for WAKMAR1 and E2F1 were noticed in venous ulcer tissue samples. CONCLUSIONS: NIPBL & RAD21 gene expression increased significantly in healed venous ulcers. This finding could be harnessed for the development of more effective wound therapy. � 2024 EDIZIONI MINERVA MEDICA.
Description
Keywords
Gene expression, Varicose ulcer, Wound healing, Wounds and injuries